Application of enteric microorganism as marker of bronchial pulmonary dysplasia of premature infant

A technology for intestinal microbes and dysplasia, applied in the biological field, can solve problems such as the inability to obtain intestinal bacterial colonization, intestinal microecological imbalance, and delayed colonization of intestinal flora

Active Publication Date: 2021-05-25
SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]Compared with normal full-term neonates, premature infants cannot obtain the natural growth status due to immature gastrointestinal tract development and exposure to environments such as hospitals after birth. Colonization of intestinal bacteria, delayed colonization of intestina...

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  • Application of enteric microorganism as marker of bronchial pulmonary dysplasia of premature infant
  • Application of enteric microorganism as marker of bronchial pulmonary dysplasia of premature infant
  • Application of enteric microorganism as marker of bronchial pulmonary dysplasia of premature infant

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Embodiment

[0032] The invention discloses the application of Klebsiella (Klebsiella), Prevotella (Prevotella) and Streptococcus (Streptococcus) in preparing microbial markers for bronchopulmonary dysplasia in premature infants; Klebsiella, Prevotella, and Streptococcus as microbial markers for bronchopulmonary dysplasia in preterm infants; in particular, Klebsiella, Prevotella Application of genera (Prevotella), Streptococcus (Streptococcus) in the preparation of microbial markers of early bronchopulmonary dysplasia in preterm infants, the early stage is from birth to 7 days. Through the detection of Klebsiella, Prevotella, and Streptococcus, early identification of high-risk infants with BPD, early intervention, and reduction of the occurrence of BPD, especially moderate / severe BPD rate and improve the quality of life of premature infants.

[0033] In order to verify the accuracy and specificity of using Klebsiella, Prevotella, and Streptococcus as microbial markers for bronchopulmonar...

Embodiment 2

[0128] Example 2 The system for assisting the diagnosis of bronchopulmonary dysplasia in preterm infants by using the intestinal microorganisms of the present invention includes a sequencing module to sequence the isolated intestinal flora nucleic acid samples (fecal DNA) to obtain sequencing results; the abundance calculation module, According to the sequencing results, the relative abundance of the microbial markers in the intestinal flora is detected to obtain a relative abundance value; the comparison module compares the obtained relative abundance value of the microbial markers with a set value. The set value is the relative abundance value of microbial markers obtained from the normal group (non-BPD group).

[0129] The application method of the invention is simple and does not require complicated data calculation. Stool samples were collected from 6 cases of premature infants who were clinically diagnosed as BPD on the 7th day of birth and 2 cases on the 4th day of ...

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Abstract

The invention discloses an application of an enteric microorganism as a marker of bronchial pulmonary dysplasia of a premature infant. The enteric microorganism is one or more of Klebsiella, Prevotella and Streptococcus. The application comprises the following steps: sequencing a separated intestinal flora nucleic acid sample to obtain a sequencing result, detecting the relative abundance of the intestinal microorganisms in the intestinal flora according to the sequencing result to obtain a relative abundance value, and comparing the obtained relative abundance value of the intestinal microorganisms with a set value, and taking the obtained result as intermediate information to assist in diagnosing the premature bronchial pulmonary dysplasia. The premature infant excrement sample 16SrDNA gene is sequenced through a high-throughput sequencing technology, the relationship between intestinal flora and premature infant BPD occurrence is discussed for the first time, and a reliable basis is provided for prevention and treatment of BPD.

Description

technical field [0001] The invention belongs to biotechnology, and specifically relates to the application of intestinal microorganisms as markers for bronchopulmonary dysplasia in premature infants. Background technique [0002] The "gut microbiota" is an umbrella term for the diverse microbial communities that colonize the host's gastrointestinal tract, including bacteria, viruses, fungi, and prokaryotes. More and more research shows that the gut microbiota is the key to maintaining health. With the development of 16SrDNA high-throughput sequencing technology, researchers have begun to focus on the research on intestinal flora and human health. Studies have found that intestinal flora play an important role in the metabolism, immune regulation and inflammatory response of diseases, and then the concepts of gut-brain axis and gut-liver axis were proposed. The lung and the intestine share a common embryonic origin and mucosal immune system. They are hollow organs that dire...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/06C12Q1/14C12Q1/10C12R1/22C12R1/46C12R1/01
CPCC12Q1/689C12Q1/06C12Q1/14C12Q1/10G01N2333/26G01N2333/315G01N2333/195G01N2800/12G01N2800/38Y02A50/30
Inventor 朱雪萍朱晓黎范佳英李静兰吴志新王华伟陈小倩俞贇
Owner SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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