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Pistil treatment reagent for promoting plant wide hybrid seed set and application method thereof

A technology of distant hybridization and reagents, applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, to achieve the effect of overcoming incompatibility, overcoming hybridization obstacles, and improving seed setting rate

Inactive Publication Date: 2016-07-13
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Aiming at the many problems existing in the existing distant hybridization, the present invention is based on solving the obstacles before fertilization, to improve the seed setting rate of distant hybridization, and provides a method mainly composed of boric acid, CaCl 2 , bovine serum albumin, mannitol solution, etc., spray the reagent on the pistil stigma 10-30 minutes before pollination, which is conducive to improving the seed setting rate and has a favorable impact on the survival rate of seedlings

Method used

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  • Pistil treatment reagent for promoting plant wide hybrid seed set and application method thereof
  • Pistil treatment reagent for promoting plant wide hybrid seed set and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, wheat distant hybridization test

[0029] experiment material:

[0030] Fifteen common wheat lines with excellent agronomic traits were used as female parents, including Shannong 18, Shannong 22, Shannong 23, Jimai 22, Liangxing 66, Liangxing 99, Tainong 18, Linmai 4, Shannong Nong 20, Luyuan 502, Jinan 17, Wennong 14, Yannong 21, Qingmai 6; the identified disease-resistant Elymus plant was used as the male parent for distant hybridization. Each hybrid combination was replicated 3 times, with at least 3 plants and 6 panicles in each replicate, and no reagent treatment was used as the control.

[0031] Reagents used: The effects of mannitol concentration, enzyme type and various enzyme concentrations in the enzyme solution were screened. The basic components of the enzyme solution are 0.02-0.04% boric acid, 0.4% CaCl 2 , 1.0% bovine serum albumin (BSA), 12g / 100ml mannitol, additional 0.04%-0.18% pectinase (Serva), 0.10%-0.15% cellulase (OnozukaRS), 0.10%...

Embodiment 2

[0046] Embodiment 2, the distant hybridization test of pyrethrum

[0047] experiment material

[0048] The experiment was conducted from September 2012 to December 2013 in the nursery of Yihe Garden Co., Ltd., Jinan City, Shandong Province. The female parent of the test is a conventionally cultivated pyrethrum variety, and the test material of the male parent is Taihang chrysanthemum, chamomile, heterochromia, Shennongxiangju, tetraploid wild chrysanthemum, autotetraploid chrysanthemum, hexaploid Maohua chrysanthemum, six Ploid feverfew and common cultivated chrysanthemum.

[0049] All materials come from the "China Chrysanthemum Germplasm Resource Preservation Center" of Nanjing Agricultural University.

[0050] Reagents used: The effects of mannitol concentration, enzyme type and various enzyme concentrations in the enzyme solution were screened. The basic components of the enzyme liquid are 0.04-0.06% boric acid, 0.6% CaCl2, 1.0% bovine serum albumin (BSA), 12.5g / 100ml m...

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Abstract

The invention relates to the field of plant breeding and provides a pistil treatment reagent for promoting plant wide hybrid seed set and an application method thereof. The reagent is mainly composed of boric acid, CaCl2, bovine serum albumin, mannitol solution and the like. The reagent is sprayed to the pistil stigma within 10-30 minutes before pollination, contributes to improving the setting percentage and has favorable influences on the seedling survival rate. By utilizing the method provided by the invention, cross-incompatibility among plant species can be effectively overcome, and the crossing barrier between ordinary wheat and pyrethrum and plants which is in the same family, but is in different genera, is effectively overcome. The experiment proves that the setting percentage of hybrid seeds is improved due to the method, the seed plumpness is high, and a novel way is provided for obtaining superior hybrid for plant wide hybrid.

Description

technical field [0001] The invention relates to the field of plant breeding, and provides a pistil treatment agent capable of promoting distant hybridization and fruiting of plants and an application method thereof. Background technique [0002] Distant hybridization refers to the hybridization between biological types of taxa above species, including interspecific hybridization of plants of the same genus, intergeneric hybridization of plants of different genus and species of higher taxa. Hybridization, such as inter-subtribal hybridization and intertribal hybridization, is an important way to realize distant gene transfer. [0003] Plant genetics and breeding technology, also known as variety improvement, is a technology for improving the genetic characteristics of crops and garden plants to cultivate high-yielding and high-quality varieties. It is a biotechnology with low investment and high benefits, and it is of great significance to the development of planting producti...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 张卫东
Owner SHANDONG AGRICULTURAL UNIVERSITY
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