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39 results about "Phellinus baumii" patented technology

Artificial cultivation method of Phellinus linteus

The invention discloses an artificial cultivation method for Phellinus baumii Pilat. The method comprises the following steps: (1) inoculating the purified strain separated from a Phellinus baumii Pilat fruiting body to a mother seed culture medium so as to obtain a mother seed; (2) inoculating the mother seed to an original seed culture medium so as to obtain a Phellinus baumii Pilat original seed; (3) inoculating the Phellinus baumii Pilat original seed to a cultivar culture medium inside a cultivation bag for culture; and (4) transplanting the cultivation bag fully covered by hypha into a mushroom producing room and carrying out the mushroom producing culture under the following conditions: 85 to 95 percent of relative air humidity and 22 to 25 DEG C of temperature. The method finds out the best environmental factors such as the relative air humidity, the temperature, the illumination, and the like for the growth of the Phellinus baumii Pilat fruiting body. Through the artificial accurate control on the factors of relative humidity and temperature in the mushroom producing room, the method can maximally improve the growth speed of the Phellinus baumii Pilat fruiting body while simultaneously ensuring the quality of the Phellinus baumii Pilat fruiting body. The method has the advantages of high yield, high Phellinus baumii Pilat fruiting body quality, material conservation, short production cycle, and the like.
Owner:BEIJING FORESTRY UNIVERSITY +1

Phellinus baumii fruit body anticancer activity polysaccharide PBPP and preparation method thereof

The invention discloses phellinus baumii fruit body anticancer activity polysaccharide PBPP and a preparation method thereof.The method comprises the steps that crude polysaccharide is extracted from phellinus baumii fruit body powder through ultrasonic wave assisted hot water, precipitation is conducted with ethyl alcohol, acidic polysaccharose components are separated out through DEAE ion exchange chromatography, chromatographic purification is conducted with sephair conditioning unitryl S-100, S-200 and S-400 sequentially, eluent corresponding to eluting peaks is collected, vacuum concentration is conducted, freeze vacuum drying is conducted and a product is obtained.According to the polysaccharide PBPP and the method thereof, the purity is uniform, adverse effects on growth of normal cells do not exist, obvious inhibiting effects on growth of human cervical carcinoma cells HELA and human gastric carcinoma cells SGC-7901 are achieved, and the polysaccharide PBPP can be used for developing anticancer products.
Owner:ZHEJIANG UNIV

Preparation method of Phellinus linteus mycelium

The invention aims at providing a preparation method of a Phellinus linteus mycelium. The method comprises the following steps of: activating Phellinus linteus spawn; executing plate cultivation of potato dextrose agar culture medium; scraping the Phellinus linteus mycelium to inoculate the scraped Phellinus linteus mycelium to the potato dextrose agar culture medium for shake cultivation; collecting the Phellinus linteus mycelium; extracting Phellinus linteus mycelium total RNA; and observing result via agarose gel electrophoresis. The spawn used in the preparation method is the Phellinus linteus spawn (Phellinus baumii Pilate) collected in the CCTCC (China Center for Type Culture Collection), and the collection number is Phellinus linteus DL101 CCTCC M 2011137. The preparation method is simple to operate and has straightforward principle, and a simple and convenient method for obtaining large-scale high-purity Phellinus linteus mycelium and high-quality Phellinus linteus total RNA in the future is provided, and the basis for fundamental research and application development of the Phellinus linteus is also provided.
Owner:NORTHEAST FORESTRY UNIVERSITY

Phellinus igniarius fermentation medium

The invention provides a culture medium for Phellinus igniarius, which comprises the following constituents (by weight percentage): 1950-2050 parts of Syringa amarensis Rupr. wood flour, Chinese chestnut or pear wood flour, 240-260 parts of maize flour, Chinese sorghum bran and / or Chinese sorghum flour, 23-32 parts of gypsum, 5.5-6.5 parts of KH2PO4, 19-21 parts of ammonium sulfate, sucrose or glucose, or 19-21 parts of mannitol and beef grease.
Owner:SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI +1

Preparation method of Phellinus linteus mycelium

The invention aims at providing a preparation method of a Phellinus linteus mycelium. The method comprises the following steps of: activating Phellinus linteus spawn; executing plate cultivation of potato dextrose agar culture medium; scraping the Phellinus linteus mycelium to inoculate the scraped Phellinus linteus mycelium to the potato dextrose agar culture medium for shake cultivation; collecting the Phellinus linteus mycelium; extracting Phellinus linteus mycelium total RNA; and observing result via agarose gel electrophoresis. The spawn used in the preparation method is the Phellinus linteus spawn (Phellinus baumii Pilate) collected in the CCTCC (China Center for Type Culture Collection), and the collection number is Phellinus linteus DL101 CCTCC M 2011137. The preparation method issimple to operate and has straightforward principle, and a simple and convenient method for obtaining large-scale high-purity Phellinus linteus mycelium and high-quality Phellinus linteus total RNA in the future is provided, and the basis for fundamental research and application development of the Phellinus linteus is also provided.
Owner:NORTHEAST FORESTRY UNIVERSITY

