66 results about "Phellorinia" patented technology

The invention discloses a large-scale artificial cultivation method for phellinus igniarius and mainly solves the problem that an existing method can not cultivate sporocarps rapidly in a large-scale mode. The method comprises the following steps: 1, obtaining protospecies, and inoculating the protospecies into a cultivating bag of a phellinus igniarius large-scale artificial medium for cultivation; 2, when phellinus igniarius hyphae overgrow the cultivating bag and the hyphae are deep yellow, or protruding nodular phellinus igniarius primordium starts to appear in the cultivating bag, transferring the cultivating bag full of the hyphae into a fruiting house for conducting fruiting cultivation. The cultivation environment conditions inside the fruiting house is that the relative air humidity ranges from 85% to 98%, and the temperature ranges from 22 DEG C to 28 DEG C. The method has the advantages that the phellinus igniarius sporocarps can be cultivated rapidly in a large-scale mode.

The invention aims at providing a preparation method of a Phellinus linteus mycelium. The method comprises the following steps of: activating Phellinus linteus spawn; executing plate cultivation of potato dextrose agar culture medium; scraping the Phellinus linteus mycelium to inoculate the scraped Phellinus linteus mycelium to the potato dextrose agar culture medium for shake cultivation; collecting the Phellinus linteus mycelium; extracting Phellinus linteus mycelium total RNA; and observing result via agarose gel electrophoresis. The spawn used in the preparation method is the Phellinus linteus spawn (Phellinus baumii Pilate) collected in the CCTCC (China Center for Type Culture Collection), and the collection number is Phellinus linteus DL101 CCTCC M 2011137. The preparation method is simple to operate and has straightforward principle, and a simple and convenient method for obtaining large-scale high-purity Phellinus linteus mycelium and high-quality Phellinus linteus total RNA in the future is provided, and the basis for fundamental research and application development of the Phellinus linteus is also provided.

The invention discloses a phellinus igniarius strain and a culturing method for the same. The strain is classified and named as Inonotus sanghuang, and is preserved with a preservation number CGMCC No.6395. The culturing method for the strain comprises the following steps of 1, preparing a culture medium; 2, performing inoculation, namely extracting a phellinus igniarius strain block in n aseptic environment by virtue of an inoculation tool, transferring the phellinus igniarius strain block into the middle part of a test tube culture medium, and plugging the test tube culture medium with cotton seeds; 3, performing strain culturing, namely transferring a test tube inoculated in step 2 into a constant temperature incubator, and performing constant temperature culturing for 10 to 15 days at 25 to 35 DEG C in a darkroom. According to the phellinus igniarius strain and the culturing method for the same, the phellinus igniarius strain cultured by the method is discovered and separated from a phellinus igniarius strain SH21022 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp,nov) on mulberries, and grows vigorously and rapidly, and large-scale mycelium fermentation production, active ingredient extraction and healthcare product or medicament development can be realized to meet market requirements.

The invention discloses an extract for artificially cultured phellinus igniarius fruiting bodies as well as a preparation method and application for the extract. The preparation method comprises the following steps of selecting Sangdu No. 1 as a phellinus igniarius strain, adopting a bagging cultivation method in imitating a wild condition under a forest, harvesting the fruiting bodies when the color of the fruiting bodies changes from yellow to brown or dark brown, and obtaining the phellinus igniarius fruiting bodies; and crushing the phellinus igniarius fruiting bodies into powder, soakingthe powder with an ethanol aqueous solution, performing reflux extraction, performing vacuum concentration on an extracting solution until an ethanol smell does not exist, and performing vacuum dryingto obtain the extract for the artificially cultured phellinus igniarius fruiting bodies. The extract disclosed by the invention is capable of improving and relieving kidney injury of a mouse with hyperuricemia and increasing uric acid excretion, is moderate in effect and less in side effects and can be used for preparing the drug and the health-care product for preventing and treating the hyperuricemia and gout.

The invention discloses a cultivation material for the artificial cultivation of Phellinus igniarius, and a preparation method of the cultivation material. The preparation method comprises the following steps: (1) crushing mulberry twigs, cotton seed hulls and bran and then performing uniform mixing to obtain a mixture A; (2) performing steam explosion treatment on the mixture A to obtain a mixture B; (3) preparing a liquid culture medium with grapefruit peel hydrolysate as a raw material, performing inoculation with Acetobacter xylinum, and performing mechanical stirring and fermentation to obtain a fermentation broth C; (4) uniformly mixing the mixture B, the fermentation broth C, a vermiculite powder and quicklime, and performing microwave expansion treatment to obtain a mixture D; (5)fully dissolving sucrose and potassium dihydrogen phosphate in water to obtain a nutrient solution E; and (6) fully stirring the mixture D and the nutrient solution E uniformly, and then performing pressing to obtain the cultivation material. The cultivation material prepared by the invention not only can provide nutrient components required for the growth of Phellinus igniarius and improve the yield of Phellinus igniarius fruiting bodies, but also can improve the content of active components of Phellinus igniarius and improve product quality.

