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Generation bacterium of heat-stable phytase

A technology for producing phytase and bacteria, which is applied in the field of microorganisms and can solve problems that hinder wide application

Inactive Publication Date: 2008-01-30
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, phytase has been used in actual production as a feed additive, but there are still technical problems hindering its wide application

Method used

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  • Generation bacterium of heat-stable phytase
  • Generation bacterium of heat-stable phytase

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Embodiment 1

[0035] 1. Isolation of strains

[0036] Water samples from the hot springs in Tengchong, Yunnan, China are directly coated on the primary screening medium plate and cultivated at 50°C for 3-5 days, observe the growth of the transparent circle, and select the strain with a large H / C ratio (H / C is the diameter of the transparent circle H and the colony) Diameter C ratio), select large and obvious colonies with transparent circles, separate and purify by streaking 2-3 times; after purification, put the strains into the liquid fermentation medium shake flask for fermentation, and ferment under the conditions of 37°C, 200, and rpm After 5 days, further screening was carried out, and finally the strain producing thermostable phytase was isolated and screened.

[0037] Media used

[0038] Primary screening medium: glucose 3.0g, NH 4 NO 3 0.5g, MgSO 4 0.003g, distilled water 100ml, calcium phytate 0.5g, agar 2g, pH5.5.

[0039] Liquid shake flask fermentation medium: peptone 1....

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Abstract

The invention relates to producing strains of a thermostable phytase. The classified nomenclature of the strains is bacillus subtilis with a preservation number of CGMCC No.2076. The phytase produced by the strains of the invention has a most suitable pH of about 5.5, when the pH is 2.2, the activity of remaining enzyme is 47.7 percent after the enzyme liquid is kept at room temperature for 2hr; the most suitable temperature is 60 DEG C, when the temperature is within the scope of 55 DEG C -65 DEG C, the activity of the enzyme changes little; if the enzyme is respectively kept for heat preservation at 70 DEG C for 60min, 120min and 180min, the activity of the remaining enzyme is 70 percent, 68 percent and 63 percent respectively; if the phytase is respectively kept for heat preservation at 80 DEG C for 60min, 120min and 180min, the activity of the remaining enzyme is 43 percent, 23 percent and 15 percent respectively; if the enzyme is respectively kept for heat preservation at 90 DEG C for 60min and 120min, loss of the activity of the enzyme is obvious with the activity of the remaining enzyme of 21 percent and 10 percent respectively; therefore, the enzyme has good temperature resistance. The strains of the invention not only meets the requirements of high temperature sterilization in feed process industry, but also meets the requirement of short-time of high temperature (73 DEG C-95 DEG C) in the granulation technique of grain feed. The thermostable phytase produced by the strains can be used as feed additive with excellent effect when is applied to actual production.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a heat-resistant phytase-producing bacterium. Background technique [0002] Phytic acid (phytate, Phytic acid, IP6) is also known as inositol hexaphosphate (phosphoric acid ester of the cyclicalcohol inositol, myo-inositol-hexakisphosphate), molecular formula C6H18O24P5. Phytic acid molecule contains 6 phosphate groups, which is an important storage form of phosphorus. About 60% to 80% of organic phosphorus in plants exists in phytic acid as a carrier. Phytic acid not only prevents phosphorus from being utilized by monogastric animals, but also as an anti-nutritional factor, phytic acid molecules can quickly combine with divalent and trivalent cations (such as Ca 2+ , Mg 2+ , Fe 3+ , Zn 2+ etc.) and protein to form an insoluble chelate, which hinders the digestion, absorption and utilization of other mineral elements, protein, starch, lipids, vitamins and other nutrien...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/31C12R1/125
Inventor 黄遵锡桂萍唐湘华
Owner YUNNAN NORMAL UNIV
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