57results about How to "Suitable for screening" patented technology

The invention relates to a genetic detection method, in particular to a quantitative detection method for unusual genetic mutation. The quantitative detection method for unusual genetic mutation comprises the following steps: firstly, corresponding primer sequences are designed and prepared according to the unusual genetic mutation required for quantitative detection, wherein the 3' end of a primer is provided with a mutant site - special nucleotide; except for the final site of the nucleotide, all the other sequences can be synthesized by commercial means; and the final site of the nucleotide is modified nucleotide, so as to make the primer incapable of extending in the general PCR reaction; and the modified nucleotide is added to the tail of the primer; and secondly, in a reaction system which contains special DNA polymerase and pyrophosphate, the primer designed and prepared in the first step is adopted for amplifying the unusual genetic mutation for quantitative detection, and a real-time fluorescent PCR instrument is utilized for quantitative detection; and the quantitative detection information of the unusual genetic mutation can be acquired through analysis of an amplifying curve recorded by the real-time fluorescent PCR instrument.

The invention provides an immune colloidal gold test paper card for testing quinolones, comprising a reaction film, a sample pad, a conjugate release pad, a water absorbing pad, and a back liner, wherein, the reaction film comprises a test zone coated with quinolones-carrier protein conjugate and a quality control zone coated with sheep-anti-mouse IgG; the conjugate release pad coats quinolones monoclonal antibody-colloidal gold marker. The invention also provides the method using the test paper card to test quinolones, which comprises the following steps: firstly, pre-treating sample; secondly, testing the sample with the test paper card; thirdly, analyzing test result. The invention can be used to test residual quantity of quinolones in animal derived food such as chicken, chicken liver, pork, pig liver, urine, and honey, and has the advantages of simple operation, high sensitivity, fast test, low cost, on-site supervision, and satisfaction of test requirement of a large quantity of samples.

The invention discloses a method for detecting maduramicin and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a maduramicin monoclonal antibody. The maduramicin monoclonal antibody is secreted by the hybridoma cell line MAD of the maduramicin monoclonal antibody, whose preservation number is CGMCC No. 3391. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the maduramicin in animal muscles and livers. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the maduramicin monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.

The present invention provides an enzyme-linked immunoassay kit for detecting dimethomorph. The enzyme-linked immunoassay kit contains an enzyme labeled plate coated with a dimethomorph-conjugated antigen, a dimethomorph standard substance solution, a concentrated enzyme conjugate, an enzyme conjugate working solution, a substrate chromogenic solution and a terminating solution. The invention further discloses a method for detecting dimethomorph in a sample by using the enzyme-linked immunoassay kit, wherein the method comprises: pre-treating a sample, detecting by using the kit, and analyzingthe detection result. According to the present invention, the enzyme-linked immunoassay kit can be used for detecting the residual dimethomorph in vegetable samples (raw materials, matching materialsand concentrated materials), has advantages of simple operation, low cost, high sensitivity and on-site monitoring, and is suitable for screening of a large number of samples.

The invention provides an enzyme linked immunosorbent assay (ELISA) kit for detecting vitamin B12. The enzyme linked immunosorbent assay kit comprises an ELISA plate coated with a vitamin B12 coupling antigen, an enzyme labeling ant-antibody working solution, a vitamin B12 specific antibody working solution, a vitamin B12 standard substance solution, substrate developing liquid, a stop solution, a concentration scrubbing solution and redissolution liquid. The invention also discloses a method for detecting the vitamin B12 by using the enzyme linked immunosorbent assay kit. The method comprises the following steps: firstly carrying out sample pretreatment, then detecting by using the kit, and finally analyzing detection results. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of vitamin B12 in cereals (maize meal, soybean meal, millet meal and rice meal), milk and milk powder, is simple and convenient to operate, low in cost and high in sensitivity, can realize on-site monitoring and is suitable for screening lots of samples.

The invention relates to a pesticide biological activity testing method that uses Cyclops as acting object. In slight amount vessel, the sensitivity of the sample would be used to take insecticidal activity test by Cyclops. The method is easy to operate and is suited to test biology of slight amount sample. The method could be used to test the activity of the existing variety and research the new pesticides.

