Preparation method for multifunctional soil remediation microbial inoculum

A soil remediation and multi-functional technology, applied in the restoration of contaminated soil, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as soil pollution, achieve soil activation, environmental protection effects, and rich degradation enzymes Effect

Inactive Publication Date: 2013-02-06
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Claims
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Problems solved by technology

The excessive use of various pesticides and chemical fertilizers has caused multiple pollution to t...

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  • Preparation method for multifunctional soil remediation microbial inoculum
  • Preparation method for multifunctional soil remediation microbial inoculum
  • Preparation method for multifunctional soil remediation microbial inoculum

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preparation example Construction

[0018] The embodiment of the present invention provides a kind of preparation method of multifunctional soil remediation bacterial agent, it comprises:

[0019] A, Aspergillus niger (Aspergillus niger) bacterial strain J6 (preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is: CGMCC NO .4220, storage date October 12, 2010) was cultured to spores by PDA medium (potato glucose agar medium) (components: 200g peeled potato, 20g sucrose, 15-20g agar, natural pH, the same below) After maturity, inoculate in the seed medium, and spores germinate to obtain spore seed liquid;

[0020] B. The spore seed liquid is inoculated in a solid fermentation medium by 5-10% (mass percentage), cultured in a shallow plate at 25-35°C for 4-7d, and turned 2-3 times therebetween to obtain multifunctional soil Repair bacteria. ...

Embodiment 1

[0026]100mg / L phorate aqueous solution is evenly sprayed on 200g sterilization test soil, gets 20g described inoculum inoculation in this test soil and mixes, add sterile water to adjust water content to the soil humidity of 60% (percentage by weight) , put the test soil under the condition of 30 ℃ for cultivation, add sterile water to keep the soil moisture of 60% (percentage by weight) in the cultivation process, take the non-inoculated as the blank control at the same time, take samples every other day and measure formazan by gas chromatography Phosphorus residues. On the 5th day after application, the degradation rate of phorate was 80%.

Embodiment 2

[0028] Select representative fields with convenient transportation, easy observation and management, and require square square, flat surface, uniform fertility, convenient drainage and irrigation, and the planting level is equivalent to the local production level in the suburbs of Jiyuan City in Henan Province where organophosphorus pesticides are widely used wheat field. The test was divided into two groups:

[0029] (1) Control group: conventional fertilization + repeated spraying of the organophosphorus pesticide omethoate (5000mg / L concentration).

[0030] (2) Treatment group: the multifunctional soil remediation bacterial agent treatment group produced by Henan Academy of Sciences Biology Co., Ltd. (conventional fertilization+multiple spraying of organophosphorus pesticide omethoate, its concentration is 5000mg / L,+multifunctional soil remediation The field of bacterial agent) test set 3 repetitions, the treatments were randomly arranged, and the area of ​​the plot was no...

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Abstract

The invention provides a preparation method for multifunctional soil remediation microbial inoculum. The preparation method comprises the following steps: A. cultivating slope culture aspergillus niger strains J6 through a PDA (potato dextrose agar) culture medium until spores are mature, inoculating in a seed culture medium to obtain spore seed liquid after spore germination; and B. inoculating the spore seed liquid in a solid fermentation medium according to mass percent of 5-10%, conducting shallow pan cultivation for 4-7 days at the temperature of 25-35 DEG C, and turning over for 2-3 times to obtain the multifunctional soil remediation microbial inoculum. With the adoption of the multifunctional soil remediation microbial inoculum prepared by the method, organophosphorus pesticide in soil can be efficiently degraded, and heavy metal such as chromium and lead and the like in the soil can be efficiently adsorbed, so that the purpose of multiple remediation of the soil is achieved.

Description

technical field [0001] The invention relates to the technical field of biological fertilizers, in particular to a preparation method of a multifunctional soil repair bacterial agent. Background technique [0002] At present, with the rapid development of industry and agriculture, the problem of soil pollution has become a hot spot of common concern and research in our country and even in the world. The excessive use of various pesticides and chemical fertilizers has caused multiple pollution to the soil; the soil near various chemical plants and mining areas is also seriously polluted by heavy metals. my country is a big country in the production and use of pesticides. The annual pesticide usage is about 800,000 tons, ranking first in the world. The total amount of pesticide production has been ranked second in the world since 1990, second only to the United States. At present, my country's pesticide product structure is still dominated by insecticides, and the production o...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/685
Inventor 谢宝恩王继雯甄静杨文玲刘莹莹李冠杰周伏忠陈国参
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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