23 results about "Phellinus baumii" patented technology

The invention relates to the technical field of trace detection, in particular to a rapid nondestructive detection method for phellinus igniarius polysaccharide content based on a near infrared spectrum technology. The method comprises the following steps: acquiring an original spectrum set of phellinus igniarius samples from various sources under a near infrared spectrum, and dividing the samples from the same source into the same group of original spectrum curves; evaluating the stability and distinguishing capability of light absorption change in different wavelength intervals, and obtaining the light absorption credibility of each target wavelength of the original spectrum curve; analyzing the morphological difference of a single curve in each group of spectrum curves relative to the whole group of curves, and obtaining an initial judgment coefficient for curve smoothing processing; and combining the light absorption credibility with the initial judgment coefficient, performing corresponding smoothing processing on each original spectrum curve according to the determined window length and processing order, and analyzing the actual polysaccharide content of the phellinus igniarius sample of the corresponding type. According to the technical scheme, the accuracy of polysaccharide content detection of phellinus igniarius through near infrared spectroscopy is improved.

The invention provides a mulberry phellinus linteus strain SH08, mulberry phellinus linteus polysaccharide as well as a preparation method and application of the mulberry phellinus linteus polysaccharide, and belongs to the technical field of biological medicines and functional foods. The mulberry phellinus igniarius strain SH08 provided by the invention is preserved in the China Center for Type Culture Collection on October 11, 2025, the preservation address is No. 299, eight road, Wuchang District, Wuhan City, Hubei Province, the strain preservation number is CCTCC NO: M20252203, and the mulberry phellinus igniarius strain SH08 is named as SanghuangAKSQSH01. The invention also provides a method for preparing high-purity mulberry phellinus linteus polysaccharide (SHP-PS) from sporocarp of the strain, and structural characterization of the polysaccharide. Experiments prove that the SHP-PS can effectively improve non-alcoholic fatty liver disease (NAFLD) induced by high fat diet, and the mechanism of the SHP-PS is closely related to blood fat regulation, liver injury and inflammation reduction, intestinal barrier repair, intestinal flora structure regulation and short-chain fatty acid generation promotion. The invention provides high-quality raw materials and a clear technical scheme for developing novel medicines or functional foods for preventing and treating NAFLD, and has important application value.

The application provides a detection method of multi-index chemical component content in Phellinus baumii, and relates to the technical field of analysis and detection.The application establishes a method for simultaneously detecting the content of multi-index chemical components (up to 14 kinds) in Phellinus baumii medicinal materials based on HPLC-MS / MS, adopts 70-100% low-grade alcohol as an extraction solvent, detects through HPLC-MS / MS, the response of the chromatographic peak is stronger, and after adding 0.08-0.12 vol% formic acid in the mobile phase, the peak tailing phenomenon can be well improved, and the content of multi-index chemical components in the Phellinus baumii medicinal materials is simultaneously determined.The detection method provided by the application is simple, efficient, high in sensitivity and strong in specificity, has good precision, repeatability and stability, can reflect the content difference of the measured chemical components in different batches of Phellinus baumii medicinal materials, and can provide a reference for the quality evaluation of the Phellinus baumii medicinal materials.

The invention relates to a phellinus igniarius polysaccharide extraction method, which comprises: adopting phellinus igniarius sporocarp, adding a composite extraction solvent, carrying out synchronous enzymatic hydrolysis and extraction with a composite enzyme, carrying out ultrasonic treatment, collecting a filtrate I obtained by filtering the ultrasonic extraction solution, carrying out vacuum concentration on the filtrate I, adding an ethanol solution, mixing, filtering again, and collecting a filter cake and a filtrate II after filtering to obtain the phellinus igniarius polysaccharide. And drying the filter cake under reduced pressure to obtain the phellinus igniarius polysaccharide. The composite extraction solvent is a mixed solution of an ethanol solution, propylene glycol and water according to the volume ratio of (0.4-0.5): (0.1-0.2): (0.3-0.4). The extraction efficiency of phellinus igniarius polysaccharide and phellinus igniarius triterpene active ingredients is effectively improved, the activity and long-term storage stability of the phellinus igniarius polysaccharide are improved, and after the active ingredients are further processed and prepared into the flash release freeze-dried buccal tablets, the disintegration efficiency and the dissolution rate of the buccal tablets are improved, so that the bioavailability of the buccal tablets is improved.

