Novel application of mulgedium tataricum

A technology of lacto, applied in the direction of medical preparations containing active ingredients, plant raw materials, respiratory diseases, etc.

Inactive Publication Date: 2013-09-25
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The applicant has not seen any reports at home and abroad on the research on the effect of milk extract on cancer cells

Method used

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  • Novel application of mulgedium tataricum
  • Novel application of mulgedium tataricum
  • Novel application of mulgedium tataricum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment one: the processing of chicory

[0022] The milk lettuce ( Mulgedium tataricum (Linn.) DC.) Whole grass, shade-dried, stored at -80°C. Grind 8g of dried milk lettuce, soak it in 100ml boiling water, ultrasonicate for 1h, bathe in boiling water for 30min, filter twice, pack the raw solution into freeze-dried powder after 48h freeze-drying, re-dissolve the freeze-dried powder with culture medium, separate Cells were treated with final concentrations of 2 mg / ml, 1.5 mg / ml, 1.2 mg / ml, 0.8 mg / ml, 0.4 mg / ml.

Embodiment 2

[0023] Example 2: Different concentrations of milk extracts act on H1299 and A549 cells, and the effect on cell growth

[0024] Spread H1299 and A549 cells evenly in 24-well plates, grow for 24 hours, and when the cells adhere to the wall and grow well, use 2mg / ml, 1.5mg / ml, 1.2mg / ml, 0.8mg / ml, 0.4mg / ml milk lettuce extract treated cells for 48 hours, it was found that the control group grew well and the cells had good refractive properties, while in the treatment group, as the concentration of milk lettuce increased, the state of the cells became worse and worse, especially at 2mg / ml and 1.5mg / ml treatment group compared with the untreated group, the cells shrunk, the ability to adhere to the wall was poor, and the cell growth was slow.

Embodiment 3

[0025] Example 3: Lactocory water extract has inhibitory effect on the proliferation of lung cancer cells H1299 and A549

[0026] Spread H1299 and A549 evenly in 24-well plates, grow for 24 hours, and when the cells adhere to the wall and grow well, use final concentrations of 2mg / ml, 1.5mg / ml, 1.2mg / ml, 0.8mg / ml, When the cells were treated with 0.4mg / ml milk lettuce extract for 24h and 48h, the viability of the cells was detected with the CCK8 kit (Shanghai Qihai Futai Biological Co., Ltd.), and the detection wavelength was 450nm. The results showed that compared with the untreated group, the viability of the cells in the experimental group became worse and worse with the increase of the drug concentration, especially in the treatment groups of 2mg / ml, 1.5mg / ml, and 1.2mg / ml, the drug significantly inhibited cell proliferation and activity.

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Abstract

The invention discloses a novel application of mulgedium tataricum. A water extraction product of the asteraceae mulgedium plant mulgedium tataricum which can both be adopted as a medicine and as a food can strongly inhibit the growth of lung cancer cell H1299 and A549. With the plant, cancer cell mitochondrial membrane potential is reduced, intracellular reactive oxygen level is increased, and a series of physiological responses such as apoptosis is further caused. The discovery of the anticancer effect of the edible herb provides good basis for clinical medicines and the developments of novel medicines or health-care products.

Description

technical field [0001] The present invention relates to a new application of milk lettuce. Background technique [0002] According to the statistics of the World Health Organization (WHO), there are 10 million new cancer patients in the world every year, and 6 million people die of cancer. Cancer is almost synonymous with death. At present, the measures to treat cancer include surgery, chemotherapy, radiotherapy and immunotherapy, etc. 4 kinds, but its therapeutic effect is all unsatisfactory. The anti-tumor effect of traditional Chinese medicine has the characteristics of multiple targets, multiple links, and multiple effects. It can act on multiple links of tumor occurrence and development. In recent years, many reports have proved the advantages of traditional Chinese medicine in anti-tumor recurrence and metastasis. More and more patients with advanced cancer choose traditional Chinese medicine for treatment. Chinese medicine treatment has also entered a relatively syst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P11/00A61P35/00
Inventor 贺燕云华子义张彩艳刘小敏韦嘉励李艳利马中良
Owner SHANGHAI UNIV
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