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Application of asparagus polysaccharide in the regulation of intestinal flora

A technology of aspartame polysaccharide and intestinal flora, which is applied in medical preparations containing active ingredients, drug combinations, organic active ingredients, etc. The effect of generating, reducing the ratio

Active Publication Date: 2021-11-12
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many structural characteristics and pharmacological effects of polysaccharides purified from Asparagus have not yet been clarified.

Method used

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  • Application of asparagus polysaccharide in the regulation of intestinal flora
  • Application of asparagus polysaccharide in the regulation of intestinal flora
  • Application of asparagus polysaccharide in the regulation of intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the extraction of aspartame polysaccharide

[0047] 1.1 Extraction of asparagus crude polysaccharide

[0048] Take 1.78 kg of dried Radix Asparagus, add ethanol to make it submerge the medicinal material, soak overnight to degrease. After all the ethanol was volatilized, 20 times the mass of pure water was added, heated to 100°C for continuous reflux extraction three times, each time for 6 hours, the residue was removed by gauze filtration, and the extract was obtained by combining. Concentrate it to 20% of the total amount by rotary evaporation, add absolute ethanol until the volume fraction concentration of the concentrated filtrate is 80°C, and place it at 4°C overnight; collect the solution in the bag and add it to absolute ethanol to make the final concentration of absolute ethanol Change to 80%, control the temperature at 4°C, let it stand overnight, and fully carry out alcohol precipitation. Discard the supernatant, add water to dissolve the resid...

Embodiment 2

[0059] Embodiment 2 Asparagus polysaccharide structure analysis

[0060] In this experiment, color reaction, infrared spectroscopy, high performance liquid chromatography (HPGPC), chemical derivatization combined with gas chromatography-mass spectrometry and nuclear magnetic resonance were used to study the structural characteristics of the isolated and purified polysaccharides. Pullulan series standard products used, CH 3 COONH 4 , phenol, concentrated sulfuric acid, NaOH, m-hydroxybiphenyl, α-D-galactose, α-D-galacturonic acid, m-hydroxybiphenyl, D-glucose, fructose, ammonia, acetic anhydride, 1-methylimidazole , anhydrous sodium sulfate, chloroform, methyl iodide, D 2 O. High-temperature-resistant stoppered glass reaction tubes, high-efficiency gel chromatography, infrared spectrometer, rotary evaporator, GC-MS instrument, nuclear magnetic resonance spectrometer, etc. were purchased from public channels in the market.

[0061] 2.1 Homogeneity analysis and molecular weigh...

Embodiment 3

[0093] Example 3 Regulatory Effect of Asparagus Polysaccharide on Intestinal Flora

[0094] In order to further study the application of the extracted aspartame polysaccharide in the regulation of intestinal flora, the present invention reflects the effect of aspartame polysaccharide by studying the reaction between aspartame polysaccharide and fecal inoculum and detecting various parameters of the product. In this part of the study, bile salts, hemin, yeast extract, peptone, resazurin, L-cysteine ​​hydrochloride monohydrate, vitamin K1 and short-chain fatty acid reference substances (acetic acid, propionic acid, iso Butyric acid, n-butyric acid, isovaleric acid and n-valeric acid) were purchased from Sigma-Aldrich Company. Inulin (molecular weight: 285.303Da) and 2-ethylbutyric acid of dahlia tubers were purchased from Shanghai Merrill Chemical Technology Co., Ltd.

[0095] 3.1 Fermentation

[0096] Fresh fecal inoculum from three healthy donors who had not taken any antibi...

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Abstract

The present invention provides a polysaccharide aspartame, including Fru and Glc, wherein Fru accounts for 93.3%, and Glc accounts for 6.7%; does not contain uronic acid and protein, and is composed of (2→1)-Fruf and t-Glcp Inulin type fructan, the relative molecular weight is 2.69×10 3 ; by taking asparagus and adding ethanol to degrease, then extracting with hot water reflux to obtain the extract, concentrating the extract and then ethanol precipitation, and obtaining the asparagus crude polysaccharide after dialysis of the obtained precipitate; After purification by agarose gel chromatography and Sephadex gel chromatography, aspartame is obtained. Aspartame polysaccharide can be used to regulate the intestinal flora, by adjusting the pH value of the intestinal lumen, it can promote the production of short-chain fatty acids, thereby having a corresponding beneficial effect on the intestinal tract. In addition, aspartame polysaccharide can also promote the beneficial bacteria Bacteroides and Megamonas increased, Haemophilus was suppressed, and the ratio of Bacteroides to Prevotella was decreased.

Description

technical field [0001] The invention relates to the application of aspartame polysaccharide in the regulation of intestinal flora. Background technique [0002] Gut microbiota is a complex and highly dynamic microbial community. As the largest and most complex micro-ecosystem in the human body, gut microbes and their metabolites can not only regulate human health, but also play a role in the relationship between diet and host. important bridging role. Studies have found that intestinal microorganisms basically belong to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Verrucomicrobia in terms of phylogenetic status. ), Fusobacteria (Fusobacteria) 6 gates, of which Bacteroidetes and Firmicutes are the main dominant bacterial groups, accounting for more than 90% of the entire phylogenetic types. Gut microbes themselves and their metabolites not only regulate human health, but also serve as an important bridge between diet and host. Dysregulation of the gut microbi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/733A61P1/00
CPCA61K31/733A61P1/00C08B37/0003C08B37/0054
Inventor 董彩霞许军孙启立朱利霞
Owner TIANJIN MEDICAL UNIV
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