The invention relates to a method for efficiently extracting and preserving uric acid reducing components of phellinus igniarius, and relates to the technical field of phellinus igniarius extraction. The efficient extraction method for the uric acid reducing component of phellinus igniarius comprises the steps of raw material treatment, extraction, purification and concentration and forming. The preservation method of the uric acid reducing component of phellinus igniarius comprises the steps of pretreatment, packaging and storage. According to the application, the natural active structure of flavone can be reserved to the maximum extent, so that the activity of the finally obtained phellinus igniarius flavone powder is more stable.

The application discloses a protein related to synthesis of hispidin of Phellinus baumii, named as SvPKS1, and the protein is any one of the following: A1: a protein with an amino acid sequence as shown in SEQ ID No. 2; A2: a protein with at least 80% identity with the amino acid sequence of SEQ ID No. 2 and with the synthesis activity of hispidin and analogs or derivatives thereof. The application provides the SvPKS1 protein and a coding gene, and the gene is introduced into a coffee acid-producing yeast strain, so that the heterologous synthesis of hispidin can be realized. The application has wide application space and market prospect in the field of synthetic biology of hispidin and derivatives thereof.

The invention discloses an intelligent cultivation method for phellinus igniarius based on environmental parameter regulation and control, and particularly relates to the cross technical field of intelligent agriculture and fungus cultivation, and the method comprises the following steps: constructing a phellinus igniarius growth prediction model; respectively setting differentiated target environment parameter range sets for a mycelium development period, a primordium formation period and a sporocarp growth period; in the cultivation process, environmental data are monitored in real time and input into the model to obtain a predicted growth state, and a regulation and control instruction is generated by comparing the predicted growth state with an expected state, so that environmental parameters are maintained in a target range; when the mycelium reaches physiological maturity, a sporocarp differentiation program is automatically triggered, and an environment target is switched; in the later growth period of the sporocarp, morphological characteristics are analyzed through images and compared with standard morphological characteristics, if the deviation exceeds a threshold value, a parameter optimization instruction is generated, and an environment parameter set for optimizing the growth period of the sporocarp is fed back. Precision, automation and intelligence of phellinus igniarius cultivation are achieved, and the yield and quality are improved.

The utility model discloses a phellinus igniarius cultivation material blending device, and relates to the technical field of phellinus igniarius cultivation auxiliary equipment. Adjusting blocks are fixed to the upper sides of the front side wall and the rear side wall of the partition plate and slidably arranged in sliding grooves in the front side wall and the rear side wall of the feeding box. The limiting screw rods are screwed to the adjusting blocks through threads in a one-to-one correspondence mode, and a plurality of limiting column grooves are formed in the sliding grooves in the front side wall and the rear side wall of the feeding box at equal intervals. The left side wall and the right side wall of the upper surface of the closing plate are slidably arranged in sliding grooves in the inner top wall of the blending barrel through sliding strips. The driving mechanism is arranged in the top wall of the blending cylinder, and the driving mechanism is connected with the two closing plates; the upper end of the stirring rod is rotationally connected with a mounting plate through a bearing; a plurality of stirring blades are fixed on the lower side of the outer ring wall of the stirring rod at equal angles; the rotating mechanism is arranged in the mounting plate and is connected with one end, positioned in the mounting plate, of the stirring rod; the raw materials are accurately proportioned through the adjustable partition plate and the limiting screw rod, synchronous discharging according to the proportion is ensured through linkage control of the sealing plate, and the device is suitable for various blending requirements.

