Separation and cultivation method for isaria fumosorosea

A method for culturing and a technology for Trichosporium, which are applied in the field of isolation and cultivation of Corynebacterium rosacea strains, can solve the problems of irregular colony growth, spore ejection, inability to measure the diameter of the colony, etc. Avoid the effects of spore shooting and large spore production

Inactive Publication Date: 2013-12-18
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of measuring the biological characteristics, it was found that the traditional method of using a puncher to inoculate the fungus cake would cause the spores to shoot out, the colony would grow irregularly, and the diameter of the colony could not be measured

Method used

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  • Separation and cultivation method for isaria fumosorosea
  • Separation and cultivation method for isaria fumosorosea
  • Separation and cultivation method for isaria fumosorosea

Examples

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Embodiment 1

[0073] Firstly, 5 carcasses of Bemisia tabaci infected by entomogenic fungi were collected from cucumber leaves in the greenhouse, and pathogenic bacteria were isolated from the returned Bemisia tabaci carcasses infested by entomogenic fungi; Sodium hypochlorite solution was used to disinfect the surface of the collected whitefly carcasses, and then rinsed with 10ml of deionized water sterilized at 121°C for 20 minutes for 3 times, put them on the potato dextrose medium (PDA), and placed them upside down in a mold incubator at 25°C (The model of the incubator is MJ-250F-1 mold incubator, produced by Shanghai Yiheng Scientific Instrument Co., Ltd.). After the mycelium is formed, pick the formed mycelium and place it on the potato dextrose medium (PDA) After 10 days, the mycelia on the potato dextrose medium (PDA) gradually formed a dense fluffy white colony, and the back was light yellowish brown. Get the mycelium and put it into a small beaker filled with 10ml0.1% Tween-80 ste...

Embodiment 2

[0077] Firstly, 5 carcasses of Bemisia tabaci infected by entomogenic fungi were collected from cucumber leaves in the greenhouse, and pathogenic bacteria were isolated from the returned Bemisia tabaci carcasses infested by entomogenic fungi; Sodium hypochlorite solution was used to disinfect the surface of the collected whitefly carcasses, and then washed three times with 10ml of deionized water sterilized at 121°C for 20 minutes, put them on the potato sucrose medium (PSA), and placed them upside down in a mold incubator at 25°C (The model of the incubator is MJ-250F-1 mold incubator, produced by Shanghai Yiheng Scientific Instrument Co., Ltd.). After the mycelium is formed, pick the formed mycelium and put it on the potato sucrose medium (PSA) After 10 days, the mycelium on the potato sucrose medium (PSA) gradually formed a dense fluffy white colony, and the back was light yellowish brown. Get the mycelium and put it into a small beaker filled with 10ml0.1% Tween-80 sterile...

Embodiment 3

[0081] Firstly, 4 corpses of Bemisia tabaci infected by entomogenic fungi were collected from cucumber vegetable leaves in the greenhouse, and pathogenic bacteria were isolated from the collected corpses of Bemisia tabaci infected by entomogenic fungi; first, 3% Disinfect the surface of the collected whitefly carcasses with sodium hypochlorite solution, rinse them with 13 ml of deionized water sterilized at 125°C for 15 minutes, put them on the potato dextrose medium (PDA), and place them upside down for mold cultivation at 22°C incubator (the model of the incubator is MJ-250F-1 mold incubator, produced by Shanghai Yiheng Scientific Instrument Co., Ltd.). ), after 12 days, the mycelium on the potato dextrose medium (PDA) gradually formed a dense fluffy white colony, and the back was light yellowish brown. Pick the mycelium and put it into a small beaker filled with 8ml0.1% Tween-80 sterile water, stir with a magnetic stirrer (the magnetic stirrer model is RH basic KT / C, produc...

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Abstract

The invention discloses a separation and cultivation method for isaria fumosorosea. The method comprises the following steps of collecting bemisia tabaci bodies infected with entomogenous fungus from vegetable leaves, separating the entomogenous fungus from the bodies, putting the entomogenous fungus on a PDA (potato dextrose agar) culture medium for cultivating to obtain spore suspension which is sprayed on two-year-old nymphs of bemisia tabaci, then separating the entomogenous fungus from the bodies again after cultivation, storing the entomogenous fungus on a slope of a test tube and storing the test tube in a refrigerator; taking the entomogenous fungus out of the refrigerator, preparing the spore suspension, dipping the spore suspension through filtration paper, inoculating the spore suspension onto the culture medium, cultivating in a mildew incubator, picking up hypha, inoculating the hypha into germfree water, then stirring and filtering; putting the spore suspension at the center of a culture dish, perforating a hole in the center of a bacterial colony, dispersing tween-80, stirring and filtering again, and calculating the sporulation quantity and the diameter of the bacterial colony. The method is used for screening an optimal culture medium formula, the optimal temperature, an optimal pH, a lighting condition and a ventilating condition. The method has the advantages that the diameter area of the bacterial colony is large and the sporulation yield is large.

Description

technical field [0001] The invention relates to a method for isolating and cultivating a bacterial strain, in particular to a method for isolating and cultivating a strain of I. fumigatus. Background technique [0002] I. fumigatus is an important entomogenic fungus, which is widely distributed and can parasitize many harmful insects such as Homoptera, Hemiptera, Diptera, Coleoptera and Hymenoptera. We isolated a The strain of I. fumigatus was named IF-1106. Our research on the biological characteristics of I. fumigatus will help provide a theoretical basis for the mass production of this strain. In the process of measuring the biological characteristics, it was found that the traditional method of using a punch to inoculate the fungus cake would cause the spores to shoot out, the colony would grow irregularly, and the diameter of the colony could not be measured. Therefore, it is necessary to find a suitable culture method to avoid spore ejection, make the colony grow regu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 马瑞燕田晶李新凤梁丽郝赤刘同先
Owner SHANXI AGRI UNIV
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