Colloidal gold detection card and preparation method thereof, and hormone residue detection method

A colloidal gold and detection card technology, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of low detection efficiency

Inactive Publication Date: 2019-04-16
SHENZHEN BAOANKANG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the existing colloidal gold detection cards can only detect a single item, that is, the detection of androgen (methyltestosterone) and the detection of estrogen (diethylstilbestrol) should be detected separately, and the detection efficiency is low

Method used

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  • Colloidal gold detection card and preparation method thereof, and hormone residue detection method
  • Colloidal gold detection card and preparation method thereof, and hormone residue detection method
  • Colloidal gold detection card and preparation method thereof, and hormone residue detection method

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Embodiment Construction

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0043] In addition, the technical solutions of the various embodiments of the present invention can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered as a combination of technical solutions. Does not exist, nor is it within the scope of protection required by the present invention.

[0044] The pre...

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Abstract

The invention discloses a colloidal gold detection card and a preparation method thereof, and a hormone residue detection method using the colloidal gold detection card. The colloidal gold detection card comprises a supporting plate, a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the colloidal gold combination pad, thenitrocellulose membrane and the water absorption pad are sequentially arranged on the plate surface of the supporting plate in the direction from the first end to the second end of the detection card,and the nitrocellulose membrane is provided with a detection line and a quality control line; and the colloidal gold combination pad is coated with an anti-methyltestosterone monoclonal antibody andan anti-diethylstilbestrol monoclonal antibody which are marked by colloidal gold. According to the technical scheme, the residues of the androgen and the estrogen in the aquatic products can be detected at the same time, and therefore the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a colloidal gold detection card, a preparation method thereof, and a hormone residue detection method using the colloidal gold detection card. Background technique [0002] In the aquaculture industry, male hormones (such as methyltestosterone) and female hormones (such as diethylstilbestrol) are usually used as specific drugs for seed development and sex control. However, the residue of these hormones in aquatic products seriously threatens human health, and the harm to human body is chronic, long-term and cumulative. In severe cases, it can also cause neonatal deformities and affect the normal growth and development of children. [0003] At present, the colloidal gold detection card is widely used in the detection of hormone residues in aquatic products due to its advantages of simplicity, speed, effectiveness, and high sensitivity. However, most of the existing colloi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/577G01N33/558
CPCG01N33/558G01N33/577G01N33/74
Inventor 黄荫翔
Owner SHENZHEN BAOANKANG BIOTECH
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