The invention relates to the technical field of medicines, in particular to application of a pharmaceutical composition in preparation of a medicine for treating thalassemia. The invention provides a pharmaceutical composition. The pharmaceutical composition is prepared from the following components in parts by mass: 2 to 5 parts of pravastatin sodium, 1 to 2 parts of roteciterp, 7 to 11 parts of deferasirox, 7 to 11 parts of morroniside and 30 to 40 parts of phellinus igniarius general flavone extract. The pharmaceutical composition provided by the invention shows multiple clinical beneficial effects in treatment of thalassemia. In the aspect of curative effect, the composition can significantly increase the number of peripheral red blood cells, the content of hemoglobin and the hematocrit of the peripheral blood of a patient and effectively reduce the variable coefficient of the volume of the red blood cells, which indicates that the composition not only can improve the anemia state, but also can improve the stability of the quality of the red blood cells. Besides, the traditional Chinese medicine composition can obviously relieve spleen enlargement and iron overload degree and reduce the iron deposition level of the liver and the spleen, so that the traditional Chinese medicine composition has a positive effect in the aspects of relieving hemolysis related complications and organ iron toxicity.

The application discloses a selenium-rich Phellinus baumii strain domestication, cultivation and selenium enrichment method, which follows the gradient domestication principle of low-selenium starting and gradual increase, sequentially completes selenium source domestication of the Phellinus baumii primary mother strain, secondary original strain and tertiary cultivation strain, and obtains the cultivation strain suitable for the high-selenium environment; then, the tertiary cultivation strain is taken as the inoculation source, standardized cultivation is carried out by adopting the bagged solid culture medium, and the temperature, humidity, light and air conditions are regulated and controlled in two stages of mycelium culture and fruiting body growth management; and through gradient selenium source supply, continuous selenium supplement during the cultivation period, accurate regulation and control of the environmental conditions and scientific treatment after harvesting, efficient absorption and conversion of selenium by the Phellinus baumii are realized, inorganic selenium is converted into organic selenium, and the organic selenium is enriched in the fruiting body. The application solves the problems of low selenium tolerance and poor selenium enrichment efficiency of the Phellinus baumii, the selenium-rich Phellinus baumii fruiting body has high and stable organic selenium content, and the strain domestication success rate and cultivation survival rate are greatly improved.

The invention provides a preservation method of an edible and medicinal fungus strain. The preservation method comprises the following specific steps: (1) culturing a strain in a plate until the diameter of a bacterial colony reaches 3-5cm; (2) picking a strain block at the edge of a bacterial colony, and inoculating the strain block into a cryopreservation tube; (3) adding a protective agent to completely submerge the strain blocks; (4) putting the cryopreservation tube into a programmed cooling box, putting the programmed cooling box into a refrigerator with the temperature of-80 DEG C, and moving the cryopreservation tube into a cryopreservation box after cooling is completed; and (5) taking the preserved strain, heating the cryopreservation tube in a water bath, and culturing in a plate filled with the activated culture medium, so that the vitality of the strain can be recovered. The method is easy to operate, low in cost, safe, effective and suitable for medium-and-long-term preservation of edible and medicinal fungus strains such as grifola frondosa, phellinus igniarius, antrodia camphorata, lepista sordida and black fungus.

The invention relates to the technical field of medical health care products, in particular to an anticancer composition containing phellinus igniarius extract and total ginsenoside and application of the anticancer composition. The anticancer composition comprises a phellinus igniarius extract and total saponins of ginseng. The mass ratio of the phellinus igniarius extract to the total ginsenoside is 1: (0.5-2); the phellinus igniarius extract is obtained by taking artificially cultivated phellinus igniarius as a raw material through ultrasonic-assisted extraction and reflux extraction in stages; the extraction solvent is selected from water or an ethanol water solution with the ethanol concentration not exceeding 40%. The anticancer composition provided by the invention has remarkable in-vitro anticancer activity and low toxicity to normal cells.

