Preparation method and application of releasing agent

By designing a universal release agent containing components such as amide compounds, metal chelates, etc., the problem of difficult release of steroid hormones in the serum is solved, and more accurate and efficient hormone detection is achieved, which simplifies the experimental process and improves efficiency.

CN119716033APending Publication Date: 2025-03-28BEIJING JISHUITAN HOSPITAL GUIZHOU HOSPITAL
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Patent Information

Application Number
CN202411904031.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-23
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The prior art is difficult to effectively release steroid hormones (such as testosterone, estradiol, progesterone, cortisol) in the serum from binding proteins, resulting in the impact of the accuracy and safety of clinical hormone project testing.

Method used

Provided is a universal release agent containing amide compounds (such as sodium α-mercaptoethylsulfonate and N-methylacetamide), metal chelates, preservatives, surfactants and steroid heterocyclic compounds. Through carefully designed compositions, the conformation of hormone binding proteins is changed and the release of hormone molecules is promoted.

Benefits of technology

This release agent can effectively release steroid hormones in the serum from binding proteins, improve the accuracy of detection, simplify the experimental process, reduce the workload of experimental personnel, and improve the experimental efficiency.

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Abstract

The invention relates to a preparation method and application of a releasing agent, and belongs to the technical field of steroid hormone releasing agents. The releasing agent is prepared from the following components in percentage by mass: 0.2 to 1 percent of an amide compound, 0.02 to 0.06 percent of a metal chelate, 0.05 to 0.15 percent of a preservative, 0.2 to 0.5 percent of a surfactant, 0.015 to 0.045 percent of a steroid heterocyclic compound and the balance of deionized water, wherein the amide compound is a composition of sodium alpha-mercaptoethanesulfonate and N-methylacetamide, and the amide compound is a compound of sodium alpha-mercaptoethanesulfonate and N-methylacetamide; the steroid heterocyclic compound is a composition of methyltestosterone, danazol and stanolone. According to the universal releasing agent, the four hormones in a sample can be effectively released from respective binding proteins to be in a free state, and clinical test data of the sample can be obtained by measuring the content of the free hormones, so that the measured test result is more accurate.
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