The invention provides a mycobacterium tuberculosis early culture filtrate protein, its preparation method and an application of the protein in reagents and kits for diagnosing tuberculosis and latent infection people of mycobacterium trberculosis. The preparation method of the protein comprises the following steps: inoculating H37Rv strain to an inclined plane medium, inoculating the cultured strain to a liquid Sauton medium, conducting shaking culturing for 5 days at 37 DEG C, and then separating.

The invention provides a colloidal gold test strip for detecting methyltestosterone residue. The colloidal gold test strip includes a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottom plate. The reaction film has a detection line coated with a methyltestosterone semiantigen-carrier protein conjugate and a quality control line coated with a goat-anti-mouse anti-antibody, and the conjugate release pad is sprayed with a methyltestosterone monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting methyltestosterone by using the colloidal gold test strip, which comprises the following steps: pre-treating a sample, then detecting the sample by the colloidal gold test strip, and finally analyzing the detection result. The colloidal gold test strip can be used for detecting the methyltestosterone residue in the animal tissue samples such as pork, chicken, fish and shrimp, has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, etc., and is suitable for screening of a large number of samples and field monitoring.

The invention provides the rapid extraction procedures of the DSP in a shellfish sample aiming at the problems of complicated sample pretreatment, great workload, high cost and great time consumption in the existing liquid chromatography-mass spectrometry (LC-MS) technique for detecting a DSP (Digital Signal Processor). In the method provided by the invention, the UP LC / Q-TOFMS (Flight Mass Spectrometry) detection in the LC-MS technique are utilized and the UP LC / Q-TOFMS detection is used for the actual detection of merchant marine shellfish, thereby providing an effective and accurate detection technique for poisonous red tide research and food safety inspection.

The invention belongs to the field of drug analysis, relates to a method for screening an active component in a traditional Chinese medicine, and specifically, relates to a method for screening a traditional Chinese medicine active component with a function of hexokinase inhibition effects. Based on hexokinase catalytic reaction adopting adenosine triphosphate (ATP) as a substrate, the method can screen out a traditional Chinese medicine active component with a function of hexokinase inhibition effects through quantitative analysis processes on ATP and product ATP. The method is rapid, simple, economic and effective. Compared with a cell test method, the method reduces an extract use amount, saves biochemical reagents, and shortens a screening period. Therefore, the method has great importance for screening and research of traditional Chinese medicine active components. In the invention, all experiment parameters of an improved liquid chromatography-mass spectrometry (LC-MS) method have good reference values for mass spectrometry technology-based determination of other biomolecules with similar properties.

A detection method includes using PAT protein as antigen to immunize animal for obtaining specific poly antibody, cross - linking the antibody on agarose gel to prepare immuiaffinity chromatographic column, using affinity column to enrich PAT protein after detected sample is processed, collecting affinity column eluent and carrying out enzyme - linked immunosorbant assay for confirming PAT protein content in it. The kit of the method is also disclosed.

Disclosed is a method for producing an antibody, which comprises the steps of: immunizing a tissue containing a cell to be immunized in vitro in a liquid culture medium containing an antigen and a stimulant; selecting an immunized cell; and producing the antibody from the selected immunized cell. The method enables to produce an antibody in large quantity within a short period, and therefore can contribute to the widespread use of an immunological test.

The invention discloses an olaquindox metabolin antigen, an antibody, an enzyme-linked immunosorbent assay kit and a detection method. 4-(3-methyl-2-naphthalene carboxamide) butyrate is taken as an olaquindox metabolin hapten; the olaquindox metabolin antigen can be acquired after the hapten is coupled with carrier protein; the olaquindox metabolin antigen can be used for preparing an olaquindox metabolin specific antibody; the specific antibody can be used for preparing the enzyme-linked immunosorbent assay kit or colloidal gold test paper card used for detecting the residue of olaquindox metabolin in food. The invention also discloses the corresponding detection method of the kit. The method is simple, convenient and quick and is characterized by linear detection range within 0.2-16.2ng / mL, sensitivity of 0.54ng / mL, limit of detection of 0.32ng / mL, recovery rate within 80%-109.8%, low detection limit, high sensitivity, high specificity, high stability, low cost and suitability for mass sample detection and onsite quick detection.

