A kind of antibacterial protective agent and its preparation method and application

A protective agent and dairy product technology, which is applied in the field of antibacterial protective agent and preparation, can solve the problems of lack of broad-spectrum antibacterial property and small action range, and achieve the effect of broad market application value, protection of survival rate, and easy operation

Active Publication Date: 2019-10-25
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to solve the defects that the exogenous antibacterial components currently used in dairy products have no broad-spectrum antibacterial properties, a small range of action, and few types that can ensure safety and non-toxicity. The present invention provides A bacteriostatic protective agent and its preparation method and application are disclosed. The bacteriostatic protective agent is derived from microorganisms, has a wide range of action and broad-spectrum antibacterial properties, is natural, safe, and has a simple and reliable preparation method, and is suitable for large-scale production and in dairy products. product market application

Method used

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  • A kind of antibacterial protective agent and its preparation method and application
  • A kind of antibacterial protective agent and its preparation method and application
  • A kind of antibacterial protective agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0033] Example 2 Inhibition of bacteriostatic protective agent on Gram-positive bacteria

[0034] After cultivating the Phellinus igniarius strains to obtain the fruiting bodies, pulverize the fruiting bodies, and add 30 times the volume of 70% ethanol to the dried pulverized fruiting bodies for extraction; after 12 hours of static extraction at room temperature, the fruiting bodies of Phellinus igniarius are soaked in ethanol The mixture is filtered, and the above steps are repeated for the second extraction of the residue obtained from the filtration; the two ethanol extracts are combined and concentrated under reduced pressure. After the ethanol is completely volatilized, it is freeze-dried to obtain the ethanol extract of Phellinus igniarius fruiting bodies. Bacteriostatic protective agent; the ethanol extract of Phellinus igniarius was dissolved in ethanol to prepare the alcohol extract solution of Phellinus igniarius with concentrations of 0.01mg / mL, 0.1mg / mL, 1mg / mL, 5mg / mL...

Embodiment 3

[0039] Example 3 Inhibition of fungi by antibacterial protective agent

[0040] After the fruit bodies obtained by cultivating the Phellinus linteus strains, the fruit bodies are crushed, and 20 times the volume of 95% ethanol is added to the dried fruit body pulverized material for extraction; after being allowed to stand at room temperature for 24 hours, the Phellinus linteus seeds are soaked in ethanol The mixture obtained by the entity is filtered, and the above steps are repeated for the second extraction of the filtered residue; the two ethanol extracts are combined, and concentrated under reduced pressure. After the ethanol is completely volatilized, freeze-drying is performed to obtain the Phellinus linteus fruiting body ethanol The extract is the antibacterial protective agent.

[0041] Preparation of solid PDA medium: Take 4 g of PDA agar powder, add 100 mL of deionized water to dissolve it, sterilize at 121°C for 20 minutes, and set aside.

[0042] Take Penicillium candid...

Embodiment 4

[0045] Example 4 Inhibition of Bacteriostatic Protectant on Mold

[0046] After the fruit body obtained by cultivating the Phellinus igniarius strain, the fruit body is dried, the dried fruit body is pulverized, and 20 times the volume of 95% ethanol is added to the dried fruit body pulverized material for extraction; standing at room temperature for extraction After 24h, filter the mixture obtained by soaking Phellinus linteus fruiting bodies in ethanol, repeat the above steps to extract the residue obtained from the filtration for the second time; combine the two ethanol extracts and concentrate under reduced pressure. After the ethanol is completely volatilized, proceed Freeze-drying, the ethanol extract of Phellinus igniarius fruiting body, namely the antibacterial protective agent.

[0047] The same mold inhibition experiment as in Example 3 was carried out, and it was found that when the bacteriostatic protective agent of the present invention exists, the diameter of the mold...

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Abstract

The invention discloses a bacteriostatic protective agent as well as a preparation method and an application thereof. The preparation method comprises steps as follows: after Phellinus baumii fruiting bodies are crushed, ethyl alcohol is added, and ethyl alcohol extraction is performed; a mixture after extraction with ethyl alcohol is filtered, and residues and an extract liquid are obtained; the extract liquid is concentrated until the ethyl alcohol volatiles completely, and the bacteriostatic protective agent is obtained. The preparation method is convenient to operate and can better meet the requirement of industrial production. The bacteriostatic protective agent prepared with the preparation method is derived from edible fungi, is safe and reliable, has broad spectrum bacteriostatic performance, has no inhibition effect on growth of probiotics, increases the survival rate of beneficial microorganisms while inhibiting harmful microorganisms and has broad market prospect and application value.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a bacteriostatic protective agent, a preparation method and an application. Background technique [0002] At present, the use of natural substances for food preservation has reached 25% of the market share and is increasing at an annual rate of 6%-10%. 25% of the world's food supply is contaminated by microorganisms. Although our country is more and more open to food laws and regulations, its requirements for food safety are becoming more and more stringent. With the improvement of people's consumption level and awareness, natural and safe food has become the main consumption trend of consumers. The natural antibacterial components are mainly divided into animal-derived antibacterial components, plant-derived antibacterial components and microbial antibacterial components. Due to the many advantages of microbial bacteriostatic agents, such as easy availability, wide sources,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C3/08
CPCA23C3/08
Inventor 王钦博杨焱杭锋刘振民齐晓彦穆海菠王国骄洪青雍靖怡
Owner BRIGHT DAIRY & FOOD CO LTD
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