Novel coronavirus dominant epitope fusion protein, diagnostic reagent and application

Abstract

The invention relates to novel coronavirus dominant epitope fusion protein, diagnostic reagent and application, which belongs to virus diagnostic reagent. The novel coronavirus dominant epitope fusion protein of the present invention comprises N protein dominant epitope peptide and S protein dominant epitope peptide, E protein dominant epitope peptide connected by linking arm, and said N protein dominant epitope peptide comprises dominant epitope peptide One or more of the dominant epitope peptide N1, dominant epitope peptide N2, dominant epitope peptide N3 and dominant epitope peptide N4. The novel coronavirus dominant epitope fusion protein of the present invention has higher specificity, higher sensitivity (higher positive detection rate), biocompatibility, high solubility, good stability, and is more conducive to the preparation of corresponding detection reagents; The detection reagent prepared based on the fusion protein of the present invention has high sensitivity and good specificity, and can realize early screening of novel coronavirus.

Description

technical field [0001] The invention relates to the technical field of virus diagnostic reagents, in particular to novel coronavirus dominant epitope fusion proteins, diagnostic reagents and applications. Background technique [0002] Coronaviruses are a large family of viruses known to cause more serious illnesses such as colds, Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The new coronavirus is a new strain of coronavirus that has never been found in humans before, and 2019-nCoV is the seventh type of coronavirus isolated from humans. According to relevant disease monitoring, many cases of viral pneumonia were found, all of which were diagnosed as viral pneumonia / lung infection. [0003] After the new coronavirus infects the human body, there is generally an incubation period of 3 to 14 days (in some cases, up to 24 days). During the incubation period, there may be no clinical symptoms and it is difficult to detect. Current eviden...

AI Technical Summary

Problems solved by technology

After the virus infects the human body, it releases a large amount of spike protein (Spike protein, S protein) and nucleoprotein (Nucleoprotein, N protein) to stimulate the body to produce an immune response and produce antibodies against these two proteins. The produced diagnostic reagents will cause incomplete capture, and the sensitivity of missed detection is not high.

The use of two kinds of protein mixed coating will make the production process complicated, and the batch-to-batch control is difficult

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