Method for preparing active phenolic compounds through rosin-induced bioconversion

A technology of compounds and inducers, which is applied in the fields of fine chemical industry and pharmaceutical biology, can solve the problems of low output value and achieve the effects of high selectivity, low cost and mild reaction conditions

Inactive Publication Date: 2011-09-14
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Method for preparing active phenolic compounds through rosin-induced bioconversion
  • Method for preparing active phenolic compounds through rosin-induced bioconversion
  • Method for preparing active phenolic compounds through rosin-induced bioconversion

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Experimental program
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Effect test

Embodiment 1

[0034] A. The microbial strain A5+ is routinely cultured in PDA medium to obtain activated strains, and then inserted into PDB medium for fermentation for 48 hours; PDA medium is 200g / L potato + 20g / L glucose + 2% agar, pH The value is 6, autoclaved at 121°C for 15 minutes; PDB medium is 200g / L potato + 20g / L glucose, pH value is 6, autoclaved at 121°C for 15min;

[0035] B. Prepare the inducer of the transformation product; the inducer is to add rosin 0.1g in the PDA medium of every 200mL, 5mL of cosolvent 95% ethanol is sampled, and the solution obtained by fully dissolving rosin in ethanol is the inducer;

[0036] C. Add the inducer prepared in step B to the strain fermented in step A, and react for 96 hours at a temperature of 30° C. and a stirring rate of 120 rpm for biotransformation;

[0037] D. The conversion solution of C is filtered and concentrated to obtain the crude extract of the conversion product;

[0038] E. The crude extract of the conversion product is...

Embodiment 2

[0040] A. The microbial strain A5+ is routinely cultured in PDA medium to obtain activated strains, and then inserted into PDB medium for fermentation for 72 hours; PDA medium is 200g / L potato + 20g / L glucose + 1.5% agar, pH The value is 6, autoclaved at 110°C for 20 minutes; PDB medium is 200g / L potato + 20g / L glucose, pH value is 6, autoclaved at 110°C for 30min;

[0041] B. Prepare the inducer of the transformation product; the inducer is to add rosin 0.2g in the PDA medium of every 200mL, 8mL of cosolvent 95% ethanol is sampled, and the solution obtained by fully dissolving rosin in ethanol is the inducer;

[0042] C. Add the inducer prepared in step B to the strain fermented in step A, and react for 72 hours at a temperature of 26° C. and a stirring rate of 128 rpm for biotransformation;

[0043] D. The conversion solution of C is filtered and concentrated to obtain the crude extract of the conversion product;

[0044] E. The crude extract of the conversion product ...

Embodiment 3

[0046] A. The microbial strain A5+ is routinely cultured in PDA medium to obtain activated strains, and then inserted into PDB medium for fermentation for 60 hours; PDA medium is 200g / L potato + 20g / L glucose + 1.8% agar, pH The value is 6, autoclaved at 125°C for 30 minutes; PDB medium is 200g / L potato + 20g / L glucose, pH value is 6, autoclaved at 125°C for 20min;

[0047] B. Prepare the inducer of the transformation product; the inducer is to add rosin 0.3g in the PDA medium of every 200mL, 6mL of cosolvent 95% ethanol is sampled, and the solution obtained by fully dissolving rosin in ethanol is the inducer;

[0048] C. Add the inducer prepared in step B to the strain fermented in step A, and react for 80 hours at a temperature of 29° C. and a stirring rate of 125 rpm for biotransformation;

[0049] D. The conversion solution of C is filtered and concentrated to obtain the crude extract of the conversion product;

[0050] E. The crude extract of the conversion product ...

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Abstract

The invention provides a method for preparing active phenolic compounds through rosin-induced bioconversion. The method comprises the following steps: performing the routine culture of the microbial strain A5<+> in potato dextrose agar (PDA) culture medium to obtain the activated strain, inoculating the activated strain in the PDA culture medium to ferment for 48-72 hours; adding the inducer in the fermented strain to perform bioconversion; filtering and concentrating the conversion fluid to obtain conversion product crude extract; performing gradient alcohol precipitation on the conversion product crude extract, extracting with petroleum ether and ethyl acetate, finally separating and purifying the product through thin layer chromatography, silica gel column chromatography LH-20 gel chromatography and high performance liquid chromatography to obtain O,O-1,1'-di(2-hydroxyl-3,4,5-trimethyl)phenyl oxo bridge and 4,5,6-trimethyl-1,2-pyrocatechol. The bioconversion method has the advantages of high efficiency and selectivity and mild reaction conditions, and can hardly cause environmental pollution; the cost of the method is lower than the extraction method, thus the cost can be saved; and the obtained phenolic compounds have the functions of antioxidant property, antibacterial property, antiviral property, anticancer property, radiation resistance and antiallergic property.

Description

[0001] Field [0002] The invention relates to a method for preparing active phenolic compounds through biotransformation induced by rosin, and belongs to the fields of fine chemical industry and pharmaceutical biotechnology. Background technique [0003] Rosin is a light yellow to yellowish red transparent solid, insoluble in water, but easily soluble in organic solvents, such as acetone, ether, ethanol, ethyl acetate, isopropanol, turpentine, benzene and xylene. Rosin chemical properties: Rosin resin acid contains double chain and carboxyl active gene, has conjugated double bond and typical carboxyl reaction. In addition to being easy to oxidize and isomerize, rosin also has double bond reactions of disproportionation, hydrogenation, addition, and polymerization. At the same time, it also has carboxyl reactions such as esterification, alcoholization, salt formation, decarboxylation, and ammonolysis. The secondary reprocessing of rosin is based on the double bond and carbox...

Claims

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Application Information

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IPC IPC(8): C12P7/22C12R1/645
Inventor 余旭亚王亚明孟庆雄曹建新李巧峰
Owner KUNMING UNIV OF SCI & TECH
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