Loop-mediated isothermal amplification detection method and kit for H1N1 influenza A viruses

An influenza virus, ring-mediated constant temperature technology, applied in the biological field, can solve the problems of poor specificity, time-consuming and laborious, etc., and achieve the effect of rapid detection and high specificity
CN102108418AInactive Publication Date: 2011-06-29GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
0 Cites 15 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Publication Date
2011-06-29
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention relates to a method for detecting H1N1 influenza A viruses based on the LAMP (loop-mediated isothermal amplification of nucleic acid) technology, which comprises the following steps: extracting virus RNA (ribonucleic acid); carrying out reverse transcription and LAMP amplification; converting the LAMP amplification product into single-chain substances; and detecting the LAMP amplification product by using a nucleic acid nano-gold biosensor. The invention also provides a kit for detecting H1N1 influenza A viruses. By using the specially-designed LAMP inner primer to introduce an enzyme cutting site sequence segment into the amplification product in the LAMP amplification process, using the restriction endoenzyme TspR I to digest the amplification product and acquiring a large number of single-chain DNA (deoxyribonucleic acid) products with the length of 140bp or so by physical shock cooling, the combination of the LAMP technology based on the specific primer and the nucleic acid nano-gold biosensor with a specific probe ensures that the detection method and kit has high specificity and high detection speed.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for rapid detection of influenza A (H1N1) virus nucleic acid and a detection product. Background technique

[0002] Influenza, referred to as influenza, is an acute respiratory infectious disease caused by three types of influenza viruses: A, B, and C. Among them, the host of influenza A virus is the most extensive and the most harmful, and humans, poultry, and livestock are all hosts of influenza A virus. According to its surface structure (hemagglutinin H and neuraminidase N) and its genetic characteristics, influenza A virus can be divided into many subtypes. Up to now, influenza A virus has discovered 16 subtypes of hemagglutinin (H1-H16), neuraminidase has 9 subtypes (N1-N9).

[0003] In 2009, the influenza A (H1N1) influenza epidemic, which broke out from Mexico and spread to many countries around the world, was caused by a new variant of the influenza A (H1N1) virus, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More