Preparation method of trichoderma multifunctional soil modifying agents

Abstract

The invention relates to a preparation method of trichoderma multifunctional soil modifying agents. The preparation method comprises the steps that: trichoderma harzianum SH2303 with the CGMCC (China General Microbiological Culture Collection Center) NO. 4963 is cultured on a potato dextrose agar (PDA) culture medium for 2 to 3 days, a bacterial cake is punched by a hole puncher with the diameter being 5mm, the bacterial cake is inoculated into a seed culture medium for 5 to 7 days at the bacterium inoculation quantity of three tablets per bottle and is then inoculated into a spore production culture medium, the fermentation is carried out for 5 to 7 days under the optimized culture condition in a 300 L fermentation tank, and the number of thick-wall spores is (6 to 7)*10<8> / mL; and fermenting liquid and carriers are mixed according to the weight ratio of 1:1, the secondary open type solid fermentation is carried out for 5 to 7 days at the normal temperature, and the number of trichoderma conidiospores and chlamydospores is 200 to 500 million / g. The soil modifying agents prepared by the method have the main purposes of improving the primary and secondary salinization of vegetable planting soil and degrading the organic phosphorus pesticide residue soil, and have the effects of treating soil-borne diseases, promoting the crop growth and improving the crop yield. Because only one trichoderma strain is utilized, the fermentation process is easy to optimize, the microbial inoculum preparation cost is low, the commercial large-scale popularization is easy to realize, and high field general use values are reached.

Description

technical field [0001] The invention relates to the technical field of biological bacteria soil improver, in particular to a preparation method of Trichoderma multifunctional soil improver. Background technique [0002] According to reports, the area of ​​protected vegetables in my country has reached 50.2 million mu; the output is 168 million tons, accounting for 25% of the total vegetable output in the country; the total output value is more than 410 billion yuan, accounting for 51% of the total vegetable output value. However, soil continuous cropping obstacles have long been the main problem affecting my country's protected vegetables. At present, my country's continuous cropping obstacles are more serious, accounting for about 20-25% of the vegetable planting area, and the losses caused by continuous cropping obstacles are 20-80%. Severe and almost extinct. The main causes of continuous cropping obstacles: ① allelopathy of vegetables and autotoxicity of root exudates. ...

AI Technical Summary

Problems solved by technology

② The multiple cropping index is too high

Excessive continuous cropping or insufficient crop rotation will lead to insufficient decomposition of diseased residues and a large accumulation of pathogens. Once the conditions are suitable, the disease will aggravate

④ Poor fertilizer quality

Primary or secondary salinized soils are difficult for sustainable vegetable production

⑥ Decreased micro-ecological diversity

However, its disadvantages are that the cost is high and the mechanism is not clear

[0005] During the application of soil amendments, there are also some problems that need to be solved urgently: ①The improvement effect of natural amendments is limited, mainly manifested in problems such as short duration or insufficient reserves

②The high cost of artificially synthesized polymer modifiers has the risk of causing secondary pollution to the environment, thus limiting its wide application

③Non-biological soil amendments often have a single function, and the repair effect is not comprehensive or have different degrees of negative effects, etc.

But at present, there are not many Trichoderma soil conditioners with independent intellectual property rights in China.

At present, the better Trichoderma amendment in China, such as Bio-Green Planet soil amendment, is composed of beneficial microorganisms such as Trichoderma and Bacillus, and the number of viable spores is 2 billion / g. It is mainly used for the prevention and control of soil-borne pathogens, and promotes Crop growth, but it does not show the function of reducing salinization and degrading organophosphorus pesticide residues, but its core technology is from the United States, and the product price is relatively high

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