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Rooting method outside tissue culture seedling bottle of medicinal plant monochasma sheareri

A technology of medicinal plants and exogenous roots, which is applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of immature tissue culture and rapid propagation technology, and achieve the convenience of factory seedling production and streamline the cultivation steps , The effect of convenient material collection

Active Publication Date: 2020-03-06
中国林业科学研究院亚热带林业实验中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the research and practice on the tissue culture of velvet antler shows that its tissue culture and rapid propagation technology is still immature, and a large number of aerial roots distributed on the surface of the medium are induced in the bottle, and there has been no report of transplanting and surviving.

Method used

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  • Rooting method outside tissue culture seedling bottle of medicinal plant monochasma sheareri
  • Rooting method outside tissue culture seedling bottle of medicinal plant monochasma sheareri
  • Rooting method outside tissue culture seedling bottle of medicinal plant monochasma sheareri

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Selection of bottle seedlings: choose cluster buds of velvet antler tissue cultured seedlings that grow well, the leaves are covered with wool, and the color is silvery white ( figure 1 ), for strong seedling cultivation;

[0055] (2) Cultivation of strong seedlings: Cut off the cluster buds of tissue cultured seedlings by individual plants, select single buds with terminal buds and seedling height of 2cm, and transfer them to the strong seedling medium. The strong seedling medium contains the following components: macroelements: KNO 3 :1200mg / L; NH 4 NO 3 :1650mg / L; Ca(NO 3 ) 2 4H 2 O:400mg / L; KH 2 PO 4 :170mg / L; MgSO 4 ·7H 2 O:370mg / L; CaCl 2 ·7H 2 O:240mg / L;

[0056] Organic ingredients: inositol: 200mg / L; glycine: 2mg / L; thiamine hydrochloride: 0.4mg / L; pyridoxine hydrochloride: 0.5mg / L; niacin: 0.5mg / L; other ingredients are the same as MS;

[0057] The medium formula is: improved MS + BA0.3mg / L + IAA0.5mg / L + GA0.2mg / L + sucrose 30g / L + agaric 1.8g...

Embodiment 2

[0063] (1) Selection of bottle seedlings: choose cluster buds of velvet antler tissue cultured seedlings that grow well, the leaves are covered with wool, and the color is silvery white ( figure 1 ), for strong seedling cultivation;

[0064] (2) Cultivation of strong seedlings: Cut off the cluster buds of tissue cultured seedlings by individual plants, select single buds with terminal buds and seedling height of 2cm, and transfer them to the strong seedling medium. The strong seedling medium contains the following components: macroelements: KNO 3 :1200mg / L; NH 4 NO 3 :1650mg / L; Ca(NO 3 ) 2 4H 2 O:400mg / L; KH 2 PO 4 :170mg / L; MgSO 4 ·7H 2 O:370mg / L; CaCl 2 ·7H 2 O:240mg / L;

[0065] Organic ingredients: inositol: 200mg / L; glycine: 2mg / L; thiamine hydrochloride: 0.4mg / L; pyridoxine hydrochloride: 0.5mg / L; niacin: 0.5mg / L; other ingredients are the same as MS;

[0066] The medium formula is: improved MS + BA0.3mg / L + IAA1.0mg / L + GA0.4mg / L + sucrose 30g / L + agaric 2.0g...

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Abstract

The invention belongs to the technical field of herbal medicinal plant tissue culture, in particular to a rooting method outside a tissue culture seedling bottle of monochasma sheareri. The method mainly solves the technical problems of long rooting period in the tissue culture seedling bottle, more induced aerial roots, low transplanting survival rate and the like, and includes the following steps: (1) selecting bottle seedlings; (2) strong seedling cultivation; (3) hardening seedlings; (4) transplanting tissue culture seedlings; and (5) post-planting management. The method establishes an effective rooting technology outside the tissue culture seedling bottle of monochasma sheareri and optimizes the tissue culture and rapid propagation system. According to the technology, rooting is not required in a bottle, the seedlings are directly transplanted after hormone treatment, two weeks later, new roots can be seen at the base of the seedlings; after 30 days, the number of roots reaches 5-10, and the root length is 0.5-1.5cm; and in about two months, robust rooted seedlings of monochasma sheareri with a seedling height of about 6-10cm can be obtained. Compared with the traditional rooting in a bottle, the seedling raising period is shortened by one month, and the transplanting survival rate is higher than 85%; and by adopting the technology of the method, the rooting seedling quality is obviously improved, and industrial production can be carried out.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture of velvet velvet, in particular to a method for rooting out of a bottle of the tissue culture seedling of the medicinal plant velvet velvet. Background technique [0002] Antler grass is a perennial semi-parasitic small herb of the family Scrophulariaceae, and it is one of the main components of Yanning Granules, a protected variety of traditional Chinese medicine in my country. It is mainly distributed in Jiangxi, Hunan, Fujian, Zhejiang, Jiangsu and other places in southeastern my country. It grows in the weeds on the sunny side of the hillside or under the masson pine forest. It has been reported in the literature that velvet antler contains a variety of secondary metabolites, and the isolated phenylethanol glycosides and flavonoids all have certain anti-complement activities, have antibacterial, anti-inflammatory and anti-viral effects, and are commonly used clinically. It is an ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李峰卿曾满生刘素贞
Owner 中国林业科学研究院亚热带林业实验中心
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