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Method for preparing edible mushroom liquefied strains

A technology of edible fungi and strains, applied in botany equipment and methods, applications, horticulture, etc., to achieve fast growth, low pollution risk, and high efficiency

Active Publication Date: 2013-03-20
TIANSHUI ZHONGXING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defects in the existing production methods of edible fungus strains, the purpose of the invention is to provide a preparation method of edible fungus liquefaction strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the liquefied strain of Flammulina velutipes comprises the following steps:

[0023] Step 1: Purification and cultivation of primary strains

[0024] (1) Preparation of PDA-enriched medium: remove 300g of potatoes, peel them, cut them into small strips, put them in a pot, add 1500ml of water, boil until the potatoes are crispy and not rotten, filter them with 6 layers of gauze to obtain PDA culture base; add 30g of agar to melt, then add 30g of glucose, 1.0g of plant-based peptone, and 3.0g of dry yeast flakes, mix and stir evenly, add water to 1500ml to obtain PDA-enriched medium, and distribute it in 20mm×200mm test tubes while hot; Plug the test tubes with cotton plugs, wrap each of the 7 tubes with kraft paper, and fix them with rubber bands for later use.

[0025] (2) Sterilization and cooling of PDA enriched medium: Put the wrapped test tube into a high-pressure steam sterilizer with a pressure of 1.5kg / m 3 , the temperature was 121°C, ...

Embodiment 2

[0039] The preparation method of the liquefied strain of Pleurotus eryngii comprises the following steps:

[0040] Step 1: Purification and cultivation of primary strains

[0041] (1) Preparation of PDA-enriched medium: remove 300g of potatoes, peel them, cut them into small strips, put them in a pot, add 1500ml of water, boil until the potatoes are crispy and not rotten, filter them with 6 layers of gauze to obtain PDA culture base; add 20g of agar to melt, then add 40g of glucose, 1.2g of peptone, and 2.5g of yeast flakes, mix and stir evenly, replenish water to 1500ml to obtain PDA-enriched medium, and divide it into 20mm×200mm test tubes while hot; plug the test tube Put on tampons, wrap each of 7 with kraft paper, and fix them with rubber bands for later use.

[0042] (2) Sterilization and cooling of PDA enriched medium: Put the wrapped test tube into a high-pressure steam sterilizer with a pressure of 1.4kg / m 3 , the temperature was 122°C, and heated for 30 minutes to ...

Embodiment 3

[0056] The preparation method of the liquefied strain of crab-flavored mushroom comprises the following steps:

[0057] Step 1: Purification and cultivation of primary strains

[0058] (1) Preparation of PDA-enriched medium: remove 300g of potatoes, peel them, cut them into small strips, put them in a pot, add 1200ml of water, boil until the potatoes are crispy and not rotten, filter them with 5 layers of gauze to obtain PDA culture base; add 25g of agar to melt, then add 20g of glucose, 1.5g of peptone, and 2.0g of yeast flakes, mix and stir evenly, replenish water to 1200ml to obtain PDA enriched medium, and divide it into 20mm×200mm test tubes while hot; plug the test tube Put on tampons, wrap each of 7 with kraft paper, and fix them with rubber bands for later use.

[0059] (2) Sterilization and cooling of PDA enriched medium: Put the wrapped test tube into a high-pressure steam sterilizer with a pressure of 1.4kg / m 3 , the temperature was 123°C, and heated for 35 minute...

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Abstract

The invention discloses a method for preparing edible mushroom liquefied strains. The method comprises three steps, i.e. the purification and culture of primary strains, the preparation of sterilized water and the preparation and liquefaction of secondary strains. According to the method, the primary strains are cultured in a PDA (Potato Dextrose Agar)-enriched culture medium so as to obtain purified primary strains; the purified primary strains are segmented and are then inoculated into a water-soluble culture medium so as to obtain the secondary strains; and hyphae of the secondary strains are broken into pieces by using a high-speed strain crusher, and then, the secondary strains are added to the sterilized water and diluted, thereby obtaining the liquefied strains. The method has the advantages of simple process, simplicity and convenience in inoculation, quickness in strain growth, earliness in mushroom growing, good quality, high yield, high efficiency, low pollution risk, low investment and operating cost and good economic benefit. After the edible mushroom liquefied strains prepared by using the method are inoculated, the permeation of the strains is strong, and the strain growth is quick. Furthermore, the culturing and mushroom growing time of the edible mushroom liquefied strains is greatly shortened, the mushroom growing is orderly, the growing speed is high, and the yield is obviously increased.

Description

Technical field [0001] The invention involves the cultivation method of edible bacteria, especially the preparation method of a edible bacteria liquefied bacteria. Background technique [0002] In the production of edible bacteria, the key to success and failure is the production of bacteria, the most investment in production, and people are most worried about the bacteria.The purity, vitality, cultivation time, and anti -miscellaneous ability of the bacteria determine the success or failure of production, the high or quality of the benefits, and the quality of quality.The production of edible bacteria is a key link for the cultivation of edible bacteria. To achieve the high -quality and efficient cultivation of edible fungus cultivation, we must first achieve the high quality, high efficiency and low cost of the bacteria. [0003] The production of edible bacteria in existing technologies mainly includes two production methods: solid bacteria and liquid bacteria. The production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 陶军
Owner TIANSHUI ZHONGXING BIO TECH
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