Phosphate solubilizing bacterium and extraction method thereof

Abstract

The invention discloses a phosphate solubilizing bacterium and an extraction method thereof. The phosphate solubilizing bacterium is named a strain B5, the strain B5 is a pseudomonas bacterium, determination results of biochemical criterion are gram staining is negative, a methyl red test is negative, a V-P reaction is negative, an indole test is negative, an oxidase test is negative, the strain B5 can use citrate as a carbon source to produce extracellular enzyme-gelatinase liquidation gelatin, catalase and indole are produced, and H2S is generated; the order of the phosphate solubilizing ability of the strain B5 from high to low under different carbon sources is that glucose is greater than galactose, the galactose is greater than lactose, the lactose is greater than sucrose, and the sucrose is greater than soluble phosphate starch, and the order of the phosphate solubilizing ability under different nitrogen sources is that ammonium sulfate is greater than ammonium chloride, the ammonium chloride is greater than urea, and the urea is greater than potassium nitrate; and the optimum growth temperature of the strain B5 is 28-32 DEG C, and the optimum growth pH of the strain B5 is 7-9.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a phosphate solubilizing bacterium and an extraction method thereof. Background technique [0002] One of the main ways for plants to obtain phosphorus from the soil is to provide the phosphorus needed for their own growth. However, although the phosphorus content in the soil is 0.05% (w / w), only 0.1% of the soluble phosphorus can be absorbed by plants, and the phosphorus content absorbed by phosphorus in the soil is far less than the phosphorus content required for plant growth. In the process of agricultural production, phosphorus in the soil is very easy to form insoluble phosphate and complexes with soil components, resulting in less soil phosphorus available to plants and a low utilization rate of phosphorus fertilizer, so the growth of crops severely suppressed. Not only that, a large amount of phosphorus fertilizer application will degrade soil ferti...

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Problems solved by technology

[0005] Phosphorus solubilizing bacteria in different soils have different phosphorus solubilizing abilities. There are many inland areas in Xinjiang and imported strains from abroad, but due to inadaptability to the local environment, most of the bacterial agents cannot play a role

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