Method for preparing hyperoxide dismutase by fermenting shiraia bambusicola

A technology of superoxide and bamboo yellow fungus, applied in the field of bioengineering, can solve the problems of unfavorable large-scale production and preparation, high operating costs, and achieve the effects of high medicinal value and health care efficacy, short fermentation period and broad development prospects.

Inactive Publication Date: 2010-02-03
江苏竹红生物科技有限公司
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  • Abstract
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Problems solved by technology

[0004] At present, there are relatively few strains that can produce SOD, mainly some yeasts, and they are intracellular enzymes, which need to be broken down to extract and separate, and the operation cost is high, which is not conducive to large-scale production and preparation

Method used

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  • Method for preparing hyperoxide dismutase by fermenting shiraia bambusicola

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Effect test

Embodiment 1

[0030] The composition of the medium is: potato juice 200g / L, glucose 20g / L, beef extract 10g / L, peptone 10g / L, NH 4 NO 3 10g / L, corn steep liquor powder 12g / L, natural pH, 10% inoculum size, 30°C fermentation temperature, 300L air-lift fermenter, 70% liquid volume, 1:1.5v / v / m ventilation , the fermentation time is 3 days. After 24 hours of fermentation, add H to the fermentation broth 2 o 2 1 μmol / L, Tween 60 0.8% and tetradecane 2.5%, and continuously give 10000Lux light until the end of fermentation. Under this condition, the activity of SOD in the fermentation broth was 800U / mL, and that in the cell was 5U / mL. The yield of perylenequinone pigments was 1.14g / L, of which hypocrellin A accounted for 90%, and hypocrellin B accounted for 5%.

Embodiment 2

[0032] The composition of the medium is: fructose 20g / L, beef extract 20g / L, corn syrup powder 30g / L, pH natural. The inoculum size is 5%, the fermentation temperature is 24°C, the 300L air-lift fermenter, the liquid filling capacity is 70%, the ventilation rate is 1:0.3v / v / m, and the fermentation time is 5 days. No surfactants, alkanes, H 2 o 2 . No light is applied. Under this condition, the activity of SOD in the fermentation broth was 10 U / mL, and that in the cell was 1 U / mL. The yield of perylenequinone pigments was 0.31g / L, of which hypocrellin A accounted for 91%, and hypocrellin B accounted for 4%.

Embodiment 3

[0034] The medium composition is: potato juice 200g / L, lactose 20g / L, NH 4 NO 3 10g / L, peptone 10g / L, pH natural. The inoculum size is 10%, the culture temperature is 30°C, the 500L mechanically stirred fermenter, the liquid filling capacity is 60%, the ventilation rate is 1:0.5v / v / m, the stirring speed is 150r / min, and the fermentation time is 4 days. When the fermentation started, immediately add Triton X-100 1.5% to the fermentation broth. Under this condition, the SOD activity in the fermentation broth was 321U / mL, and the activity in the cell was 3U / mL. The yield of perylenequinone pigments was 0.53g / L, of which hypocrellin A accounted for 93%, and hypocrellin B accounted for 3%.

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Abstract

A method for preparing hyperoxide dismutase (SOD) by fermenting shiraia bambusicola belongs to the technical field of bioengineering. The method adopts shiraia bambusicola strain for preparing the SODwhich is mainly exoenzyme under the condition of liquid state fermentation. During the fermentation, the SOD yield can be improved by adding H2O2, surfactant or alkane or by illumination. The maximumSOD yield can be 800 U/mL. During the fermentation, the strain can simultaneously generate quinochromes which can have a yield as high as 1.1 g/L, wherein hypocrellin A accounts for 90 percent and hypocrellin B accounts for 5 percent. Tabasheer is an officinal fungus and mainly generates hypocrellins and other quinochromes by means of fermentation, thereby having high officinal value and healthcare function. The fermentation of the tabasheer to prepare the SOD has the advantage of short fermentation period, the activity of the SOD in fermentation liquid is relatively high, and the quinochromes can be simultaneously generated. The method has a good prospect for industrial application.

Description

technical field [0001] The invention discloses a method for fermenting and producing superoxide dismutase (SOD) by bamboo yellow fungus, which belongs to the technical field of bioengineering. The invention relates to obtaining SOD through liquid state fermentation by using bamboo yellow strain Shiraia sp. SUPER-H168 with preservation number CCTCCM 207104. Background technique [0002] Superoxide dismutase (SOD) is an enzyme that can scavenge superoxide anion free radicals. It plays an important role in the antioxidant system of organisms and widely exists in animals, plants, all aerobic microorganisms and a few an Oxygen in microorganisms. SOD can improve the body's resistance to free radical damage and resistance to free radical-inducing factors, has obvious anti-aging, enhances the adaptability to overload, and has anti-inflammatory and anti-tumor effects, especially for rheumatism Arthritis and senile dark spots have significant preventive effects, and have been widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12P7/66C12R1/645
Inventor 蔡宇杰廖祥儒梁晓辉魏兆媛丁彦蕊孟强王亚辉张大兵
Owner 江苏竹红生物科技有限公司
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