A kind of antibacterial protective agent and its preparation method and application
A protective agent and dairy product technology, which is applied in the field of antibacterial protective agent and preparation, can solve the problems of lack of broad-spectrum antibacterial property and small action range, and achieve the effect of broad market application value, protection of survival rate, and easy operation
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Embodiment 2
[0033] Example 2 Inhibition of bacteriostatic protective agent on Gram-positive bacteria
[0034] After cultivating the Phellinus igniarius strains to obtain the fruiting bodies, pulverize the fruiting bodies, and add 30 times the volume of 70% ethanol to the dried pulverized fruiting bodies for extraction; after 12 hours of static extraction at room temperature, the fruiting bodies of Phellinus igniarius are soaked in ethanol The mixture is filtered, and the above steps are repeated for the second extraction of the residue obtained from the filtration; the two ethanol extracts are combined and concentrated under reduced pressure. After the ethanol is completely volatilized, it is freeze-dried to obtain the ethanol extract of Phellinus igniarius fruiting bodies. Bacteriostatic protective agent; the ethanol extract of Phellinus igniarius was dissolved in ethanol to prepare the alcohol extract solution of Phellinus igniarius with concentrations of 0.01mg / mL, 0.1mg / mL, 1mg / mL, 5mg / mL...
Embodiment 3
[0039] Example 3 Inhibition of fungi by antibacterial protective agent
[0040] After the fruit bodies obtained by cultivating the Phellinus linteus strains, the fruit bodies are crushed, and 20 times the volume of 95% ethanol is added to the dried fruit body pulverized material for extraction; after being allowed to stand at room temperature for 24 hours, the Phellinus linteus seeds are soaked in ethanol The mixture obtained by the entity is filtered, and the above steps are repeated for the second extraction of the filtered residue; the two ethanol extracts are combined, and concentrated under reduced pressure. After the ethanol is completely volatilized, freeze-drying is performed to obtain the Phellinus linteus fruiting body ethanol The extract is the antibacterial protective agent.
[0041] Preparation of solid PDA medium: Take 4 g of PDA agar powder, add 100 mL of deionized water to dissolve it, sterilize at 121°C for 20 minutes, and set aside.
[0042] Take Penicillium candid...
Embodiment 4
[0045] Example 4 Inhibition of Bacteriostatic Protectant on Mold
[0046] After the fruit body obtained by cultivating the Phellinus igniarius strain, the fruit body is dried, the dried fruit body is pulverized, and 20 times the volume of 95% ethanol is added to the dried fruit body pulverized material for extraction; standing at room temperature for extraction After 24h, filter the mixture obtained by soaking Phellinus linteus fruiting bodies in ethanol, repeat the above steps to extract the residue obtained from the filtration for the second time; combine the two ethanol extracts and concentrate under reduced pressure. After the ethanol is completely volatilized, proceed Freeze-drying, the ethanol extract of Phellinus igniarius fruiting body, namely the antibacterial protective agent.
[0047] The same mold inhibition experiment as in Example 3 was carried out, and it was found that when the bacteriostatic protective agent of the present invention exists, the diameter of the mold...
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