The invention provides an ELISA kit for detecting roxarsone. The ELISA kit for detecting roxarsone comprises an ELISA plate coated with a roxarsone coupled antigen, a roxarsone standard substance solution, a concentrated enzyme conjugate, a enzyme conjugate operating fluid, a substrate colorimetric solution and a stopping solution. The invention also discloses a method using the ELISA kit to detect roxarsone in a sample. The method comprises the following steps: preprocessing the sample, detecting roxarsone by using the kit, and analyzing a detection result. The ELISA kit can be used to detect the residual quality of roxarsone in the feed (raw material, batch and concentrate) sample, and has the advantages of simple operation, low cost, high sensitivity, realization of onsite monitoring, and suitableness for screening a large amount of samples.

The invention discloses a method for detecting melamine and an enzyme-linked immunosorbent assay (ELISA) kit special for same. The enzyme-linked immunosorbent assay kit comprises melamine monoclonal antibody and melamine hapten. The melamine monoclonal antibody is secreted by a melamine monoclonal antibody hybridoma cell strain MAL with the collection number of CGMCC No.3394. The enzyme-linked immunosorbent assay kit mainly performs qualitative or quantitative detection on the content of melamine in edible products for animals and human (particularly milk products such as milk and milk powder, eggs, feeds and the like) by an indirect competitive ELISA method. The kit and the detection method have low requirement on pretreatment of a sample; the pretreatment process of the sample is simple; and massive samples can be simultaneously detected quickly. The melamine monoclonal antibody with high specificity is adopted, and the detection method is convenient and practicable and has the characteristics of high specificity, high sensitivity, high accuracy and the like.

The invention discloses a production process of silicon carbide micropowder, comprising the following steps: S1 sorting silicon carbide, S2 diluting semi-solid silicon carbide powder, S3 atomizing silicon carbide powder liquid, and S4 silicon carbide powder Drying, S5 to make finished silicon carbide powder, the vibration frequency of the ultrasonic vibrating screen is 50HZ, and the collector is a glass container. The silicon carbide is sorted by a hydraulic grading conical cylinder, which has a fast screening speed and can quickly separate the ultrafine silicon carbide. The atomization duration of the silicon carbide micropowder production process is 5 seconds, and the drying The time is 10 seconds. Since the silicon carbide is atomized, the contact area between the silicon carbide and the hot air increases to enhance the drying efficiency of the silicon carbide. The invention is suitable for batch screening and drying of silicon carbide, has good practicability and is suitable for further popularization.

The invention belongs to the technical field of oil and gas geochemistry, and relates to a method for evaluating hydrocarbon generation potential of hydrocarbon source rock. The method comprises the following steps that S1, selecting hydrocarbon source rock, and obtaining the total organic carbon amount of the hydrocarbon source rock; S2, separating a plurality of organic microcomponents in the hydrocarbon source rock to obtain each organic microcomponent in the hydrocarbon source rock; S3, performing a rock pyrolysis experiment on each organic microcomponent to obtain the hydrocarbon generation potential of each organic microcomponent; S4, calculating the proportion of each organic microcomponent in the plurality of organic microcomponents; and S5, calculating the product sum of the hydrocarbon generation potential of each organic microcomponent and the proportion of each organic microcomponent in the plurality of organic microcomponents, and the product of the product sum of the hydrocarbon generation potential of each organic microcomponent and the total organic carbon amount of the hydrocarbon source rock, so as to obtain the hydrocarbon generation potential of the hydrocarbonsource rock. The method is simple and rapid to operate and suitable for screening a large number of samples.

An expression of an ergosterol biosynthetic enzyme such as ERG6 and ERG3 is regulated with an inducible promoter, and transformation is performed in a state where the enzyme is expressed, and then screening or assay of drug-resistant colonies is performed in a state where the expression of the enzyme is repressed.

The invention discloses a hapten for isoprothiolane content detection and a preparation method and application thereof, particularly relates to a hapten for isoprothiolane enzyme-linked immunosorbent assay, and further discloses a preparation method of the hapten and application of the hapten in antibodies and kits. The enzyme linked immunosorbent assay kit established on the basis of the isoprothiolane hapten can rapidly detect products, and is convenient to use and low in detection cost, and the detection method is efficient, accurate and rapid, can simultaneously detect a large batch of samples, and is suitable for on-site monitoring of isoprothiolane in agricultural products, especially tobacco and screening of a large number of samples.