This invention relates to a method for cultivating Phellinus linteus, and pertains to the field of Phellinus linteus cultivation technology. The method includes substrate preparation, inoculation and mycelial cultivation, cultivation management, and collection steps. This invention employs an organic cultivation method where yellowing occurs directly at the inoculation site. Utilizing the natural high-oxygen channel at the inoculation site, mycelia are directed and enriched along the path of least resistance, promoting rapid differentiation of Phellinus linteus primordia at the site and direct entry into normal growth. This results in uniform and timely yellowing, further improving the yield and quality of Phellinus linteus. Simultaneously, the overall cultivation cycle is controllable, making it suitable for large-scale, efficient production of organic Phellinus linteus.

The invention discloses a selenium source regulation and control method for directionally producing high-value selenium-rich phellinus igniarius mycelium or sporocarp, and belongs to the technical field of edible and medicinal fungus cultivation and microbial fermentation. The invention provides a target-oriented differential selenium source strategy aiming at the technical contradiction that a single selenium source cannot meet the requirements of different growth stages of phellinus igniarius in the prior art. When the phellinus igniarius mycelium is used as a target product, a fermentation strategy of adding selenium-enriched yeast in the whole process is adopted; when phellinus igniarius sporocarp is used as a target product, a staged strategy is adopted: selenium-enriched yeast is added in a mycelium culture stage, and sodium selenite is added in a sporocarp culture stage. According to the method, the rule that the mycelium stage is adaptive to organic selenium and the sporocarp stage is adaptive to inorganic selenium is defined for the first time, and through accurate stage-selenium source matching, the selenium content, yield and comprehensive quality of the mycelium or the sporocarp are synchronously improved successfully on the premise that mycelium growth is hardly inhibited.

The application discloses an integrated cultivation management system and method for Phellinus baumii, relates to the technical field of integrated cultivation management, and comprises the following steps: based on cultivation environment and growth data set, a nonlinear relationship between environmental characteristics and growth characteristics is extracted through a data mining method, and a multi-factor influence regression and prediction model is constructed; the influence degree of the environmental characteristics on the growth of Phellinus baumii is evaluated through the multi-factor influence regression and prediction model, key influencing factors are identified, and an environmental adjustment suggestion is generated; the cultivation environment is adjusted according to the environmental adjustment suggestion, the adjustment range and frequency are optimized in combination with Phellinus baumii cultivation environment data, and an optimized cultivation environment is generated; based on the optimized cultivation environment, the colonization of Phellinus baumii mycelium and the development of fruiting bodies are measured, the effect of the current cultivation environment is evaluated through statistical regression, and a cultivation management evaluation report is generated. The application optimizes the growth conditions of Phellinus baumii, and improves the cultivation efficiency and yield.

The present invention discloses a phellinus igniarius strain liquid, and relates to the technical field of phellinus igniarius cultivation, the phellinus igniarius strain liquid comprises a PDB culture solution and a water-soluble growth promoting component, the PDB culture solution contains 4.0 g / L of potato extract and 20.0 g / L of glucose, and the water-soluble growth promoting components comprise sodium salicylate and nicotinamide mononucleoside, and the weight ratio of the sodium salicylate to the nicotinamide mononucleoside is 0.005%-0.3% and 0.005%-0.03%. The invention further provides a liquid cultivation method of the phellinus igniarius strain. According to the invention, water-soluble growth promoting components of sodium salicylate and nicotinamide mononucleoside are added on the basis of a PDB culture solution formula, so that the hypha growth speed and the hypha quantity can be remarkably promoted, meanwhile, the hypha activity is improved, the hypha growth activity reduction caused by long hypha growth period is avoided, and the phellinus igniarius liquid strain quality is improved.

The preparation method comprises the following steps: taking a proper amount of dried phellinus igniarius powder, further drying the phellinus igniarius powder in a dryer until the weight of the phellinus igniarius powder is constant, removing residual moisture, and sieving the dried phellinus igniarius powder; adding the pretreated phellinus igniarius powder into an extraction solvent according to the mass ratio of 1: 10, uniformly mixing, putting the mixed solution into an ultrasonic extractor, and setting the ultrasonic frequency to be 40kHz, the power to be 200W and the extraction time to be 30 minutes; filtering the mixed solution subjected to ultrasonic-assisted extraction, removing solid residues to obtain a primary extract, transferring the primary extract into a supercritical fluid extraction device, selecting supercritical fluid as an extraction agent, and setting the temperature of the supercritical fluid to be 40 DEG C, the pressure to be 25MPa and the extraction time to be 60 minutes; and after the extraction is finished, collecting an extracting solution, removing residual solid particles through a filtering device, and concentrating the extracting solution by using a concentrating device to obtain a concentrated extract.

