Fast detection method of aminohydantoin hydrochloride

Abstract

The invention discloses a fast detection method of aminohydantoin hydrochloride. The fast detection method comprises the following steps: (a) manufacturing a fluorescence sensitization standard curve: preparing a series of aminohydantoin hydrochloride standard solutions with different concentrations, and respectively transferring each aminohydantoin hydrochloride standard solution to a colorimetric tube of 5mL, adding 1.0mL of CdTe solution with concentration of 7.25*10<-4> mol / L in each colorimetric tube, metering volume by use of a phosphate buffer solution with pH of 7.4, uniformly shaking, placing at room temperature for 5-10min, and detecting the fluorescence intensity F of each system by use of a molecular fluorophotometer; and meanwhile, adding 1.0mL of CdTe solution with concentration of 7.25*10<-4> into the colorimetric tube of 5mL. The fast detection disclosed by the invention has the characteristics of being high in sensitivity, simple in sample preprocessing, transient in detection time, and large in detection sample amount, and suitable for on-site supervision and the screening of mass samples.

Description

technical field [0001] The invention relates to a detection method, in particular to a rapid detection method of nitrofurazone metabolites. Background technique [0002] Nitrofurans are often used as broad-spectrum antibiotics to prevent and treat gastrointestinal diseases in pigs, cattle, poultry and bees caused by Salmonella and Escherichia. However, in the course of long-term experimental research, it was found that both nitrofuran drugs and metabolites can cause cancer and gene mutations in experimental animals, and such drugs are prohibited from being used in treatment and feed. Since the prototype drug of nitrofuran is rapidly metabolized in the body, it cannot be detected, but its metabolites are quite stable due to the combination with proteins, so when analyzing the residues of such drugs, it is often necessary to analyze the metabolized products, and the management department uses The detection of metabolites is a means to achieve the purpose of detection of nitro...

AI Technical Summary

Problems solved by technology

At present, the most common methods used to detect nitrofuran metabolites are high performance liquid chromatography (HPLC), liquid chromatography-mass chromatography (LC-MS / MS), etc. These methods have high sensitivity, accurate results, good repeatability, and false positives. There are few positives, but the sample pretreatment process is complicated, the degree of instrumentation is high, and the price is expensive