PSA (Prostate Specific Antigen) high-sensitivity detection method based on nanotechnology

A detection method and nanotechnology technology are applied in the detection field of immunoenzyme-linked adsorption technology to achieve the effects of stable performance, low cost and easy analysis
CN102520189AInactive Publication Date: 2012-06-27CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2012-06-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

A PSA (Prostate Specific Antigen) high-sensitivity detection method based on nanotechnology is a novel biological high-sensitivity detection method which combines the magnetic bead probe technology, Au-nanoparticle double-mark probe technology, enzyme-linked immunosorbent assay technology and the nucleic acid amplification technology together. According to the detection method, an antibody corresponding to a protein to be detected (such as PSA) is cross-linked with a magnetic bead, and after the protein to be detected in a sample is captured, an Au-nanoparticle dual-mark probe marked by another antibody and nucleic acid is added to the captured protein. After magnetic separation and purification are carried out, nucleic acid undergoes release amplification, a visual result is obtained by means of agarose gel electrophoresis, and the grey level value scanning is subsequently performed. Compared with the conventional ELISA (Enzyme-Linked Immunosorbent Assay), the detection sensitivity of the detection method disclosed by the invention is increased by six orders of magnitude, and the PSA high-sensitivity detection method based on nanotechnology is fairly significant for the early diagnosis of caners and the detection of trace important substances, nucleic acids and proteins.
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Description

technical field

[0001] The present invention relates to a detection method of immunoenzyme-linked adsorption technology, in particular to a gold nanoparticle double-labeled probe-PCR (Polymerase Chain Reaction, polymerase chain reaction) that greatly improves the sensitivity of the detection method of immunoenzyme-linked adsorption technology. formula reaction) amplification detection method. Background technique

[0002] Immune response is an immunological phenomenon in which members of the immune system (antigens, immune molecules, immune cells, and immune tissues) depend on each other, influence each other and interact with each other. The immunoassay method established based on the specific reaction of antigen and antibody is a method that utilizes the highly specific interaction between antigen and antibody (ie, immune response) to realize the detection of antigens, antibodies, hormones, receptors and receptors in blood or urine samples. An analytical method for the id...

Claims

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