The invention discloses a kit and multiple PCR detecting method for synchronously detecting the wide compatibility gene S5 and the erect panicle gene DEP1 of paddy rice and belongs to the technical field of agricultural biology. The kit and multiple PCR detecting method has the advantages that the functional markers of the wide compatibility gene S5 and the erect panicle gene DEP1 are designed according to the difference between the wide compatibility gene S5 and non-wide-compatibility genes and the difference between the erect panicle gene DEP1 and non-erect-panicle genes, a PCR reaction system and procedure are optimized to build the multiple PCR method capable of synchronously detecting the wide compatibility gene S5 and the erect panicle gene DEP1, the two target genes can be detected by one PCR reaction, the multiple PCR method is simple, efficient, sensitive and economical as compared with a single PCR method, and the multiple PCR method is suitable for screening, authenticating and molecular pyramiding breeding of wide compatibility and erect panicle seed resources and significant to the increasing of pyramiding breeding molecule selection efficiency and cost lowering.

The invention discloses a reagent kit for detecting o-allyl phenol and a special antibody thereof. The reagent kit comprises a monoclonal antibody of the o-allyl phenol or a polyclonal antibody of the o-allyl phenol, wherein the monoclonal antibody of the o-allyl phenol is generated by the excretion of mouse hybridoma cell line mAb225# with the preserving number of CGMCC No.2905. The enzyme linked immune reagent kit for detecting o-allyl phenol and a method for detecting the o-allyl phenol can be used for detecting the residual quantity of the o-allyl phenol in farm products such as plant tissues, has the advantages of simple sample preliminary treatment course, simple and convenient operation, low cost, good specificity, high sensitivity and precision, and the like, can monitor on site and is suitable for screening a plurality of samples, therefore, the method for detecting the o-allyl phenol and the special reagent kit thereof can exert an important function in detecting the residual o-allyl phenol chemical in the farm products.

The invention provides an enzyme linked immunosorbent assay kit for detecting omethoate. The kit comprises an enzyme label plate coated with an omethoate coupled antigen, an omethoate standard substance solution, a concentrated enzyme conjugate, an enzyme conjugate operating fluid, a substrate coloring solution and a stopping solution. The invention also discloses a method using the enzyme linked immunosorbent assay kit to detect omethoate in a sample. The method comprises the following steps: preprocessing the sample, detecting omethoate by using the kit, and analyzing a detection result. The enzyme linked immunosorbent assay kit can be used for detecting the residual quantity of omethoate in vegetable (raw material, batch and concentrate) samples, and has the advantages of simplicity in operation, low cost, high sensitivity, realization of onsite monitoring, and suitableness for screening a lot of samples.

The invention provides a rapid detection method of benzodiazepine and an operation vessel combination thereof. The rapid detection method comprises the following steps of performing trituration on materials to be detected, adding an acid solution for extraction and obtaining an upper layer of extracting solution; adding a chromogenic agent into the extracting solution to perform a chromogenic reaction, determining whether a compound of benzodiazepines is added into the materials to be detected or not according to a chromogenic result and determining that the compound of benzodiazepines is contained in the materials to be detected if orange-red precipitates are generated in the extracting solution; determining that the compound of benzodiazepines is not contained in the materials to be detected if the orange-red precipitates are not generated in the extracting solution. According to the rapid detection method of the benzodiazepine and the operation vessel combination thereof, samples are dissolved through the dissolved liquid, the chromogenic effect of the samples is achieved through the chromogenic agent, the chromogenic result can be observed through naked eyes, the corresponding operation vessel combination is simple in operation and convenient to carry, expensive instrument equipment are not required, the detection process is rapid, the accuracy is high, and the corresponding operation vessel combination is suitable for screening of the large quantities of samples.

The invention provides an in-vitro metabolic activity detection system based on intestinal microorganisms. The in-vitro metabolic activity detection system comprises the following steps of acquiring excrement samples, and screening the excrement samples through an excrement detector; according to a screening result, reserving excrement samples without organic lesions; carrying out activity detection on the excrement samples without the organic lesions; detecting the metabolic value of live intestinal bacteria according to an activity detection result; comparing the metabolic value of the intestinal bacteria with pre-stored information, and performing gene sequencing on the intestinal bacteria with large difference in the comparison value; determining structural characteristics of intestinal flora according to a gene sequencing result; and obtaining a biomarker at the early stage of diabetes according to the structural characteristics of intestinal flora. An intestinal microorganism in-vitro metabolic activity detection system is used for carrying out in-vitro fermentation on excrement of different subtypes and normal people, metabolic response values corresponding to different prebiotics are detected, including gas production, acid production, degradation degree of the prebiotics and promotion effect on probiotics, and therefore, the overall metabolic level of intestinal microorganisms is comprehensively reflected.

