Phellinus baumii fruit body anticancer activity polysaccharide PBPP and preparation method thereof

An anti-cancer activity, the technology of Phellinus radix, applied in the direction of anti-tumor drugs, drug combinations, etc., can solve problems such as hindering medicinal value and unclear medicinal mechanism, and achieve the effect of improving application value and high purity

Active Publication Date: 2016-07-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional Chinese medicine believes that Phellinus can benefit the five internal organs, soften and harden, detoxify, stop bleeding, etc. Modern medical research has found that Phellinus has various pharmacological effects such as

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take the phellinus fruiting body powder obtained by the above method through a 80-mesh sieve, add double distilled water according to the mass-volume ratio of material to liquid at 0.04g / mL, after ultrasonic treatment for 35min, extract at 100°C for 4.35h, then suction filter, repeat Once, combine the filtrate obtained by two suction filtrations, concentrate under reduced pressure at 45°C to 1 / 4 of the original volume, and extract the crude polysaccharide;

[0032] Add absolute ethanol to the crude polysaccharide solution, stir while adding, until the ethanol concentration reaches 80%, put in a refrigerator at 4°C overnight, and centrifuge at 10,000rpm for 10min to obtain precipitated polysaccharide.

[0033] Dissolve the precipitated polysaccharide in double distilled water to prepare a 10mg / mL solution, centrifuge at 10000rpm for 10min, take the supernatant, pass it through a 0.22μm filter membrane, put it on a DEAE-Sepharose ion exchange chromatography column, and use...

Embodiment 2

[0039] Take the Phellinus Phellinus fruiting body powder obtained by the above method through a 90-mesh sieve, add double distilled water according to the mass-volume ratio of material to liquid at 0.04g / mL, after ultrasonic treatment for 35min, extract at 100°C for 4.35h, then suction filter, repeat Once, combine the filtrate obtained by two suction filtrations, concentrate under reduced pressure at 45°C to 1 / 4 of the original volume, and extract the crude polysaccharide;

[0040] Add absolute ethanol to the crude polysaccharide solution, stir while adding, until the ethanol concentration reaches 80%, put in a refrigerator at 4°C overnight, and centrifuge at 10,000rpm for 10min to obtain precipitated polysaccharide.

[0041]Dissolve the precipitated polysaccharide in double distilled water to prepare a 10mg / mL solution, centrifuge at 10000rpm for 10min, take the supernatant, pass it through a 0.22μm filter membrane, put it on a DEAE-Sepharose ion exchange chromatography column...

Embodiment 3

[0047] Take the phellinus fruiting body powder obtained by the above-mentioned method through a 100-mesh sieve, add double distilled water according to the mass-volume ratio of the material to liquid at 0.04g / mL, after ultrasonic treatment for 35min, extract at 100°C for 4.35h, then suction filter, repeat Once, combine the filtrate obtained by two suction filtrations, concentrate under reduced pressure at 45°C to 1 / 4 of the original volume, and extract the crude polysaccharide;

[0048] Add absolute ethanol to the crude polysaccharide solution, stir while adding, until the ethanol concentration reaches 80%, put in a refrigerator at 4°C overnight, and centrifuge at 10,000rpm for 10min to obtain precipitated polysaccharide.

[0049] Dissolve the precipitated polysaccharide in double distilled water to prepare a 10mg / mL solution, centrifuge at 10000rpm for 10min, take the supernatant, pass it through a 0.22μm filter membrane, put it on a DEAE-Sepharose ion exchange chromatography ...

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PUM

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Abstract

The invention discloses phellinus baumii fruit body anticancer activity polysaccharide PBPP and a preparation method thereof.The method comprises the steps that crude polysaccharide is extracted from phellinus baumii fruit body powder through ultrasonic wave assisted hot water, precipitation is conducted with ethyl alcohol, acidic polysaccharose components are separated out through DEAE ion exchange chromatography, chromatographic purification is conducted with sephair conditioning unitryl S-100, S-200 and S-400 sequentially, eluent corresponding to eluting peaks is collected, vacuum concentration is conducted, freeze vacuum drying is conducted and a product is obtained.According to the polysaccharide PBPP and the method thereof, the purity is uniform, adverse effects on growth of normal cells do not exist, obvious inhibiting effects on growth of human cervical carcinoma cells HELA and human gastric carcinoma cells SGC-7901 are achieved, and the polysaccharide PBPP can be used for developing anticancer products.

Description

technical field [0001] The invention relates to a preparation method of polysaccharides, in particular to a polysaccharide PBPP with anticancer activity of Phellinus chinensis fruiting bodies and a preparation method thereof. Background technique [0002] Polysaccharides are polymeric carbohydrates formed by the polymerization of more than 10 monosaccharides connected by glycosidic bonds, which are widely distributed in nature as structural components, storage nutrients, and special active ingredients. Due to its unique pharmacological effects and less toxic side effects, natural active polysaccharides show broad research and application prospects. [0003] Phellinus baumii (Phellinusbaumii) is a kind of fungal fruiting body with various pharmacological effects, which is named after parasitizing on mulberry trees. Traditional Chinese medicine believes that Phellinus can benefit the five internal organs, soften and harden, detoxify, stop bleeding, etc. Modern medical researc...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
CPCC08B37/0003
Inventor 时连根曾鹏
Owner ZHEJIANG UNIV
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