Culture method of phellinus baumii mycelia, phellinus baumii beverage and application of phellinus baumii mycelia

The invention discloses a culture method of phellinus baumii mycelia, a phellinus baumii beverage and an application of the phellinus baumii mycelia. The culture method comprises the following steps of (1) performing enlarged culture with a shake flask; (2) performing enlarged culture with a seed tank: inoculating fermentation culture media with shake flask second-class strains cultured and produced in the step (1), and performing enlarged culture so as to obtain first-class phellinus baumii strains; and (3) performing enlarged culture with a fermenter: inoculating the fermenter loaded with the fermentation culture media, with the first-class phellinus baumii strains obtained in the step (2), performing culture with the fermenter, and performing filtration so as to obtain the phellinus baumii mycelia. The phellinus baumii mycelia produced by the method are short in growth cycle and high in quality. Phellinus baumii mycelium powder is prepared through a freeze drying technique, and a beverage prepared based on the phellinus baumii mycelium powder has favorable health-care effects and has advantages of being uniform in beverage color, good in mouth feel, long in storage period and the like.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY

Phellinus baumii MADS-box transcription factor PbMADS1 as well as coding gene and application thereof

ActiveCN113429465AShorten the artificial cultivation cycleFast growthFungiMicroorganism based processesWild typePhellorinia
The invention discloses a phellinus baumii MADS-box transcription factor PbMADS1 as well as a coding gene and application thereof. The amino acid sequence of the transcription factor PbMADS1 is as shown in SEQ ID NO.3, and the sequence of the coding gene is as shown in SEQ ID NO.2. The invention further discloses application of the coding gene in regulation and control of the growth speed of phellinus baumii hyphae. Meanwhile, the invention also discloses an overexpression recombinant vector which comprises a transcription factor PbMADS1 coding gene sequence. Compared with a wild type (WT), the transgenic phellinus baumii strain (T) for expressing the PbMADS1 has the advantages that the mycelial growth speed can be increased by 17.5%, which indicates that the obtained transcription factor PbMADS1 participates in regulation and control of the growth and development of phellinus baumii mycelia, and the application of the transcription factor PbMADS1 in actual production is beneficial to shortening the artificial cultivation period of phellinus baumii.
Owner:HARBIN UNIV

Phellinusbaumii high yield cultivation method

The invention discloses a high yielding cultivation method of Phellinus baumii, and the method is that: first woods are cut into logs and thin branches are made into bundles; the logs and bundles are soaked into water and loaded with strain bags of required specifications, and two ends of the logs and bundles are filled with the mixture of wheat bran and sawdust; hairy fungus is carried out after the processes of sterilization and aseptic inoculation; then strain sticks are transferred into a greenhouse and buried into sand laid on the ground surface; the temperature, sand and air humidity, light transmittance and aeration inside the greenhouse are controlled; after the first-cut is harvested, three batches can be realized under the same cultivation condition. The method has the advantages of great improvement of the fruiting rate, tidiness fruiting, high fruit body yielding with high quality, strong operability and easy popularization.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI

Bacteriostatic protective agent as well as preparation method and application

The invention discloses a bacteriostatic protective agent as well as a preparation method and an application thereof. The preparation method comprises steps as follows: after Phellinus baumii fruiting bodies are crushed, ethyl alcohol is added, and ethyl alcohol extraction is performed; a mixture after extraction with ethyl alcohol is filtered, and residues and an extract liquid are obtained; the extract liquid is concentrated until the ethyl alcohol volatiles completely, and the bacteriostatic protective agent is obtained. The preparation method is convenient to operate and can better meet the requirement of industrial production. The bacteriostatic protective agent prepared with the preparation method is derived from edible fungi, is safe and reliable, has broad spectrum bacteriostatic performance, has no inhibition effect on growth of probiotics, increases the survival rate of beneficial microorganisms while inhibiting harmful microorganisms and has broad market prospect and application value.
Owner:BRIGHT DAIRY & FOOD

