Psoriasis gene screening kit and detection method thereof

Abstract

The invention discloses a kit for screening a psoriasis gene. The kit comprises the following components: 0.5 ml of PCR (Polymerase Chain Reaction) Buffer, 0.4 ml of 25mM MgCl2, 0.1 ml of 25mM dNTP mix, 0.1 ml of 5U / mu LHotStar Taq, 1ml of PCR primer mix, 0.17 ml of 10x SAP Buffer, 0.3 ml of 1.7 U / mu l SAP enzyme, 0.94 ml of Extend primer Mix 1 or 2, 0.2 ml of iPLEX Buffer, 0.2 ml of iPLEX terminator, 0.041 ml of iPLEX enzyme, 80ml of Clean Resin resin, 80ml of ultrapure water, 0.5 pmol of a first round of amplification primer per strip, and 0.5 pmol of a second round of amplification primer per strip. Primers for the first-round amplification comprise an upstream primer and a downstream primer; the primer of the second round of amplification is a single-base extension primer. The kit is a reagent for in-vitro diagnosis, and the expression condition of the psoriasis susceptibility gene in a human serum or plasma sample is detected by applying an indirect method principle, so that early screening of psoriasis is realized, and a direction is provided for clinical disease prediction, differential diagnosis and layered treatment.

Description

technical field [0001] The invention relates to the technical field of psoriasis gene screening, in particular to a psoriasis gene screening kit and a detection method thereof. Background technique [0002] Psoriasis is a common, chronic, immune-mediated inflammatory skin disease with no clear etiology. Psoriasis types include plaque psoriasis, guttate psoriasis, and erythrodermic psoriasis. psoriasis and pustular psoriasis, the vast majority of which are chronic plaque psoriasis, with the classic clinical presentation of well-defined erythematous and pruritic erythematous plaques covered by silvery scales. The incidence of psoriasis is extremely high in developed countries in the West and relatively low in developing countries in Asia, Africa and the Pacific, but is increasing. The number of psoriasis patients in my country, the age-standard incidence rate, the incidence rate and the age-standard incidence rate all show a continuous growth trend. Psoriasis mostly occurs i...

AI Technical Summary

Problems solved by technology

Despite the enormous contribution of GWAS to psoriasis research, even the most important loci in the genome have small effects in GWAS, and together these important results explain only a small fraction of the predicted genetic variation , which is known as the missing heritability problem

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