Application of enteric microorganism as marker of bronchial pulmonary dysplasia of premature infant

Abstract

The invention discloses an application of an enteric microorganism as a marker of bronchial pulmonary dysplasia of a premature infant. The enteric microorganism is one or more of Klebsiella, Prevotella and Streptococcus. The application comprises the following steps: sequencing a separated intestinal flora nucleic acid sample to obtain a sequencing result, detecting the relative abundance of the intestinal microorganisms in the intestinal flora according to the sequencing result to obtain a relative abundance value, and comparing the obtained relative abundance value of the intestinal microorganisms with a set value, and taking the obtained result as intermediate information to assist in diagnosing the premature bronchial pulmonary dysplasia. The premature infant excrement sample 16SrDNA gene is sequenced through a high-throughput sequencing technology, the relationship between intestinal flora and premature infant BPD occurrence is discussed for the first time, and a reliable basis is provided for prevention and treatment of BPD.

Description

technical field [0001] The invention belongs to biotechnology, and specifically relates to the application of intestinal microorganisms as markers for bronchopulmonary dysplasia in premature infants. Background technique [0002] The "gut microbiota" is an umbrella term for the diverse microbial communities that colonize the host's gastrointestinal tract, including bacteria, viruses, fungi, and prokaryotes. More and more research shows that the gut microbiota is the key to maintaining health. With the development of 16SrDNA high-throughput sequencing technology, researchers have begun to focus on the research on intestinal flora and human health. Studies have found that intestinal flora play an important role in the metabolism, immune regulation and inflammatory response of diseases, and then the concepts of gut-brain axis and gut-liver axis were proposed. The lung and the intestine share a common embryonic origin and mucosal immune system. They are hollow organs that dire...

AI Technical Summary

Problems solved by technology

[0006]Compared with normal full-term neonates, premature infants cannot obtain the natural growth status due to immature gastrointestinal tract development and exposure to environments such as hospitals after birth. Colonization of intestinal bacteria, delayed colonization of intestinal flora, more prone to intestinal dysbiosis

BPD is common in premature infants, but due to many factors affecting the colonization of intestinal flora in premature infants and the difficulty of collecting specimens, there is still no research on the relationship between BPD and intestinal flora

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