Quick identification and separation method of affine nucleic acid molecule

Abstract

The invention discloses a method for quickly obtaining molecules with characteristics from a complicated nucleic acid library. The method comprises the following steps: firstly, respectively amplifying a single molecule of the library on a single micro-bead A by an emulsion nucleic acid amplification technique, so that only a nucleic acid molecule with a specific sequence is formed on each micro-bead A; coupling a target molecule on another micro-bead B (such as a magnetic bead) with different properties, mixing the two micro-beads together to combine, and then cleaning uncombined micro-bead A, and separating the micro-bead B with the target molecule, so that the micro-bead A combined with the target molecule is separated together; the nucleic acid molecule which is specifically combined with the target molecule on the micro-bead B on the micro-bead A is a required single sequence nucleic acid molecule. The method is fast and convenient; the single nucleic acid molecule with high affinity can be obtained without cloning and sequencing. The technique can be widely applied to screening of promoters, and screening and identifying of aptamers.

Description

technical field

[0001] The invention relates to an identification and separation method, in particular to a method for quickly identifying and isolating molecules with certain characteristics from a mixed nucleic acid molecule library. Background technique

[0002] Nucleic acid molecules, including DNA and RNA, play an extremely important role in living organisms. Some of the nucleic acid molecules can interact with other molecules, and through this interaction, information can be transmitted and specific functions can be performed. Such nucleic acid molecules capable of interacting with other molecules are not only ubiquitous in living organisms, but also can be screened in vitro to obtain nucleic acid molecules with specific functions. This in vitro screening technology is called SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology, which was independently invented by three laboratories in the United States in 1990.

[0003] Whether it is looking ...

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