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Method for artificially cultivating Ganoderma guinanense fruiting bodies

A technology for artificial cultivation of Ganoderma lucidum seeds in southern Guangxi, applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as unsuccessful artificial cultivation methods and limited wild resources

Active Publication Date: 2012-01-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Locals or folks believe that Ganoderma lucidum in South Guangxi has the effects of calming and analgesic, protecting liver and detoxifying, lowering blood fat and blood sugar, antitussive, expectorant and asthma, or as a substitute for Ganoderma lucidum. Its wild resources are very limited, and so far there is no Successful Artificial Cultivation Methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022]Weigh 75g of peeled and removed potatoes, 6g of sucrose, 6g of agar, and 3g of pine needles. Cut the potatoes into small strips and put them in a pot with pine needles. Add 405g of water and boil for 20 minutes until the potatoes are soft but not rotten. Filter with 6 layers of gauze, take the filtered juice in a pot, add water to make up to the original water volume, add agar to melt, add sucrose and stir evenly, put it in a test tube while it is hot and sterilize, cool to 45°C and pour it into a slope to obtain a slope PDA medium ;

[0023] Weigh 75g of peeled and removed potatoes, 6g of sucrose, 6g of agar, 0.2g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate and VB 1 0.02g, cut the potatoes into small strips and put them in the pot, add 306g of water, boil for 20 minutes until the potatoes are soft but not rotten, filter with 6 layers of gauze, take the filtered juice in the pot, add water to the original water level, add Melt the agar, add sucrose, pot...

Embodiment 2

[0033] Weigh 850g of peeled and removed potatoes, 100g of sucrose, 100g of agar, and 50g of pine needles. Cut the potatoes into small strips and put them into the pot with pine needles. Add 3850g of water and boil for 30 minutes until the potatoes are soft but not rotten. Filter with 8 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, add agar to melt, then add sucrose and stir evenly, put it in a test tube while it is hot and sterilize it, cool it to 50°C and pour it into a slope to obtain slope PDA culture base.

[0034] Weigh 850g of peeled and removed potatoes, 100g of sucrose, 100g of agar, 15g of potassium dihydrogen phosphate, 15g of magnesium sulfate and VB 1 0.06g, cut the potatoes into small strips and put them in the pot, add 4860g of water, boil for 30 minutes until the potatoes are soft but not rotten, filter with 8 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, add Melt th...

Embodiment 3

[0044] Weigh 800g of peeled and removed potatoes, 80g of sucrose, 80g of agar, and 40g of pine needles. Cut the potatoes into small strips and put them into the pot with pine needles. Add 4000g of water and boil for 25 minutes until the potatoes are soft and not rotten. Filter with 7 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, add agar to melt, then add sucrose and stir evenly, put it in a test tube while it is hot, sterilize, cool to 48°C and pour it into a slope, and obtain a slope PDA culture base.

[0045] Weigh 800g of peeled and removed potatoes, 80g of sucrose, 80g of agar, 10g of potassium dihydrogen phosphate, 10g of magnesium sulfate and VB 1 0.04g, cut the potatoes into small strips and put them in the pot, add 3920g of water, boil for 25 minutes until the potatoes are soft but not rotten, filter with 7 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, Add agar to melt, th...

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PUM

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Abstract

The invention relates to a method for artificially cultivating Ganoderma guinanense fruiting bodies, which is characterized by comprising the following steps of: carrying out a tissue separation or a spore separation on fresh Ganoderma guinanense (Ganoderma guinanense J.D.Zhao et Z.Q.Zhang) fruiting bodies; then culturing on a PDA (Potato Dextrose Agar) slant culture medium and a slant comprehensive PDA culture medium to obtain a stock spawn for production; then inoculating the cultured sporophores into a stock culture medium to be cultured; culturing until mature fruiting bodies grow. According to the artificially cultivating method provided by the invention, the biological efficiency of the Ganoderma guinanense fruiting bodies can reach 35-75%, namely 35-75 g of fresh Ganoderma guinanense fruiting bodies can be obtained by utilizing 100 g of culture materials for production; and the fresh Ganoderma guinanense fruiting bodies can be used as raw materials or materials for researching.

Description

technical field [0001] The invention relates to an artificial cultivation method of Ganoderma lucidum fruiting bodies, in particular to an artificial cultivation method of Ganodermaguinanense J.D.Zhao et X.Q.Zhang fruiting bodies. Background technique [0002] Ganoderma lucidum (Curtis) P.Karst. contains Ganoderma lucidum polysaccharides, Ganoderma lucidum polypeptides, triterpenoids, amino acids, proteins, steroids, mannitol, coumarin, alkaloids, organic acids and trace elements Ge, P , Fe, Ca, Mn, Zn, etc., have the functions of tumor inhibition, sedation and analgesia, immune regulation, anti-radiation, liver protection and detoxification, anti-allergic, blood pressure regulation, blood fat and blood sugar lowering, cough and phlegm and asthma resistance, anti-hypoxia Anti-aging and other effects, both medicine and food, and no toxic side effects, so it is favored by consumers, and the market demand is strong. China is rich in species of Ganoderma lucidum, with more than...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H4/00C05G3/00
Inventor 宋斌邹方伦吴兴亮李泰辉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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