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Biomarker used for early diagnoses of liver cancer and application thereof

A technology of biomarkers and markers, applied in the field of biomedicine

Inactive Publication Date: 2018-09-07
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

However, whether gut microbial markers can be used to distinguish early-stage HCC has not been reported

Method used

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  • Biomarker used for early diagnoses of liver cancer and application thereof
  • Biomarker used for early diagnoses of liver cancer and application thereof
  • Biomarker used for early diagnoses of liver cancer and application thereof

Examples

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Embodiment Construction

[0043] The present invention will be further described below in conjunction with the examples, but the protection content of the present invention is not limited to these examples.

[0044] The methods used in the following examples are conventional methods unless otherwise specified. The materials or reagents required in the following examples were obtained from open commercial channels unless otherwise specified.

[0045] We prospectively collected 486 stool samples from East China, Central China, and Northwest China. After a strict pathological diagnosis and exclusion process, 419 patient samples were finally included in the study, and 16S rRNAMiesq sequencing of intestinal microorganisms was completed. The included patients were randomly divided into a marker discovery period and a validation period. During the discovery period, 75 cases of early cirrhotic liver cancer, 40 cases of liver cirrhosis and 75 healthy controls described the intestinal microecological character...

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Abstract

The invention relates to a marker for intestinal microecology and application thereof, in particular to a biomarker used for early diagnoses of liver cancer. The biomarker used for early diagnoses ofliver cancer is composed of 34 kinds of genes shown as SEQ ID NO:1-34, and the genes are enriched in intestinal tracts. The invention also provides a detection reagent, and the reagent includes a primer used for detecting the 34 kinds of genes shown as SEQ ID NO:1-34. Feces of patients in groups are collected through a noninvasive method, and 16S rRNA Miseq sequencing of intestinal florae is conducted. During a discovery period of the biomarker, through a random forest law, microorganism gene markers with specificity of early liver cancer are identified among patients suffering from early cirrhosis and liver cancer, patients suffering from cirrhosis and healthy comparisons, an index of a probability of disease (POD) of liver cancer is created, and verification of the markers during the discovery period is achieved. During the verification period of the markers, the POD index of the patients during the verification period is calculated, and verification of the diagnosis value of the patients suffering from liver cancer during the verification period according to the POD index is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an intestinal microecological marker and its application. Background technique [0002] Liver cancer is the third leading cause of cancer-related death worldwide. In the United States, there are currently an estimated 29,200 new cases of liver cancer in men and 11,510 new cases in women. In China, due to the persistent infection of hepatitis B virus and the prevalence of liver cirrhosis, the incidence of liver cancer is more serious. There are 343,700 new liver cancer patients in men and 122,300 new liver cancer patients in women. Due to the lack of specific symptoms and effective early diagnostic markers in the early stage of liver cancer, most patients with liver cancer are diagnosed at an advanced stage, and the prognosis is poor (the overall mortality / incidence ratio is about 0.95). Therefore, novel diagnostic markers and novel therapeutic strategies are urg...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886
Inventor 任志刚李昂蒋建文郑树森鲁海峰崔光莹周琳陈新华
Owner ZHEJIANG UNIV
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