Preparation method and application of zearalenone biodegradation agent

A zearalenone degrading agent, the technology of zearalenone, applied in the application, animal feed, animal feed and other directions

Inactive Publication Date: 2013-07-10
赵刚绩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Zearalenone has an estrogenic effect, can cause acute and chronic poisoning of animals, cause abnormal reproductive function and even death of animals, and can cause huge economic losses to livestock farms
The traditional methods for removing erythralenone mainly include adsorption, extraction, and heat treatment, but they have the disadvantages of time-consuming and laborious, low detoxification rate, and easy loss of nutrients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] The specific operation process of beer yeast degrading erythralenone is as follows:

[0023] Brewer's yeast (CGMCC NO.2.16) was cultured in PDA solid medium at 37°C for 48 hours, a single colony was collected, inserted into 100ml of PDB liquid medium, and cultured at 37°C for 3-5 days. Mix the culture solution with 1kg DDGS, and dry it at 50°C to obtain the crude zearalenone degradation agent, which can be used as a feed additive.

[0024] Take the culture medium, refrigerate and centrifuge at 4°C, 8000r / min for 20 min, take the supernatant, precipitate with 30% ammonium sulfate, refrigerate and centrifuge at 8000r / min for 20 min, take the precipitate, and freeze-dry to obtain Extracted zearalenone degrading enzyme. The refined zearalenone degrading enzyme is mixed with 1% glucose to prepare a refined zearalenone degrading agent.

[0025] Effect:

[0026] 1. In vitro experiment: mix the crude zearalenone degradation agent produced according to this patent with the f...

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PUM

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Abstract

The invention relates to a preparation method of a zearalenone biodegradation agent. The preparation method comprises the following steps: culturing through beer yeast, namely, firstly culturing in a PDA (potato dextrose agar) solid culture medium for 48hours, then taking single colony, inoculating the single colony into a PDB (potato dextrose broth) liquid culture medium for performing shaking culture for 3-5 days, adding 1-10% by weight of yeast fermentation broth by using wine lees protein feed as a carrier to prepare a crude enzyme preparation of the zearalenone biodegradation agent; extracting a zearalenone degradation enzyme of a beer yeast fermentation product, culturing through the beer yeast at the temperature of 37 DEG C for 3-5 days, taking the fermentation broth, performing freezing centrifugation under the condition of 8000r / min at the temperature of 4 DEG C for 20minutes, or separating supernatant liquid from bacterial cells by adopting a suction filtration method; and taking the supernatant liquid which is separated out, thereby obtaining an extracellular crude extraction solution, precipitating with 30-90% of ammonium sulfate, and performing the freezing centrifugation under the condition of 8000r / min at the temperature of 4 DEG C for 20minutes to obtain the zearalenone degradation enzyme which is finely extracted. The zearalenone degradation agent disclosed by the invention has the advantages that yeast can not only degrade toxins, but also adsorb the toxins.

Description

technical field [0001] The invention belongs to the technical field of biological detoxification, and relates to a preparation method and application of a zearalenone biodegradation agent, in particular to the use of brewer's yeast (aspergillus cerevisiae) capable of degrading zearalenone and the degrading enzyme produced by it , to prepare a biological agent for degrading erythralenone, which is used for the biological detoxification of zearalenone, and is mainly used for the degradation of zearalenone in grains such as corn, wheat, rice, barley, millet and oats. Background technique [0002] Zearalenone (Zearalenone), also known as F-2 toxin, was first isolated from corn with head blight. The toxin-producing bacteria of zearalenone are mainly the fungi of the genus Fusarium, such as F. graminearum and F. tricinctum. Zearalenone mainly contaminates grains such as corn, wheat, rice, barley, millet and oats. Among them, the positive detection rate of corn is 45%, and the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16
Inventor 赵刚绩
Owner 赵刚绩
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