Bacteriostatic protective agent as well as preparation method and application

The invention discloses a bacteriostatic protective agent as well as a preparation method and an application thereof. The preparation method comprises steps as follows: Phellinus baumii fruiting bodies are added to water after being crushed, and boiling water extraction is performed; an obtained mixture is filtered, and residues and an extract liquid are obtained; after the extract liquid is concentrated, a supernatant is taken, and a concentrated liquid is obtained; ethyl alcohol is added to the obtained concentrated liquid, standing is performed, precipitates are collected, water is added for dissolution, a supernatant is taken, and the bacteriostatic protective agent is obtained. The preparation method is convenient to operate and can better meet the requirement of industrial production. The bacteriostatic protective agent prepared with the preparation method is derived from edible fungi, is safe and reliable, has broad spectrum bacteriostatic performance, has no inhibition effect on growth of probiotics, increases the survival rate of beneficial microorganisms while inhibiting harmful microorganisms and has broad market prospect and application value.
Owner:BRIGHT DAIRY & FOOD

Ultrafine powder traditional Chinese medicine for treating tumors

InactiveCN102008516BGood effectEnhance the effect of cancer treatmentPowder deliveryKetone active ingredientsBiotechnologyMedicinal herbs
The present invention relates to an ultrafine powder traditional Chinese medicine for treating tumors, which belongs to medicines and drugs. The ultrafine powder traditional Chinese medicine is prepared by adopting 12 fungus Chinese herbal medicines of phellinus chrysoloma, phellinus baumii pilate, jew ear parasitized on Japanese pagodatree, agaricus blazei murill, pleurotus nebrodensis,curcumin, grifola frondosa, cordyceps militaris, umbellate pore furgus, inonotus obliquus, rainbow conk and hedgehog hydnum, wherein the mixture ratio in parts by weight for composition is as follows: phellinus chrysoloma: 40-60 parts; phellinus baumii pilate, jew ear parasitized on Japanese pagodatree, agaricus blazei murill, pleurotus nebrodensis, curcumin, grifola frondosa, cordyceps militaris and umbellate pore furgus: 10-30 parts for each; inonotus obliquus: 5-20 parts; and rainbow conk and hedgehog hydnum: 5-15 parts for each. In the ultrafine powder traditional Chinese medicine, the phellinus chrysoloma and other fungus medicinal materials are utilized as raw materials, and the compatibility principle of monarch, minister, adjuvant and dispatcher of traditional Chinese medicine is adopted, thus the drug effect is obvious, the effect of treating cancers by using the fungus traditional Chinese medicine is enhanced and simultaneously the types of the cancers for fighting against are increased; and the traditional Chinese medicine ultrafine smashing technology is adopted, the drug effect far exceeds the ordinary smashing technology, and especially for the fungus traditional Chinese medicine taking chitin as cell walls, ultrafine smashing is applied to break the cell walls, thus effective substances in cells can be easily absorbed by a human body.
Owner:刘振钦

Artificial cultivation method of Phellinus linteus

The invention discloses an artificial cultivation method for Phellinus baumii Pilat. The method comprises the following steps: (1) inoculating the purified strain separated from a Phellinus baumii Pilat fruiting body to a mother seed culture medium so as to obtain a mother seed; (2) inoculating the mother seed to an original seed culture medium so as to obtain a Phellinus baumii Pilat original seed; (3) inoculating the Phellinus baumii Pilat original seed to a cultivar culture medium inside a cultivation bag for culture; and (4) transplanting the cultivation bag fully covered by hypha into a mushroom producing room and carrying out the mushroom producing culture under the following conditions: 85 to 95 percent of relative air humidity and 22 to 25 DEG C of temperature. The method finds out the best environmental factors such as the relative air humidity, the temperature, the illumination, and the like for the growth of the Phellinus baumii Pilat fruiting body. Through the artificial accurate control on the factors of relative humidity and temperature in the mushroom producing room, the method can maximally improve the growth speed of the Phellinus baumii Pilat fruiting body while simultaneously ensuring the quality of the Phellinus baumii Pilat fruiting body. The method has the advantages of high yield, high Phellinus baumii Pilat fruiting body quality, material conservation, short production cycle, and the like.
Owner:BEIJING FORESTRY UNIVERSITY +1

The application of 3,4-dihydroxyacetophenone in Phylonotus chryseoides against avian influenza h5n1 virus

InactiveCN103816139BClear structureThe technical route is mature and clearAntiviralsKetone active ingredientsH5N1 virusPhellinus igniarius
The invention discloses 3',4'-dihydroxyacetophenone with an avian influenza-resisting activity, which is separated from fermentation broth and fruiting bodies of phellinus igniarius (LexFr) Quel (phellinus linteus (BerketCurt) Teng, phellinus baumii and phellinus hartigii (AlleschetSchnabl) Imaz). The invention firstly discloses a new activity of 3',4'-dihydroxyacetophenone and proves that the 3',4'-dihydroxyacetophenone has an avian influenza-resisting action. Compared with the application of a general compound, the method has the advantages that the special new activity of 3',4'-dihydroxyacetophenone is found, and the 3',4'-dihydroxyacetophenone has the action of resisting the avian influenza virus.
Owner:QINGDAO AGRI UNIV