The application discloses a method for extracting phenolic active substances from Phellinus baumii fruiting bodies by adopting a series extraction and purification method and application of the phenolic active substances in uric acid regulation, and belongs to the technical field of deep processing of edible fungi. The method is as follows: after the Phellinus baumii fruiting bodies are soaked in an acetone solution, a crude acetone extract is obtained by using a multi-frequency ultrasonic device, then the crude acetone extract is dissolved in ethanol, and a HPD-826 macroporous resin filler and a LX-17 macroporous resin filler are connected in series to carry out adsorption and elution, so that a pure Phellinus baumii polyphenol extract is obtained. The polyphenol content of the phenolic active substances prepared from the Phellinus baumii fruiting bodies is as high as 80% or more, main active substances are protocatechuic acid, protocatechuic aldehyde, coffee acid, p-coumaric acid, dihydroquercetin, Hispidin and the like, and the phenolic active substances can be prepared into medicines for regulating intestinal flora structure and uric acid metabolism.

The present application belongs to the technical field of fungus cultivation, and particularly relates to a cultivation method of high-yield and high-quality Phellinus baumii. The cultivation method comprises the following steps: (1) inoculation: preparing a culture medium, filling the culture medium into a plastic bag, sterilizing and cooling, and inoculating with Phellinus baumii spores; (2) fungus management: avoiding light cultivation of the inoculated plastic bag; when the fungus management is performed for 30-35 days, the fungus bag is longitudinally squeezed and shortened, and the cultivation is continued to enter the fruiting management; (3) fruiting management: transferring the matured Phellinus baumii fungus bag into a fruiting greenhouse, drawing a rectangular fruiting opening in the middle of the fungus bag, and performing the Phellinus baumii fruiting management; (4) harvesting: obtaining the Phellinus baumii fruiting body after 100-120 days after the opening is drawn. The present application provides a cultivation method of high-yield and high-quality Phellinus baumii, which significantly improves the biological conversion rate and effective component content of Phellinus baumii by optimizing the culture medium components and ratio, and solves the technical problems of low yield and low effective component content in traditional cultivation.

The application relates to application of Phellinus baumii exosomes in glioma treatment, an extraction method and a composition. Phellinus baumii is used as raw material, Phellinus baumii exosomes are provided through operations such as soaking, crushing and centrifugation, and it is verified that the Phellinus baumii exosomes have anti-glioma activity in vitro. Based on the research result, the application provides a new application of the plant exosomes from Phellinus baumii for glioma treatment, and provides a cost-effective potential active ingredient for glioma drug development.

The present application relates to the technical field of bioinformatics, and particularly relates to a method for protecting and developing and utilizing Phellinus baumii germplasm resources, obtaining a preset number of germplasm resources associated with picking cycles and picking frequencies of Phellinus baumii in each region, and characteristic information of Phellinus baumii in each region; setting any Phellinus baumii as an effective Phellinus baumii, obtaining a matching degree value of the effective Phellinus baumii and other Phellinus baumii in each region according to the difference between the same strain characteristic information of the effective Phellinus baumii and other Phellinus baumii, screening out candidate Phellinus baumii of the effective Phellinus baumii, and obtaining an adjustment application value score confidence of the effective Phellinus baumii associated with each germplasm resource; adjusting a cost function of an associated latent semantic model according to the adjustment application value score confidence of the effective Phellinus baumii associated with each germplasm resource in each region, obtaining a predicted application value matrix, and adjusting protection strategy information associated with each germplasm resource. The present application can accurately adjust the protection strategy information of the germplasm resources, and improve the mature utilization rate of the germplasm resources.