68results about How to "Good quantitative effect" patented technology

An analytical method for quantitative gene expression amount determination and comparison has great significance for disease-related gene screening and in clinical early diagnosis and development of specific medicine. The present invention utilizes quantitative characteristic of biological luminance process is sequencing to compare the gene expression amount of the same gene in different individuals or samples and to search disease-related gene. The specific steps includes: making mRNA with different sources by using a proper method and mixing in equal amount to form PCR substrate; PCR amplification with the primer corresponding to the gene of different sources and gene specific primer; and determining sequence by using bioluminescence analysis process with base variety to replace gene of different source.

An object of the present invention is to provide a method for producing a stable isotope-labeled target peptide fragment in mass spectrometry, which achieves inexpensive and convenient production. As a solution to attain the object, the stable isotope-labeled target peptide fragment in mass spectrometry is produced using a method comprising the steps of: expressing a DNA conjugate in a system having a stable isotope-labeled amino acid to thereby prepare a stable isotope-labeled protein, wherein the DNA conjugate comprises: a tandemly linked DNA in which two or more DNAs encoding one or more types of target peptide fragments are linked in tandem; and a DNA encoding a peptide fragment for concentration measurement; subjecting the stable isotope-labeled protein to fragmentation treatment with trypsin to prepare a stable isotope-labeled peptide fragment for concentration measurement and stable isotope-labeled target peptide fragments; quantifying the stable isotope-labeled peptide fragment for concentration measurement using a liquid chromatograph-tandem mass spectrometer (LC / MS / MS); and calculating the concentration of the stable isotope-labeled target peptide fragment of each type from the quantification value of the stable isotope-labeled peptide fragment for concentration measurement.

The invention relates to a method for predicting a low-porosity and low-permeability clasolite effective reservoir at a high-diagenesis stage and belongs to the technical field of oil and gas exploration and development. The method is characterized in that a geological process response relationship is established through carrying out analysis, simulation and quantitative evaluation on a geological process affecting porosity, the original pore preservation and induced pore increase / decrease conditions of a reservoir are determined, thus, the current porosity of a low-porosity and low-permeability reservoir at the high-diagenesis stage is quantitatively evaluated, the development of the effective reservoir is predicted, and a basis is provided for reservoir evaluation. The method for predicting the low-porosity and low-permeability clasolite effective reservoir at the high-diagenesis stage has the advantages that the predictability is strong, the distribution of various clasolite effective reservoirs at different stages of high-diagenesis can be accurately predicted, the fine prediction on the distribution of effective reservoirs of a research target section can be achieved, and the predicted unit plane can reach a hectometer level; the theoretical basis is solid, and the condition of over reliance on the data of sampled points in the traditional method is avoided; the quantitative performance is good, the applicability is wide, and the information requirements are relatively low; and the evaluation, with different information foundations, on different exploration and development stages is facilitated.

The present invention relates to an analytical method for quantitatively comparing expression amount of identical gene in different origins, wherein the gene expression amount difference of the identical gene in different source tissues or cells are quantitatively compared, by adopting the quantitatively specific characters of bioluminescence method and the principle that different deoxyribonucleoside triphosphates (dNTP) are added therein one by one. The concrete steps are as follows: the messenger RNAs (mRNA) from different sources are inverse transcribing into cDNA, and a section source specificity DNA sequence for cDNA from each source is signed; the signed cDNA from different sources are mixed in a tube, as a substrate of a polyase chain reaction (PCR); a common primer and a gene specificity primer are used to execute PCR enlargement; bioluminescence analytical method is adopted to determine the base sequences, wherein the base species represent different sources of genes, and the signal intensity represents the expression amount of genes form each source. Said method exhibits great significance for disease related gene selecting, clinical early diagnosis and creation of specific medicines for treating diseases.