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Colloidal gold test strip for detecting methyltestosterone residue

A technology of colloidal gold test paper and methyl testosterone, which is applied in the field of immunochemical rapid detection of veterinary drug residues, can solve the problems of inability to realize rapid detection of large batches of samples and high dependence on equipment, and achieve short detection time, high sensitivity and simple operation Effect

Inactive Publication Date: 2017-01-11
北京安达兴业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the existing method for detecting methyltestosterone, which are highly dependent on equipment and cannot realize rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, fast detection speed, and low cost. Low-cost test strips that are not limited by detection equipment to achieve rapid detection and on-site monitoring of large quantities of methyltestosterone samples

Method used

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  • Colloidal gold test strip for detecting methyltestosterone residue
  • Colloidal gold test strip for detecting methyltestosterone residue
  • Colloidal gold test strip for detecting methyltestosterone residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 detects the preparation of the colloidal gold test paper strip that methyltestosterone remains

[0034] The preparation method of this test strip mainly comprises the following steps:

[0035] 1) Prepare a conjugate release pad coated with methyltestosterone monoclonal antibody-colloidal gold marker;

[0036] 2) Prepare a reaction membrane with a detection line coated with a methyltestosterone hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;

[0037] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorbent pad, the water absorbent pad and the backing into a test strip.

[0038] The following step-by-step detailed description:

[0039] 1. Synthesis and identification of methyltestosterone hapten-carrier protein conjugates

[0040] (1) Preparation of methyltestosterone hapten

[0041] Take 0.5g of methyltestosterone and add 20mL of ethanol to dissolve, ...

Embodiment 2

[0075] The detection of methyltestosterone residue in the sample of embodiment 2

[0076] 1. Sample pretreatment

[0077] Take the fat-free animal tissue sample and homogenize it in a homogenizer for 1min (10000r / min), or cut it into pieces with scissors; weigh 2.0±0.05g of the homogenized tissue sample into a 10mL polystyrene centrifuge tube, add 1mL sample extract, shake well; then add 1mL sample diluent, shake well, absorb the supernatant for detection.

[0078] 2. Test with test strips

[0079] Use a dropper to drop 2 to 3 drops of the sample into the hole of the test paper, and observe the result after 5 to 10 minutes. If it exceeds 10 minutes, the test result of the sample will be invalid.

[0080] 3. Analyze test results

[0081] Negative: When (C) line shows red bands, (T) line shows red bands at the same time, and the color of (T) line is close to or darker than (C) line, it is judged as negative, expressed by "-", Such as figure 2 a, 2b shown.

[0082] Positiv...

Embodiment 3

[0084] Example 3 sample detection example

[0085] 1. Detection limit test

[0086] Take blank pork, chicken, fish, and shrimp samples, add methyltestosterone to them respectively to a final concentration of 0.5, 1.0, and 2.0 mg / kg, take test strips for detection, and repeat the measurement three times for each sample.

[0087] When testing pork, chicken, fish, and shrimp samples with test strips, when the concentration of methyltestosterone is 0.5mg / kg, the test strips show that the color of the T line is darker than that of the C line or that it is darker than the C line. The color development is consistent and negative; when the added concentration of methyltestosterone is 1.0 or 2.0mg / kg, the test strip shows that the T line color is lighter than the C line color or the T line does not develop color, which is positive, indicating that The detection limit of this test strip for methyltestosterone in pork, chicken, fish, shrimp and other tissue samples is 1.0mg / kg.

[0088...

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Abstract

The invention provides a colloidal gold test strip for detecting methyltestosterone residue. The colloidal gold test strip includes a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottom plate. The reaction film has a detection line coated with a methyltestosterone semiantigen-carrier protein conjugate and a quality control line coated with a goat-anti-mouse anti-antibody, and the conjugate release pad is sprayed with a methyltestosterone monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting methyltestosterone by using the colloidal gold test strip, which comprises the following steps: pre-treating a sample, then detecting the sample by the colloidal gold test strip, and finally analyzing the detection result. The colloidal gold test strip can be used for detecting the methyltestosterone residue in the animal tissue samples such as pork, chicken, fish and shrimp, has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, etc., and is suitable for screening of a large number of samples and field monitoring.

Description

technical field [0001] The invention relates to the technical field of rapid immunochemical detection of veterinary drug residues, and more specifically relates to a colloidal gold test strip for detecting methyltestosterone residues, which can quickly detect methyltestosterone residues by means of an immunochromatographic reaction using colloidal gold markers for color development. [0002] technical background [0003] Methyltestosterone (17α-Mehthyltestosterone, MT) is a synthetic steroid drug, also known as methyltestosterone, methyltestosterone and methyltestosterone, belonging to anabolic hormones. In aquaculture, as a sex hormone, it can not only promote the maturation of organs and the development of secondary characteristics, but also promote the assimilation of protein synthesis, increase muscle growth and weight gain, and is used as a sex hormone and Tonics are added to the feed. Due to the long metabolism of methyltestosterone, it is ingested by the human body th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/577G01N33/558
CPCG01N33/743G01N33/558G01N33/577
Inventor 徐宏鑫
Owner 北京安达兴业科技有限公司
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