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Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover

A technology of kelp leftovers and ganoderma strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of terminal food safety hazards, single variety of polysaccharide products, secondary pollution, etc., and achieve broadened utilization efficiency, shorten fermentation time, and reduce pollution

Inactive Publication Date: 2009-05-06
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the disadvantages in the prior art that kelp products are prepared by chemical methods, there are low yields, secondary pollution, potential hazards of acid or alkali to terminal food safety, and a single variety of polysaccharide products prepared, the present invention provides a Method for preparing polysaccharide by fermenting kelp leftovers with ganoderma lucidum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Weigh 140kg of kelp scraps after kelp hot water extraction of kelp juice, add 7000kg of water, after fully imbibition, add 1kg of cellulase, use 1M citric acid to adjust the pH value to 5.0, hydrolyze at 50°C for 10 hours, after the end of enzymatic hydrolysis Use 1M sodium bicarbonate solution to adjust to neutrality, control the temperature at 100°C for 10 minutes to inactivate the enzyme, use a desktop centrifuge at 4000 rpm, and centrifuge for 10 minutes to obtain sediment and kelp enzymatic hydrolysis solution. The sediment is dried and pulverized as feed. Take the supernatant of the kelp enzymatic hydrolysis solution and put it directly into the fermenter that has been sterilized, and then carry out the real sterilization. Enter the secondary Ganoderma lucidum strain, the secondary Ganoderma lucidum strain is 30 ℃, 160rpm liquid submerged secondary culture for 7 days, the inoculation amount is 8% of the kelp enzymatic hydrolysis supernatant, the pH value is contro...

Embodiment 2

[0019] Weigh 160kg of kelp scraps after soaking kelp juice in hot water, add 9600kg of water, after fully imbibed, add 1.5kg of cellulase, use 1M acetic acid to adjust the pH value to 6.0, hydrolyze at 60°C for 6 hours, after the enzymatic hydrolysis Adjust the solution to neutral with 1M sodium bicarbonate, control the temperature at 100°C for 10 minutes to inactivate the enzyme, and use a desktop centrifuge at 4000rpm to centrifuge for 10 minutes to obtain sediment and kelp enzymatic hydrolysis. The sediment is dried and pulverized as feed. Take the supernatant of the kelp enzymatic hydrolysis solution directly into the fermenter that has been sterilized, and then carry out the real sterilization. The temperature of both the sterilized and the real sterilized is 121°C, and the time is 20 minutes. After the temperature of the fermenter drops to 30°C Insert the second-grade ganoderma strains on the left and right sides. The second-grade ganoderma lucidum strains are formed by ...

Embodiment 3

[0021]Weigh 100kg of kelp scraps after soaking kelp juice in hot water, add 4000kg of water, add 1kg of cellulase after fully imbibed, use 1M acetic acid to adjust the pH value to 4.5, hydrolyze at 45°C for 12 hours, and use Adjust the 1M sodium bicarbonate solution to neutral, treat at 95°C for 15 minutes to inactivate the enzyme, use a desktop centrifuge at 4000rpm, and centrifuge for 10 minutes to obtain the sediment and kelp enzymatic solution. The sediment is dried and pulverized as feed, and the kelp enzyme is taken The supernatant of the solution is directly put into the fermentation tank that has been sterilized, and then the real sterilized. The temperature of both the sterilized and the real sterilized is 121°C, and the time is 20 minutes. When the temperature of the tank drops to about 30°C, it is connected to the second stage. Ganoderma lucidum strain, the second-level Ganoderma lucidum strain is obtained by submerged secondary culture at 30°C and 160rpm for a total...

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Abstract

The invention relates to a method for preparing polysaccharide by using lucid ganoderma strain to ferment kelp waste, which comprises the following steps: using the kelp waste as a raw material, killing enzyme and centrifugating the raw material after the raw material is subjected to enzymolysis of cellulose; putting supernatant into a fermentation tank, inoculating deeply fermented lucid ganoderma strain, and quickly fermenting the lucid ganoderma strain under aerobic condition to obtain fermentation liquor and lucid ganoderma mycelium after the filtration; depositing the fermentation liquor by ethanol to obtain composition of kelp polysaccharide and lucid ganoderma polysaccharide; and drying the lucid ganoderma mycelium, and extracting the lucid ganoderma polysaccharide. The method combines enzyme treatment and fermentation of the lucid ganoderma strain to treat the kelp waste, shortens fermentation time, obtains lucid ganoderma endoenzyme and endoenzyme polysaccharide as well as kelp polysaccharide at the same time, has reasonable and effective preparing technology, originates a novel process route, realizes comprehensive utilization of the kelp waste, reduces production cost, improves economic effect, and reduces pollution to environment at the same time.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a method for preparing polysaccharides by using ganoderma lucidum strains to ferment kelp leftovers. Background technique [0002] my country is a big ocean country, and its kelp output accounts for half of the world's total output, ranking first in the world. At present, my country has formed a system of seaweed chemical products mainly including alginate, mannitol and iodine. However, in terms of dry matter, the industrial utilization rate of kelp is only 30%, and about 2 / 3 of the kelp leftovers have not been utilized, which not only wastes a lot of natural resources, but also brings a series of environmental pollution problems. In addition, seaweed chemical production enterprises are mostly distributed in coastal areas, and a large amount of waste rich in organic matter and nutrient salts has become a potential chemical factor induced by eutrophication and red tide, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 孟秀梅刘昌衡唐聚德袁文鹏王小军夏雪奎张绵松胡炜孙永军
Owner SHANDONG HOMEY AQUATIC DEV
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