Method for rejuvenating cordyceps militaris strains

A technology of Cordyceps militaris and strains, applied in botany equipment and methods, horticulture, application, etc., can solve the problems such as degradation of Cordyceps militaris strains, achieve the effects of uniform individual size, easy operation, and reduced deformity rate

Inactive Publication Date: 2011-06-15
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The object of the present invention is to provide a kind of rejuvenation method of Cordyceps militaris bacterial strain, to solve the problems such as degradation of Cordyceps militaris bacterial strain due to repeated passage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Cordyceps militaris ( Cordyceps militaris ) strains, provided free of charge by the Industrial Microbiology Engineering Center of the Ministry of Education.

[0024] 1) Cordyceps militaris ( Cordyceps militaris ) strains were inoculated in PDA slant medium and activated at 25°C;

[0025] PDA slant medium: potato 200g / L, sucrose 20g / L, agar 20g / L, pH 7.0

[0026] 2) The activated Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA plates containing 0.1 mg / ml ampicillin, and rejuvenated at 25°C for 3 days;

[0027] 3) Cordyceps militaris ( Cordyceps militaris ) strains were inoculated again on PDA plates containing 0.3mg / ml ampicillin, and rejuvenated at 25°C for 3 days;

[0028] 4) The rejuvenated Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA slant for strain preservation.

[0029] 5) For rejuvenated Cordyceps militaris ( Cordyceps militaris ) strains were tested for mycelial growth rate, fruiting body productio...

Embodiment 2

[0032] Cordyceps militaris ( Cordyceps militaris ) strain, provided by the Industrial Microbiology Engineering Center of the Ministry of Education.

[0033] 1) Cordyceps militaris ( Cordyceps militaris ) strains were inoculated in PDA slant medium, and activated at 25°C;

[0034] 2) The activated Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA plates each containing 0.2mg / ml kanamycin and ampicillin, and rejuvenated at 25°C for 3 days;

[0035] 3) Cordyceps militaris ( Cordyceps militaris ) strains were re-inoculated on PDA plates containing 1 mg / ml streptomycin, and rejuvenated at 25°C for 3 days

[0036] 4) The rejuvenated Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA slant for strain preservation.

[0037] 5) For rejuvenated Cordyceps militaris ( Cordyceps militaris ) strains were tested for mycelial growth rate, fruiting body production ability and cordycepin production ability.

[0038] 6) The experimental re...

Embodiment 3

[0040] Cordyceps militaris ( Cordyceps militaris ) strains were provided by the Industrial Microbiology Engineering Center of the Ministry of Education.

[0041] 1) Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA slant and activated at 25°C;

[0042] 2) The activated Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA plates each containing 0.025mg / ml micronomycin and cephalosporin IV, and rejuvenated at 25°C for 3 days

[0043] 3) Inoculate the strain after the first rejuvenation on a PDA plate containing 1.0 mg / ml kanamycin + 0.3 mg / ml ampicillin, and carry out rejuvenation culture at 25°C for 3 days;

[0044] 4) The rejuvenated Cordyceps militaris ( Cordyceps militaris ) strains were inoculated on PDA slant for strain preservation.

[0045] 5) The rejuvenated Cordyceps militaris ( Cordyceps militaris ) strains were tested for mycelial growth rate, fruiting body production ability and cordycepin production ability.

[...

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Abstract

The invention relates to rejuvenation of degenerated edible bacterial strains, in particular to a method for rejuvenating cordyceps militaris strains, which mainly comprises steps of cordyceps militaris strain activated culture, cordyceps militaris strain rejuvenation, and cordyceps militaris strain conservation. The method is characterized in that: the rejuvenation culture is that: the activatedcordyceps militaris strain is inoculated to a potato dextrose agar (PDA) plate which is compounded with one or more kinds of antibiotics for first rejuvenation culture, and the strain which is subject to first rejuvenation culture is inoculated to a PDA plating culture medium which is compounded with one or more kinds of antibiotics for second rejuvenation culture. The invention breaks through the traditional strain rejuvenation method, and rejuvenates the strain by an antibiotic method which is easy to operate, simple and practical, and has remarkable effect. The strain which is rejuvenated by the method has robust and compact hypha, and the growing speed of the strain is improved by about 8 times compared with that of the strain which is not rejuvenated.

Description

technical field [0001] The invention relates to the rejuvenation of degenerated edible fungus strains, in particular to a method for rejuvenation of Cordyceps militaris strains. Background technique [0002] Cultivation of edible fungi requires high yield, and the strain is the key. Cordyceps militaris is a model species of Ascomycota, Ergot, Ergotaceae and Cordyceps. scientific name Cordyceps militaris , also known as Cordyceps militaris, Cordyceps militaris, is a close relative of the same genus of Cordyceps sinensis, its effective components are similar to those of Cordyceps sinensis, containing nucleosides, polysaccharides, sterols and S O D enzymes, etc., and the content of cordycepin is higher than that of Cordyceps sinensis has various pharmacological effects such as improving the body's immunity, anti-tumor, anti-aging, protecting heart and lung tissue, sedative and hypnotic. Therefore, Cordyceps militaris is used in health care medicine and food as a subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 毛宁柯轶
Owner FUJIAN NORMAL UNIV
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