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Method for measuring sex hormone residue in cosmetics

A technology for cosmetics and sex hormones is applied in the field of determining the residual amount of sex hormones in cosmetics to achieve the effect of a simple method

Inactive Publication Date: 2019-01-11
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that there is no relevant report on the method of high performance liquid chromatography-mass spectrometry for the determination of sex hormone residues in cosmetics

Method used

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  • Method for measuring sex hormone residue in cosmetics

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Experimental program
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Embodiment 1

[0032] S1, sample pretreatment

[0033] Add 1g cosmetic sample to 2mL saturated potassium chloride solution and ultrasonically disperse, then add 0.4mL potassium ferrocyanide solution to the solution and ultrasonically disperse evenly, then add 0.4mL zinc acetate solution and ultrasonically disperse evenly, finally Centrifuge at a speed of 9000r / min for 5min to obtain the supernatant;

[0034] S2, sample extraction:

[0035] Aqueous agents, emulsions, and essence cosmetics: add 0.5g of the pretreated supernatant of the cosmetic sample to 5mL of acetonitrile, vortex and oscillate fully on a vortex mixer to mix evenly, and then add 20mL with a mass fraction of 50% acetonitrile solution, ultrasonic extraction for 20min, let stand to room temperature, then centrifuge at 12000r / min for 10min, take the supernatant and filter it with a 0.22μm filter membrane, take the filtrate as the test solution for later use;

[0036] Cream cosmetics: Add 0.5g of the pretreated supernatant of th...

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Abstract

The invention provides a method for measuring sex hormone residue in cosmetics. An analysis method for simultaneously measuring pregnendione, testosterone propionate, medroxyprogesterone acetate, norgestrelm, hydroxyprogesterone acetate and methyltestosterone residue in the cosmetics through high-performance liquid chromatography-tandem mass spectrum is established. The method comprises the following steps: firstly pretreating cosmetic samples of different preparations, and taking acetonitrile and n-hexane as extraction solvents, ultrasonically extracting for 20min, and centrifuging a sample extracting solution for 10min in the speed of 12000r / min; selecting the acetonitrile and water as moving phases to perform isogradient elution, wherein the flow velocity is 0.3mL / min, and the column temperature is 25 DEG C, and performing separation by adopting an octadecyl silane chemically bonded silica chromatographic column. A result shows that a linear regression equation correlation coefficient R of each of six sex hormones is 0.9993-0.99989, the recycling rate is between 97.6%-99.5%, and the accuracy is between 0.5%-3.1%. The method provided by the invention is simple, sensitive, fast, reliable, good in reproducibility, and suitable for content analysis detection of the hormone addition of sex hormones in the cosmetics.

Description

technical field [0001] The invention relates to a method for measuring cosmetics, in particular to a method for measuring residual sex hormones in cosmetics. Background technique [0002] Sex hormones refer to a class of steroid hormones that are synthesized by animal gonads, placenta and adrenal cortex reticular zone and can promote the maturation of sexual organs, mainly including estrogen, androgen and progesterone, which directly act on the skin and have the function of eliminating Wrinkles, increase skin elasticity. In recent years, with the improvement of people's consumption level of cosmetics, unscrupulous merchants often add sex hormones to various cosmetics. The use of the above cosmetics can eliminate wrinkles and increase skin elasticity in a short period of time, but long-term use will cause skin damage. Atrophy, induced breast cancer, ovarian cancer and other diseases. Therefore, how to quickly and accurately determine the residual amount of sex hormones in c...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 王敏郭立群
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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