The invention discloses a fast detection method of aminohydantoin hydrochloride. The fast detection method comprises the following steps: (a) manufacturing a fluorescence sensitization standard curve: preparing a series of aminohydantoin hydrochloride standard solutions with different concentrations, and respectively transferring each aminohydantoin hydrochloride standard solution to a colorimetric tube of 5mL, adding 1.0mL of CdTe solution with concentration of 7.25*10<-4> mol / L in each colorimetric tube, metering volume by use of a phosphate buffer solution with pH of 7.4, uniformly shaking, placing at room temperature for 5-10min, and detecting the fluorescence intensity F of each system by use of a molecular fluorophotometer; and meanwhile, adding 1.0mL of CdTe solution with concentration of 7.25*10<-4> into the colorimetric tube of 5mL. The fast detection disclosed by the invention has the characteristics of being high in sensitivity, simple in sample preprocessing, transient in detection time, and large in detection sample amount, and suitable for on-site supervision and the screening of mass samples.

The invention discloses a clenbuterol, ractopamine and salbutamol disposable method for euzyme linked immunosorbent assay detection, which is characterized in that a monoclonal antibody is precoated on an enzyme label plate, clenbuterol, ractopamine and salbutamol in a sample to be detected and enzyme-labelled antigen compete for binding with the coated monoclonal antibody, a developer is used for developing, the content of clenbuterol, ractopamine and salbutamol in the sample to be detected is in inverse proportion with percent absorbency of the sample to be detected, compared with a standard curve, the content of clenbuterol, ractopamine and salbutamol in the sample to be detected can be obtained, and a special detection kit used for the above mentioned detection method comprises the enzyme label plate, a standard substance solution, a concentrated enzyme marker, an enzyme marker diluents, the developer, a concentrated washing lotion and a stopping solution. The invention uses a direct competition enzyme linked immunosorbent assay technology, three kinds of clenbuterol hydrochloride which are clenobuterol hydrochloride, ractopamine and salbutamol are performed with disposable analysis detection, the usage is simple, the specificity is high, and the detection scope is 0.1ng / mL-2mumg / mL.

The invention relates to a DNA microarray chip, in particular to an integrated high-flux detection DNA microarray chip for hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus 1 (HIV-1). A solid-phase carrier substrate is used, and an oligonucleotide probe is designed in view of genes of HBV, HCV and HIV-1 so as to prepare the DNA microarray chip; and when matched witha PCR primer and other components, the DNA microarray chip can simultaneously detect the three viruses in a plurality of samples with high sensitivity and high specificity. The DNA microarray chip ismainly used for screening the virus nucleic acid in blood and blood products.

The invention discloses a kit and a multiplex PCR detection method for synchronously detecting rice broad affinity gene S5 and erect panicle gene DEP1, belonging to the field of agricultural biotechnology. According to the difference between broad affinity gene S5 and non-broad affinity, and the difference between erect ear gene DEP1 and non erect ear, the present invention designs the functional markers of the two genes. The multiplex PCR method of the gene S5 and the erect panicle gene DEP1 can detect two target genes in one PCR reaction. Compared with the single PCR method, the operation is more simple, efficient, sensitive and economical. This method is suitable for broad compatibility and erect panicle germplasm. The screening, identification and molecular aggregation breeding of resources are of great significance to improve the molecular selection efficiency of aggregation breeding and reduce the cost.

The invention discloses a hapten for detecting the content of rice blast spirit, a preparation method and application thereof, and in particular relates to a hapten for enzyme-linked immunoassay detection of rice blast spirit. The invention also discloses a preparation method of the hapten and its application. Application in antibodies and kits. The rapid detection product of the enzyme-linked immunosorbent assay kit based on the rice blast hapten is easy to use, low in detection cost, efficient, accurate, fast, and can detect a large number of samples at the same time. It is suitable for agricultural products, especially the rice blast in tobacco. On-site monitoring and screening of large samples.

The invention provides an antibody aiming at yellow fever virus NS1 protein and application thereof. The antibody comprises a YD40 antibody and / or a YB36 antibody; the invention provides a highly sensitive yellow fever virus ELISA double-antibody sandwich detection method. The method can be operated in a common laboratory, can be further developed into various kits, such as colloidal gold, ELISA kits and the like, and is convenient to use.