Long life luminous nanometer bio probe for detecting pathogenic microorganism and preparing and detecting method thereof

Abstract

The invention discloses a long-life nano bioluminescent probe used for detecting pathogenic microorganisms, a preparation method and a detecting method thereof. Long-life luminous nano particles used for the nano probe are prepared; a capture probe DNA1 on a synthetic glass chip, a discriminating probe DNA2 connected on the long-life luminous nano particle and a target probe on the gene fragment of the pathogenic microorganism are designed to construct a detecting model which is similar to a sandwich structure. The invention has the advantage that the interference of source fluorescence and background signals can be effectively eliminated by utilizing the long-life luminous nano particles, and has the advantages of high sensitivity, stability, function of signal transformation and the like of nano technology. The detecting method has the advantages of simple and convenient operation, low cost, high sensitivity and specificity and good stability.

Description

technical field [0001] The invention relates to a long-life luminescent nano-biological probe for detecting pathogenic microorganisms, and also relates to a preparation and detection method of the probe. Background technique [0002] The existence of pathogenic microorganisms in the environment is a serious threat to human health. Whether in developing countries or developed countries, the proportion of biological hazards is greater than that of other factors such as chemical hazards. The detection and identification of bacterial pathogens in the environment is of increasing importance. [0003] Current methods for detecting pathogenic microorganisms include polymerase chain reaction (PCR, polymerase chain reaction), fluorescence analysis, fluorescence in situ hybridization (FISH, fluorescence in site hybridization) and gene chip (gene chip) methods. PCR reaction can be inhibited by some substances in nature, such as humic acid and fulvic acid. Similarly, during the proces...

AI Technical Summary

Problems solved by technology

For the detection of common pathogenic microorganisms in the environment, FISH method is one of the most widely used and most effective techniques, but the fluorescent probes in FISH method are easily interfered by the endogenous fluorescence background of the biological system and the background of the matrix used to fix microorganisms. Sensitivity improvement is limited when detecting

The technology of gene chip method is not very perfect, and there are still some key problems to be solved, such as: specificity, sample preparation, gene amplification, sensitivity, etc. There are still many problems, and the cost is high

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