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Method for increasing content of propionic acid in batch fermentation acid-producing liquid

A technology for fermentation supernatant and propionic acid, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of insufficient content, difficult to meet the needs of high-efficiency biological phosphorus removal and nitrogen removal, etc. high content

Active Publication Date: 2012-11-14
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the content of soluble COD (including SCFAs, especially acetic acid and propionic acid) in the influent water of many southern urban sewage plants in my country is insufficient, and it is difficult to meet the needs of efficient biological phosphorus and nitrogen removal.

Method used

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  • Method for increasing content of propionic acid in batch fermentation acid-producing liquid
  • Method for increasing content of propionic acid in batch fermentation acid-producing liquid
  • Method for increasing content of propionic acid in batch fermentation acid-producing liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Such as figure 1 Shown:

[0032] (1) Mixture of pre-fermented sludge and kitchen waste (first-stage fermentation);

[0033] Take the crushed and sieved kitchen waste (removing chopsticks, broken bones, plastics, paper pieces, debris, etc.) 10-mesh sieve to obtain concentrated kitchen waste with a C / N of 16-20; add tap water to make the water content 80%; the main components of kitchen waste are rice, supplemented by meat and oil, rice, meat and oil The optimal dry weight ratio is 7:2:1.) 0.41L, mixed with 0.46L concentrated excess sludge (TSS 20g / L, VSS 14g / L, moisture content 98%, pH=6.84) (sludge and The dry weight ratio of kitchen waste is 0.18:1), add 1.63L tap water, and inject it into a 3L anaerobic fermentation tank (TCOD is 25g COD / L);

[0034] Fermentation at room temperature (25°C) for 2.5 days with a stirring speed of 120rpm and using Ca(OH) 2 The pH was controlled to be 8.5; the fermentation mixture was taken out and centrifuged at 3000 rpm for 5 min to ...

Embodiment 2

[0041] (1) Mixture of pre-fermented sludge and kitchen waste (first-stage fermentation);

[0042]Take 0.37L of crushed and screened kitchen waste (removing chopsticks, broken bones, plastic, paper pieces, debris, etc.), and 0.7L of concentrated residual sludge (TSS 20g / L, VSS 14g / L, moisture content 98%, pH=6.84) mixed (dry weight ratio of sludge to kitchen waste is 0.3:1), add 1.43L tap water, inject into 3L anaerobic fermentation tank (TCOD is 25g COD / L);

[0043] Fermentation at room temperature (25°C) for 2.5 days with a stirring speed of 250rpm and using Ca(OH) 2 The pH was controlled to be 8; the fermentation mixture was taken out and centrifuged at 3000 rpm for 5 min to obtain 2 L of centrifuged supernatant.

[0044] (2) Activate and enrich Propionibacterium;

[0045] Propionibacterium (SICC1.256) was activated from the slant and then enriched and stored in 10% glycerol tube storage medium (see Table 1 for the medium formula), and the storage environment was -80°C; be...

Embodiment 3

[0050] (1) Mixture of pre-fermented sludge and kitchen waste (first-stage fermentation);

[0051] Take 0.45L of crushed and screened kitchen waste (removing chopsticks, broken bones, plastic, paper pieces, debris, etc.), and 0.22L of concentrated residual sludge (TSS 20g / L, VSS 14g / L, moisture content 98%, pH=6.84) mixed (dry weight ratio of sludge to kitchen waste is 0.08:1), add 1.83L tap water, inject into 3L anaerobic fermentation tank (TCOD is 25g COD / L);

[0052] Fermentation at room temperature (25°C) for 2.5 days with a stirring speed of 60rpm and using Ca(OH) 2 The pH was controlled to be 8; the fermentation mixture was taken out and centrifuged at 3000 rpm for 5 min to obtain 2 L of centrifuged supernatant.

[0053] (2) Activate and enrich Propionibacterium;

[0054] Propionibacterium (SICC1.256) was activated from the slant and then enriched and stored in a 15% glycerol tube storage medium (see Table 1 for the medium formula), and the storage environment was -80°C...

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Abstract

The invention belongs to the technical field of environmental protection and relates to a method for increasing content of propionic acid in batch fermentation acid-producing liquid. The method includes the steps of firstly, mixing kitchen waste and thickened sludge, adding mixture into an anaerobic hydrolysis fermenter for anaerobic fermentation, centrifugally separating mixture, and taking supernate for standby; secondly, activating and enriching propionibacterium seed broth; thirdly, sterilizing the supernate obtained in the step 1, inoculating the propionibacterium seed broth obtained in the step 2 to the supernate, and fermenting in an anaerobic fermenter to generate propionic acid; and fourthly, centrifuging the fermented mixture to obtain the fermented supernate rich in propionic acid after fermentation. The sludge and the kitchen waste are subjected to primary fermentation under the weak alkaline condition, and microorganism extracellular enzyme hydrolysis of macromolecular organic matters such as microorganism exoenzyme hydrolysate, protein and fat is facilitated. The content of the propionic acid in the fermented supernate is 3.56-9.95g COD per L of total acid in the supernate.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and relates to a method for increasing the content of propionic acid in batch fermentation acid production liquid. Background technique [0002] At present, the eutrophication of water body is becoming more and more serious. Controlling the amount of nitrogen and phosphorus in sewage is an important strategy to prevent water eutrophication in the world. The application of biological phosphorus and nitrogen removal technology can effectively reduce the content of phosphorus and nitrogen in sewage, and the volatile fatty acids in wastewater can provide the required carbon source for biological phosphorus and nitrogen removal. It has been reported (see Water Science and Technology, 1992, 25, 185-194) claimed that 6-9 mg of short-chain fatty acids (SCFAs) were required to treat 1 mg of phosphorus by biological methods. [0003] However, the content of soluble COD (including SCFAs, es...

Claims

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Application Information

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IPC IPC(8): C12P7/52C12R1/01
Inventor 陈银广李响王怀臣
Owner TONGJI UNIV
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