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Morchella esculenta bacterial strain and culture method thereof

A cultivation method, the technology of hickory chick, applied in the field of microorganisms, can solve the problems of weak sclerotia generation ability, high pollution rate, uneven mycelium growth, etc., and achieve strong sclerotia generation ability, low culture pollution rate, and high biomass high effect

Active Publication Date: 2014-04-09
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art morel mycelium biomass, weak sclerotia generation ability, uneven mycelial growth, high pollution rate, and insufficient utilization of culture medium.

Method used

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  • Morchella esculenta bacterial strain and culture method thereof
  • Morchella esculenta bacterial strain and culture method thereof

Examples

Experimental program
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Embodiment 1

[0020] A strain of Morchella esculenta (Mochellaesculenta) M-02 provided by the present invention # The acquisition, identification and cultivation methods of strain CGMCC No.7058.

[0021] 1.1 Morchella (Mochellaesculenta) M-02 # Obtaining strain CGMCC No.7058

[0022] (1) Inoculate the collected wild hickory chick sporocarp inner wall tissue block on the slant of the strain separation and purification medium, cultivate at 18°C ​​for 10 days, observe and record the separated and cultured test tubes every day, and remove the contaminated test tubes in time, see Example 4 According to the method, through the comparison of mycelium output, sclerotia production ability, anti-pollution ability and other traits, the strains with high mycelium output, strong sclerotia production ability, pollution resistance and good growth are selected as the obtained morel Bacteria isolation test tube species; the formula of the strain isolation and purification medium is: 30% potato, 2% glucose...

Embodiment 2

[0063] Example 2 is to Morchella esculenta (Mochella esculenta) M-02 # The culture method of bacterial strain CGMCC NO.7058, its culture method is except that the following measures are different, and all the other measures are the same as 1.3 culture method in embodiment 1, and its Morchella esculenta (Mochella esculenta) M-02 # The acquisition, identification and preservation methods of bacterial strain CGMCC No.7058 are identical to those in Example 1.

[0064] 2.1 Culture method

[0065] ⑴Mochella esculenta M-02 # The mycelium of the strain CGMCC No.7058 was inoculated on the slant of the test tube agar medium, and cultured at 20°C for 7 days to obtain the test tube strain.

[0066] (2) Inoculate the test tube bacteria into a 500mL Erlenmeyer flask filled with 200mL of liquid medium, and culture it on a shaker at 20°C with a rotation speed of 130r / min and a culture time of 9 days to obtain a first-class liquid culture. The liquid medium The formula is: 7% bran, 0.5% soy...

Embodiment 3

[0069] Embodiment 3 is to morel (Mochella esculenta) M-02 # The culture method of bacterial strain CGMCC No.7058, its culture method is except that following measure is different, and all the other measures are identical with 1.3 culture method among the embodiment 1, and its Morchella esculenta (Mochella esculenta) M-02 # The acquisition, identification and preservation methods of bacterial strain CGMCC NO.7058 are identical to those in Example 1.

[0070] 3.1 Culture method

[0071] ⑴Mochella esculenta M-02 # The mycelium of the strain CGMCC No.7058 was inoculated on the slant of the test tube agar medium, and cultured at 24°C for 5 days to obtain the test tube strain.

[0072] (2) Inoculate the test tube bacteria into a 500mL Erlenmeyer flask filled with 200mL of liquid medium, and culture it on a shaker at 22°C with a rotation speed of 160r / min and a culture time of 7 days to obtain a first-class liquid culture. The formula is: 10% bran, 1% soybean powder, 1% maltose, 2...

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Abstract

The invention discloses a morchella esculenta bacterial strain and a culture method thereof. The culture method of the morchella esculenta M-02# bacterial strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center ) No.7058 is as follows: inoculating a mycelium of the morchella esculenta bacterial strain into a test tube culture medium inclined surface which contains an improved PDA (Potato Dextrose Agar) culture medium for culturing for 5 days-7 days under 18 DEG C-24 DEG C to obtain a test tube strain; inoculating the test tube strain into a triangular flask which contains a liquid culture medium for culturing on a shaking bed at 20 DEG C-26 DEG C, and culturing for 5 days-7days at rotation speed of 120 r / minute-160r / minute to obtain a primary liquid strain; and inoculating the primary liquid strain into a fermentation tank, ventilating for culturing for 72 hours-96 hours to obtain a liquid strain of the bacterial strain. The bacterial strain disclosed by the invention has mycelium biomass live-weight as high as 25g / kg, strong sclerotium generating capacity, pollution resistance, a low culturing contamination rate not greater than 2%, and short liquid fermentation time of 72 hours-96 hours, so that an excellent strain is provided for morchella esculenta wild resource protection and reproduction promotion.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a new hickory chick strain and a cultivation method thereof. Background technique [0002] Morchella (Morchella) fungus is a world-renowned rare and precious wild edible fungus. Morchella (M.esuclenta (L.) Pers.) is one of the main species of Morchella fungi produced in Yunnan, and it is widely distributed. Occurs in large numbers in the Jinsha River Basin. At present, the artificial cultivation of morel fungi cannot be commercialized and scaled due to the unstable yield, and most of the morel mushrooms on the market come from wild. In recent years, due to adverse factors such as worsening climatic conditions and over-collection, the natural output of wild morels has dropped significantly. Studies have shown that in the original environment of morels, the use of artificial strains and supporting technical measures can effectively increase the output of morels...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 桂明英刘蓓郭相马明邰丽梅吴素蕊
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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