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Normal pressure reverse phase preparation and separating method of dipeptide C8 in phellinus igniarius

A Phellinus and cyclic dipeptide technology, applied in the direction of organic chemistry, etc., to achieve the effects of maturity, clear technical route, and clear structure

Inactive Publication Date: 2013-06-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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The effect of this compound has not yet been reported

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  • Normal pressure reverse phase preparation and separating method of dipeptide C8 in phellinus igniarius
  • Normal pressure reverse phase preparation and separating method of dipeptide C8 in phellinus igniarius

Examples

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example 1

[0030] Phellinus linteus crude extract is obtained by the following method:

[0031] (1) The formula of the fermentation medium in grams / 100 ml is:

[0032] Corn Starch 1% Glucose 1%

[0033] Peptone 0.1% Yeast extract 0.1%

[0034] Magnesium sulfate 0.1% Potassium dihydrogen phosphate 0.01%

[0035] (2) Phellinus linteus species is inoculated in the Erlenmeyer flask equipped with liquid culture medium with a conventional method, with a temperature of 25 ° C, the rotating speed of the shake flask is 110r / min, and under the condition of pH 7, the shaking culture is carried out for 7 days; When the value drops to 3, the seeds in the shake flask are inoculated into the culture solution of the 50L fermenter, and the temperature is 25°C, the pressure of the fermenter is 0.1kg / cm 2 , pH 3, the ventilation rate is 0.5-1.1vvm, and the stirring speed is 100 rpm. Under the condition of / min, after culturing for 7 days, the whole liquid fermentation of Phellinus linteus mycelium can b...

example 2

[0042] Phellinus linteus crude extract is obtained by the following method:

[0043] (1) The formula of the fermentation medium in grams / 100 ml is:

[0044] Cornstarch 3% Dextrose 2%

[0045] Peptone 0.5% Yeast extract 0.5%

[0046] Magnesium sulfate 0.5% Potassium dihydrogen phosphate 0.05%

[0047](2) Phellinus linteus species is inoculated into the Erlenmeyer flask equipped with liquid culture medium by a conventional method, at a temperature of 30 ° C, the rotating speed of the shake flask is 180 r / min, and under the condition of pH 6, the shaking culture is carried out for 15 days; When it drops to 2.5, the seeds in the shake flask are inoculated into the culture solution of the 50L fermentor, and the temperature is 30°C, the pressure of the fermenter is 0.2kg / cm 2 , pH 3, the ventilation rate is 0.5-1.1vvm, and the stirring speed is 180 rpm / Under different conditions, cultivated for 15 days, the whole liquid of Phellinus linteus mycelium fermentation can be used to p...

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Abstract

The invention discloses a separating method of cyclic dipeptide C8 from phellinus igniarius (Phellinus igniarius (LexFr) Quel, phellinusl inteus (BerketCurt) Teng, Phellinus baumii and Phellinushartigii (AlleschetSchnabl) Imaz). The method includes the steps of S1, preparing crude extract of phellinus igniarius, S2, processing the crude extract of phellinus igniarius by means of normal phase silica gel chromatography, S3, processing the obtained product in S2 by means of carbinol gel chromatography, S4, processing the obtained product in S3 by means of normal phase silica gel chromatography for the second time, S5, processing the obtained product in S4 by means of carbinol gel chromatography for the second time, S6, preparing the obtained product in S5 by means of normal pressure reverse phase, and S7, testing the obtained product in S6 by means of high performance liquid chromatography (HPCL) and 1D-HNMR, and thus cyclic dipeptide C8 ((8aS)- hexahydrogen pyrrolo <1,2-a> pyrazine-1,4- diketone) is obtained.

Description

technical field [0001] The invention belongs to the field of bioengineering pharmacy. Background technique [0002] Phellinus, fruiting body sessile, cap flat hemispherical or horseshoe-shaped, 2-12*3-21 cm, 1.5-10 cm thick, woody, light liver brown to dark gray or black, often cracked in old age, without skin, There are fine hairs at the beginning, and then become glabrous, with concentric ring edges. The edges are blunt, dark cinnamon to light brown, and there is no fruit layer on the lower side. The flesh is dark brown, hard, and woody. Layer, but the layer is not obvious, the old tube layer is full of white hyphae. The tube mouth is rusty brown to maroon, round, 4-5 per mm. The spores are nearly spherical, smooth, colorless, 5-6*3- 4 microns. The top of the bristles is sharp, the base is enlarged, 10-25*5-7 microns. The hyphae are unbranched, without transverse septum, 3-5 microns in diameter. [0003] At present, there are many purification methods for fungal products...

Claims

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Application Information

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IPC IPC(8): C07D487/04
Inventor 赵晨宋爱荣孙效乐陈师勇王进平
Owner QINGDAO AGRI UNIV
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