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Artificial cultivation method of Phellinus linteus

A technology of artificial cultivation and phellinus, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of poor fruiting body quality, low yield, immature technology, etc., achieve high yield, promote growth speed, good quality effect

Inactive Publication Date: 2011-06-15
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by this invention is to provide a new method for artificially cultivating Phellinus Phellinus at defects such as immature technology, low yield, and poor fruit body quality (not formed) in the existing Phellinus artificial cultivation method. Method, this method uses bags to cultivate Phellinus chinensis, explores the most suitable environmental factors such as air relative humidity, temperature, and light for the development of Phellinus chinensis fruiting bodies, and has the advantages of high yield, good fruiting body quality, and perfect technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Purify the bacterial strain isolated from the fruiting body of Phellinus baumii Pilát;

[0034] Isolate strains by tissue separation method: use a sterile scalpel to cut the middle part of the fruiting body of Phellinus phellinus longitudinally into two halves, and cut out a small square tissue of about 0.3 cm with a knife. Use tweezers to transfer the fungus tissue to the center of the test tube culture medium. Among them, the utensils and the cut tissue should never be in contact with other parts of Phellinus thallus to avoid contamination. After inoculation, place After culturing in a constant temperature darkroom at 25°C for 2 to 3 days, yellow fluffy hyphae can be seen growing from the tissue block. At this time, it must be observed continuously for a week to check the contamination of bacteria. If there are blue, green, yellow, orange and other small dots and paste, it means that the bacteria have been contaminated and should be removed in time. When the Phellinu...

Embodiment 2

[0039] The difference between this embodiment and Example 1 is: in the process of fruiting cultivation after Phellinus mycelium is covered with cultivation bags, the temperature in the fruiting chamber is 22°C, and the relative air humidity is always constant at 90%; A crescent-shaped mouth is drawn on the shoulder, the length of the mouth is 4cm, and the width of the mouth is 1cm; except that, all the other are the same as in Example 1.

[0040] Cultivation results of Phellinus fruiting bodies: It takes about 50 days for Phellinus sangha to grow into finished products. The color of Sanghuang fruiting bodies is bright yellow to yellowish brown, and most of them are semicircular. Each bag can produce dry Sanghuang fruiting bodies of up to 25g, wherein the fruit body is 5.9cm long, 4.6cm wide and 2.3cm thick. The internal organization of the fruiting body is tightly arranged, and the mass of the fruiting body per unit volume is relatively heavy.

Embodiment 3

[0042] The difference between this embodiment and embodiment 1 is: during the process of fruiting cultivation after Phellinus mycelium is overgrown with the cultivation bag, the temperature in the fruiting room is 24°C, and the relative air humidity is always constant at 87%; Stagger and draw 3 crescent-shaped mouths, the length of the mouth is 3cm, and the width of the mouth is 1cm; except that, the rest are the same as in Example 1.

[0043] Cultivation results of the fruiting bodies of Phellinus Phellinus: It takes about 50 days for Phellinus japonica to mature from incision to maturity. Due to the large number of fruiting openings, each fruiting body grown is small and irregular, and the quality is light. Each bag can produce mulberry The dry product of the yellow fruiting body reaches 21.6g, of which the length is 4.1cm, the width is 3.8cm, and the thickness is 2.1cm.

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Abstract

The invention discloses an artificial cultivation method for Phellinus baumii Pilat. The method comprises the following steps: (1) inoculating the purified strain separated from a Phellinus baumii Pilat fruiting body to a mother seed culture medium so as to obtain a mother seed; (2) inoculating the mother seed to an original seed culture medium so as to obtain a Phellinus baumii Pilat original seed; (3) inoculating the Phellinus baumii Pilat original seed to a cultivar culture medium inside a cultivation bag for culture; and (4) transplanting the cultivation bag fully covered by hypha into a mushroom producing room and carrying out the mushroom producing culture under the following conditions: 85 to 95 percent of relative air humidity and 22 to 25 DEG C of temperature. The method finds out the best environmental factors such as the relative air humidity, the temperature, the illumination, and the like for the growth of the Phellinus baumii Pilat fruiting body. Through the artificial accurate control on the factors of relative humidity and temperature in the mushroom producing room, the method can maximally improve the growth speed of the Phellinus baumii Pilat fruiting body while simultaneously ensuring the quality of the Phellinus baumii Pilat fruiting body. The method has the advantages of high yield, high Phellinus baumii Pilat fruiting body quality, material conservation, short production cycle, and the like.

Description

technical field [0001] The invention relates to a cultivation method of medicinal fungi, in particular to an artificial cultivation method of Phellinus baumii Pilát, belonging to the field of artificial cultivation of medicinal fungi. Background technique [0002] Phellinus baumii Pilát is also known as Sang Chen, Sang Er, Phellinus baumii and so on. Taxonomically, it belongs to Basidiomycota, Hymenomycetes, Aphyllophorales, Hymenochaetaceae, and Phellinus. It is a large and rare medicinal fungus . [0003] Phellinus is currently internationally recognized as the most effective medicinal fungus in the field of biological anti-cancer. In recent years, scholars at home and abroad have made further research on the pharmacological effects and chemical components of Phellinus, showing that Phellinus has anti-cancer, anti-tumor, and immunoregulatory effects (Shihata S, Nishikawa Y. Mai CF et al. Anti-tumor studies on some extracts of Basidiomycetes[J]. Gann, 1968, 59: 159~161; ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 杜萍戴玉成崔宝凯张春凤
Owner BEIJING FORESTRY UNIVERSITY
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