Normal pressure reverse phase preparation and separating method of dipeptide C8 in phellinus igniarius

InactiveCN103130808AClear structureThe technical route is mature and clearOrganic chemistryDipeptidePyrazine
The invention discloses a separating method of cyclic dipeptide C8 from phellinus igniarius (Phellinus igniarius (LexFr) Quel, phellinusl inteus (BerketCurt) Teng, Phellinus baumii and Phellinushartigii (AlleschetSchnabl) Imaz). The method includes the steps of S1, preparing crude extract of phellinus igniarius, S2, processing the crude extract of phellinus igniarius by means of normal phase silica gel chromatography, S3, processing the obtained product in S2 by means of carbinol gel chromatography, S4, processing the obtained product in S3 by means of normal phase silica gel chromatography for the second time, S5, processing the obtained product in S4 by means of carbinol gel chromatography for the second time, S6, preparing the obtained product in S5 by means of normal pressure reverse phase, and S7, testing the obtained product in S6 by means of high performance liquid chromatography (HPCL) and 1D-HNMR, and thus cyclic dipeptide C8 ((8aS)- hexahydrogen pyrrolo <1,2-a> pyrazine-1,4- diketone) is obtained.
Owner:QINGDAO AGRI UNIV

The application of 1,2-benzenediol in the fungus Phylonotus in resisting avian influenza h5n1 virus

The invention discloses 1,2-benzenediol with an avian influenza-resisting activity, which is separated from fermentation broth and fruiting bodies of phellinus igniarius (LexFr) Quel (phellinus linteus (BerketCurt) Teng, phellinus baumii and phellinus hartigii (AlleschetSchnabl) Imaz). The invention firstly discloses a new activity of 1,2-benzenediol and proves that the 1,2-benzenediol has an avian influenza-resisting action. Compared with the application of a general compound, the method has the advantages that the special new activity of 1,2-benzenediol is found, and the 1,2-benzenediol has the action of resisting the avian influenza virus.
Owner:QINGDAO AGRI UNIV

Application of methyl acetophenone in S. pyogenes against avian influenza h5n1 virus

InactiveCN103816140BHas anti-avian influenza activityNew activityAntiviralsKetone active ingredientsH5N1 virusPhellinus igniarius
The invention discloses methyl acetophenone with an avian influenza-resisting activity, which is separated from fermentation broth and fruiting bodies of phellinus igniarius (LexFr) Quel (phellinus linteus (BerketCurt) Teng, phellinus baumii and phellinus hartigii (AlleschetSchnabl) Imaz). The invention firstly discloses a new activity of methyl acetophenone and proves that the methyl acetophenone has an avian influenza-resisting action. Compared with the application of a general compound, the method has the advantages that the special new activity of methyl acetophenone is found, and the methyl acetophenone has the action of resisting the avian influenza virus; a new idea and a new direction are created for the development and production of drugs for resisting the avian influenza virus.
Owner:QINGDAO AGRI UNIV

Separating technology of aristolane type sesquiterpene in phellinus igniarius

The invention discloses a separating technology of aristolane type sesquiterpene in phellinus igniarius (phellinus igniarius (Le*Fr)Quel, phellinus igniarius (BerketCurt) Teng, phellinus baumii, phellinus hartigii (AlleschetSchnabl)Imaz). The separating technology comprises the following steps of: firstly, preparing phellinus igniarius crude extract; secondly, carrying out positive-phase silica gel chromatography, using chloroform and carbinol to carry out gradient elution; thirdly, carrying out positive-phase silica gel chromatography again and using petroleum ether and acetone as eluants fourthly, carrying out chloroform-carbinol gel chromatography, carbinol gel chromatography and positive-phase silica gel chromatography in sequence; and finally, drying by distillation under reduced pressure to obtain the aristolane type sesquiterpene.
Owner:QINGDAO AGRI UNIV

Application of Cyclic Dipeptide c2 in Phylonotus spp. against Avian Influenza H5N1 Virus

The invention discloses a cyclic dipeptide C2 with an avian influenza-resisting activity, which is separated from fermentation broth and fruiting bodies of phellinus igniarius (LexFr) Quel (phellinus linteus (BerketCurt) Teng, phellinus baumii and phellinus hartigii (AlleschetSchnabl) Imaz). The invention firstly discloses a new activity of the cyclic dipeptide and proves that the cyclic dipeptide has an avian influenza-resisting action. Compared with the application of a general compound, the method has the advantages that the special new activity of the cyclic dipeptide is found, and the cyclic dipeptide has the action of resisting the avian influenza virus.
Owner:QINGDAO AGRI UNIV
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