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Quick production method of resting egg of fresh water rotifer

A production method and technology of dormant eggs, applied in animal husbandry and other fields, can solve problems such as inability to guarantee bait supply

Inactive Publication Date: 2012-05-02
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Feed supply cannot be guaranteed during fish fry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Rotifer monoclonal selection

[0016] (1) Category screening

[0017] Use No. 13 floating net to fish out 200 mL of live samples from the surface of the culture pond. Take it back to the laboratory and add filtered pond water to dilute to 5L. Add 95% trichlorfon emulsion at a concentration of 10 ppm, filter after 10 minutes, pick one carrying non-mixed female, and transfer it to a microwell cell culture plate. Add Nannochloropsis concentration 3×10 to the microwell 6 ind / mL EPA mixed suspension. Continuous light cultivation under 20-40W fluorescent lamp, temperature 25-28°C. After 2 days, they were examined under a stereomicroscope, isolated, expanded and cultured.

[0018] (2) Expansion of training

[0019] Use a 16×150 mm test tube, add 10 mL of a suspension prepared from rotifer culture medium EPA and centrifuged microalgae, and culture under continuous light at 20-40W fluorescent lamp at a temperature of 25-28°C. After expanding the culture...

Embodiment 2

[0020] Embodiment 2: the production example of rotifer dormant eggs

[0021] The concentration of methyltestosterone is 10, 15, 30, 50 and 1000 μg / L, the density of inoculated rotifers is 0.5ind / mL, centrifuge Nannochloropsis at 2500-5000 rpm for 10 minutes or use flocculation to settle, adjust the algae concentration 3× with EPA 10 6 ind / mL, cultivate 2 20-40W fluorescent tubes under continuous light. The temperature is 25-28°C, no food is added during production, and harvested after 5 days. The yield of resting eggs is (see Table 1): the average yield without hormone addition is 166 ind / 10mL, the average yield with 10μg / L alpha testosterone is 178.75ind / 10mL, and the average yield with 15μg / L is 186.75 ind / 10mL , the average yield was 202.25 ind / 10mL when 30μg / L was added. Addition of 50 and 1000 μg / L was toxic and inhibitory (mean yields were 40.25 ind / 10mL and 13.75 ind / 10mL, respectively). Statistical analysis showed that adding 30μg / L could significantly increase t...

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PUM

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Abstract

The invention relates to a quick production method of resting egg of fresh water rotifer, comprising the steps of inoculating propagated rotifer to Nannochloris oculata; adding 10-30 mu g / L of Methyltestosterone, wherein the inoculating rotifer density is 0.5 ind / mL; centrifugating Nannochloris oculata for 10 min at 2500-5000 r / min, condensing and collecting or collecting Nannochloris oculata by a flocculent settling method; using a rotifer culture liquid to regulate Nannochloris oculata density to be 3*106 ind / mL; using two 20-40 W of fluorescent tubes to carry out illuminating continuously, wherein the temperature is 25-28 degrees centigrade and no other food is added during the production; harvesting resting egg after 5 days. The method is simple, convenient, quick and economic, is convenient to promote and apply and can obtain high-density rotifer resting egg at short time.

Description

technical field [0001] The invention belongs to the fields of biotechnology and ecology environment inspection (monitoring) of the subject of aquatic products, and in particular relates to a rapid production method of resting eggs of freshwater rotifers. Background technique [0002] Rotifer (rotifer) is the main functional group in the freshwater ecosystem, and more than 90% of the fish eat early biological bait, and they are also the test animals for environmental pollution and environmental risk assessment. The resting eggs of rotifers are the source of provenance for large-scale production of bait and the standard starting material for ecotoxicology research. In the aquaculture industry, rotifers play a pivotal role in the survival rate and growth rate of seedlings. At present, the aquatic production sector basically relies on a large amount of labor to fish in natural water bodies during the fish breeding period. Feed supply cannot be guaranteed during fish fry. With...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 杨家新
Owner NANJING NORMAL UNIVERSITY
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