LAMP-based (loop-mediated isothermal amplification-based) visual fluorogenic and chromogenic genetic testing method for microorganisms

A gene detection and microbial technology, applied in biochemical equipment and methods, microbial measurement/inspection, fluorescence/phosphorescence, etc., can solve the problems of poor specificity, low sensitivity, time-consuming and laborious, etc., and achieve fast and repeatable detection Highly specific and specific effects
CN102586438AInactive Publication Date: 2012-07-18WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an LAMP-based visual fluorogenic and chromogenic genetic testing method for microorganisms, which relates to a genetic testing method for microorganisms. The testing method includes the following steps: (1) buffered peptone water (BPW) is used for culturing a sample to be tested according to a national standard method for 4 hours; (2) a water boiling method is used for extracting DNA (deoxyribonucleic acid) from the sample to be tested; (3) the DNA is added into an LAMP reaction system, and the temperature of 65 DEG C is kept for 1 hour; (4) by means of comparison with a control group, the result of the sample to be tested is observed with naked eyes, and qualitative analysis is carried out on the sample. Compared with the conventional culture method, the genetic testing method has the advantages that: testing is rapid, only taking 5 hours, and plus the extraction of the sample DNA, testing takes less than 6 hours in total; the specificity is good, and the sensitivity is high; the steps are simple, and the repeatability is high; and a plurality of samples can be tested at the same time. The genetic testing method can qualitatively test microorganisms, and can take the place of the conventional culture method and the serological testing method which are used up to now.
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Description

technical field

[0001] The invention relates to a microorganism gene detection method, in particular to a LAMP-based microorganism visual fluorescence color gene detection method, which is suitable for the qualitative detection of microorganisms. Background technique

[0002] Microorganisms are a large group of organisms including bacteria, viruses, fungi, and some small protozoa and microscopic algae. Microorganisms are tiny, but they are closely related to human life. They cover many beneficial and harmful species, and are widely involved in many fields such as health, food, medicine, industry and agriculture, and environmental protection. One of the most important impacts of microorganisms on humans is the prevalence of infectious diseases, and 50% of human diseases are caused by viruses. Food contamination is a serious threat to human health, and food-borne pathogens are pathogens that cause bacterial food poisoning. Therefore, microbial detection is of great significa...

Claims

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