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Mental disorder biomarker and application thereof

A technology for biomarkers and mental disorders, applied in the fields of application, biochemical equipment and methods, microbiological determination/testing, etc., can solve the problem that the impact mechanism is not very clear

Active Publication Date: 2016-05-04
金锋 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the specific impact mechanism is not very clear, and the mechanism by which the metabolites and functional genes of the intestinal flora affect the host still needs further research

Method used

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  • Mental disorder biomarker and application thereof
  • Mental disorder biomarker and application thereof
  • Mental disorder biomarker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In the example, we recruited 31 individuals with mental disorders and 32 healthy controls. We took 62 individuals with mental disorders and healthy controls as the training sample set for the model, and collected 10 individuals from the mental disorder group and 10 healthy controls as the validation sample set. The potential biomarkers of significant differences between the two groups were found by comparative analysis of the functional genetic composition characteristics of gut microbes in fecal samples from the model learning sample set. Subsequently, the above-mentioned potential biomarkers are verified through the verification sample set, so as to screen out the final biomarkers. High-throughput chip detection and bioinformatics analysis showed that there were different kinds of representative functional genes between the two groups in the number of detected genes, diversity and different metabolic pathways. Example 1: Sample Collection Detection and DNA Extraction...

Embodiment 2

[0032] Example 2: Detection of fecal DNA using a functional gene detection chip

[0033] Qualified DNA samples were vacuum freeze-dried and then sent to AG Company for gene chip testing. The microbial gene chip of AG company was used for detection. Wherein, sample preparation, hybridization, reading, etc. are carried out referring to the instructions of the chip. The microbial gene chip contains 36,802 probes, corresponding to 50,007 protein coding sequences of 139 key metabolic pathway functional gene families, which can detect the functional genes of human microorganisms at the species / strain level (Tu, Q., Z. He, Y. Li, Y. Chen, Y. Deng, L. Lin, et al. (2014): Development of HuMiChip for Functional Profiling of Human Microbiomes. PLOSONE. 9(3): e90546.). The hybridization results were scanned and analyzed using NimbleScan (NimbleGen, Madison, WI, USA), and the raw data were input into the online analysis platform (http: / / ieg.ou.edu / microarray / ), and processed according to...

Embodiment 3

[0034] Embodiment 3: Basic processing of chip data

[0035] After obtaining the chip data of 63 samples, the original data needs to be normalized, and each detected signal value is divided by the average signal intensity of the chip. Then perform quality control on it, and the quality control is carried out according to the following standards: the minimum signal strength is set to 1000, and the signal-to-noise ratio (SNR, SignaltoNoiseRatio) is set to 2. After the original data is processed, Cleandata is obtained, and the online analysis tool (http: / / ieg2.ou.edu / NimbleGen) is used to perform operations such as de-redundancy to screen high-quality data. An average of 27,968 gene signals were detected per sample, and an average of 20,532 high-quality gene signals were obtained per sample.

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Abstract

The invention discloses a mental disorder biomarker and application thereof. Microorganism gene chip hybridization research is conducted on intestinal flora microbial genomes in fecal samples of a mental disorder group and fecal samples of a healthy control group to describe characteristics of fecal microflorae and functional genes. By means of difference testing, discriminant analysis and machine learning, fifteen functional genes are recognized finally and can serve as biomarkers to highly accurately distinguish the mental disorder group. The biomarker is selected from at least ten genes in the fifteen functional genes. The fifteen functional genes have obvious differences in mental disorder people and the healthy people. The invention further discloses a drug which is capable of changing the number or expression of genes and is used for treating mental disorder, a method for producing or screening the drug for treating mental disorder and a kit for detecting mental disorder.

Description

technical field [0001] The invention relates to the fields of biotechnology, disease diagnosis and biomedicine, in particular to biomarkers of mental disorders and applications thereof. Background technique [0002] Mental disorders are a syndrome of important cognitive, emotional, behavioral and other psychological disorders, which can cause individual disorders, pain, and even disability. Common mental disorders are mainly manifested as dementia, depression, anxiety, mania, delusions, hallucinations, affective disorders, strange behavior, and split personality. It is estimated that about one in four people will experience at least one mental disorder in a year, and about 450 million people worldwide suffer from mental health effects. According to statistics in 2009, the global annual cost of mental disorders exceeds 2.5 trillion US dollars, and by 2030 it will exceed 6 trillion US dollars. It has become an important economic and social burden for individuals, families an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/52C12N15/53C12N15/54C12N15/61A23L33/00A61K45/00A61P25/18
CPCA61K45/00C12N9/00C12Q1/6883C12Q2600/158
Inventor 金锋段云峰
Owner 金锋
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