A temperature control apparatus applied to a nucleic acid amplification technique and a control method thereof

A temperature control device and nucleic acid technology, which are applied in the field of temperature control devices of nucleic acid amplification technology, can solve the problems of high production cost, complicated operation and low control precision, and achieve the effects of low production cost, simple operation and wide application.

Inactive Publication Date: 2017-08-04
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the current technical problems of high production cost, complicated operation and low control precision of the amplification instrument.

Method used

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  • A temperature control apparatus applied to a nucleic acid amplification technique and a control method thereof
  • A temperature control apparatus applied to a nucleic acid amplification technique and a control method thereof

Examples

Experimental program
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Embodiment Construction

[0024] Such as figure 2 As shown, a temperature control device applied to nucleic acid amplification technology is characterized in that the device includes a heat cover 1, a test tube 2, a temperature-variable metal block 3, a heat-conducting silicone grease 4, a semiconductor cooling sheet 5, a heat-dissipating metal sheet 6, Heat sink 7, fan 8, rubber base 9, full H-bridge drive module 10, controller 11, buttons 12, display 13, box 14 and temperature sensor 15;

[0025] The top of the test tube 2 is provided with a heat cover 1, and the lower part of the test tube is arranged in the temperature-variable metal block 3. The heat cover 1 and the temperature-variable metal block 3 are provided with a temperature sensor 15, and the bottom of the temperature-variable metal block 3 is provided with a semiconductor cooling chip 5, and a semiconductor A heat dissipation metal sheet 6 is provided below the cooling sheet 5, and thermal conductive silicone grease 4 is coated between t...

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Abstract

The invention discloses a temperature control apparatus applied to a nucleic acid amplification technique and a control method thereof. The apparatus comprises a hot cover, test tubes, a temperature changing metal block, thermally conductive silicone grease, a semiconductor refrigeration sheet, a heat radiation metal sheet, a heat radiation apparatus, draught fans, a rubber pedestal, a full H-bridge driving module, a controller, a button, a display screen, a casing body and a temperature sensor. The temperature control apparatus of the invention is low in production cost, simple to operate and broad in application. Both the PCR amplification technique in the early stage and an emergent LAMP or RPA amplification technique can be switched and set in a customized mode on the apparatus through a man-machine interaction interface.

Description

technical field [0001] The invention belongs to the field of industrial automation control, in particular to a temperature control device applied to nucleic acid amplification technology. Background technique [0002] Nucleic acid amplification is a common research tool in the field of life sciences. In vitro nucleic acid amplification using polymerase chain reaction (PCR) is a revolutionary technology developed since 1983. It has been widely used in modern agriculture and In the fields of medicine and food industry, especially in the process of human cognition of genes and genomes, in vitro nucleic acid amplification technology has made outstanding contributions. [0003] Because traditional PCR is always limited by many factors such as equipment, power and space. In recent years, constant temperature PCR technology has begun to rise quietly. In the past 10 years, several constant temperature nucleic acid amplification technologies have developed rapidly, such as SDA tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G05D23/32
CPCG05D23/32
Inventor 孔亚广常昊李文倩
Owner HANGZHOU DIANZI UNIV
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