68results about How to "Good quantitative effect" patented technology

The invention relates to a method for predicting a low-porosity and low-permeability clasolite effective reservoir at a high-diagenesis stage and belongs to the technical field of oil and gas exploration and development. The method is characterized in that a geological process response relationship is established through carrying out analysis, simulation and quantitative evaluation on a geological process affecting porosity, the original pore preservation and induced pore increase / decrease conditions of a reservoir are determined, thus, the current porosity of a low-porosity and low-permeability reservoir at the high-diagenesis stage is quantitatively evaluated, the development of the effective reservoir is predicted, and a basis is provided for reservoir evaluation. The method for predicting the low-porosity and low-permeability clasolite effective reservoir at the high-diagenesis stage has the advantages that the predictability is strong, the distribution of various clasolite effective reservoirs at different stages of high-diagenesis can be accurately predicted, the fine prediction on the distribution of effective reservoirs of a research target section can be achieved, and the predicted unit plane can reach a hectometer level; the theoretical basis is solid, and the condition of over reliance on the data of sampled points in the traditional method is avoided; the quantitative performance is good, the applicability is wide, and the information requirements are relatively low; and the evaluation, with different information foundations, on different exploration and development stages is facilitated.

The present invention relates to an analytical method for quantitatively comparing expression amount of identical gene in different origins, wherein the gene expression amount difference of the identical gene in different source tissues or cells are quantitatively compared, by adopting the quantitatively specific characters of bioluminescence method and the principle that different deoxyribonucleoside triphosphates (dNTP) are added therein one by one. The concrete steps are as follows: the messenger RNAs (mRNA) from different sources are inverse transcribing into cDNA, and a section source specificity DNA sequence for cDNA from each source is signed; the signed cDNA from different sources are mixed in a tube, as a substrate of a polyase chain reaction (PCR); a common primer and a gene specificity primer are used to execute PCR enlargement; bioluminescence analytical method is adopted to determine the base sequences, wherein the base species represent different sources of genes, and the signal intensity represents the expression amount of genes form each source. Said method exhibits great significance for disease related gene selecting, clinical early diagnosis and creation of specific medicines for treating diseases.