The invention relates to an efficient preparation method for for acquiring phellinus igniarius polyphenols from submerged fermentation mycelia of phellinus igniarius. The method comprises the following steps: inoculating a phellinus igniarius strain into a phellinus igniarius mycelium culture medium, carrying out submerged fermentation culture on the inoculated phellinus igniarius mycelium culture medium, freeze-drying the obtained phellinus igniarius mycelia, conducting pulverizing, and carrying out screening with a 40-mesh sieve to obtain phellinus igniarius mycelium powder; mixing the S phellinus igniarius mycelium powder with a deep eutectic solvent having a water content of 30%-50% according to a mass ratio of 1: 30 to 1: 50; extracting an S mixed solution in an ultrasonic extractor for 30-50 minutes, conducting centrifuging in an ultracentrifuge, and taking a supernatant; separating and purifying the supernatant through D101 macroporous resin to obtain a refined phellinus igniarius polyphenol mixed solution, and removing ethanol from the mixed solution through rotary evaporation to obtain refined phellinus igniarius polyphenols. According to the method, phellinus igniarius polyphenol can be more effectively extracted, the phellinus igniarius mycelia can be fully utilized, resources are not wasted, and the method is environmentally friendly.

The invention discloses a method for increasing the yield of intercellular polysaccharide in phellinus igniarius mycelium fermentation process by utilizing an ultrasonic technique and belongs to the technical field of food processing. According to the method, ultrasonic treatment is carried out in different stages (2-6 days) of mycelium fermentation; phellinus igniarius mycelium fermentation is assisted by ultrasonic water bath at the temperature of 26 DEG C; the frequency and power of the ultrasonic treatment are respectively set as 68kHz and 600W; the time of the ultrasonic treatment is 30-90 minutes; the ultrasonic intermittent ratio is 20s / 5s-30s / 5s. Proven by the measurement on the content of the mycelium intercellular polysaccharide, the yield of the intercellular polysaccharide in the phellinus igniarius mycelium fermentation process can be remarkably increased (2.51%-22.67%); the method is simple and efficient in process.

The invention discloses a recombinant vector used for phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body genetic expression, a construction method for the recombinant vector,and a recombinant vector genetic transformation method. The sequence of the vector is disclosed by SEQ ID No:13. The construction method comprises the following step of replacing a CaMV35S promoter of a binary vector pCAMBIA-1301 with a phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body homologous promoter to obtain a binary vector suitable for the phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body. The recombinant vector genetic transformation method comprises the following steps of: utilizing a freeze thawing method to convert the recombinant binary vector into agrobacterium tumefaciens, and obtaining positive bacteria; utilizing the agrobacterium tumefaciens to infect phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. hyphae, carrying out co-culture on a culture medium, and subsequently, covering a low melting point PDA culture medium; and then, transferring bacterial colonies to a new culture medium, extracting DNA from the bacterial colony which grows luxuriantly, and utilizing a PCR technology to identify a positive bacterial strain. The recombinant vector has the advantages of being simple in operation, high ina conversion rate and the like, the phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body genetic expression is successfully realized, homologous knockout, overexpression and heterologous expression in the phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body become possible, and the problem that a phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius(L.) Fr. fruiting body genetic transformation system can not be established is solved.

The invention discloses a liquid fermentation method for increasing the yield of phellinus igniarius mycelia. The liquid fermentation method comprises the following steps of S1, preparing a liquid culture medium for phellinus igniarius culture; S2, inoculating phellinus igniarius strain into the liquid culture medium, performing activation culture, inoculating into a seed culture medium accordingto the inoculation amount of 10-15% by volume, and performing seed culture to obtain a seed solution; and S3, inoculating the seed solution prepared in the step S2 into a liquid culture medium according to the inoculation amount of 10-15% by volume, performing liquid fermentation culture for 1-3 days at 28-35 DEG C, adding a certain amount of vegetable oil and magnesium acetate tetrahydrate in anyday in the culture process, continuously culturing for 2-3 days at 23-28 DEG C to obtain a mixed solution of the phellinus igniarius mycelium and fermentation liquor. The invention aims to provide the liquid fermentation method for improving the yield of the phellinus igniarius mycelium, and the yield is effectively improved.

The invention discloses a compound phellinus igniarius medicine for treating hand-foot-and-mouth disease and a preparation method thereof, and belongs to the technical field of medical health products. The preparation method comprises: inoculating a phellinus igniarius strain to a solid fermentation culture medium, performing fermentation to obtain phellinus igniarius mycelia, then performing leaching, rotary evaporation, centrifugation, re-dissolution, protein removal, pigment removal and dialysis to prepare phellinus igniarius polysaccharides from the phellinus igniarius mycelia, and then matching the phellinus igniarius polysaccharides with extracts of licorice root, weeping forsythia capsule and indigowoad root to prepare a compound medicine by mixing. The prepared compound phellinus igniarius polysaccharide medicine can effectively resist an enteric virus disease of type 71, and has a relatively good effect of treating the hand-foot-and-mouth disease.

The invention discloses a preparation method of a phellinus igniarius active ingredient, which comprises the following steps: taking a phellinus igniarius strain (Sanghuangporus sanghuang) fermentation liquor with the preservation number of CGMCC NO.21068, filtering mycelia, separating to obtain a phellinus igniarius fermentation liquor, heating and concentrating, adding 95% ethanol for soaking treatment, centrifuging, collecting supernate, recycling ethanol by using a rotary evaporator, collecting an alcohol-soluble substance, adding water for dissolving, freezing at -20 DEG C, performing vacuum freeze drying treatment, and crushing to obtain a phellinus igniarius fermentation liquor alcohol-soluble sample. The phellinus igniarius fermentation liquor alcohol-soluble sample is subjected to fractional extraction by using four organic solvents to obtain an extract. The research result provides a certain test basis and theoretical basis for subsequent development and utilization of phellinus igniarius liquid fermentation active substances and research and development of food, health care products or medicines related to blood sugar reduction and the like, and has certain theoretical significance and practical application value.

The invention belongs to the technical field of edible and medicinal fungus production, and particularly relates to a production method of a high-quality phellinus igniarius strain. The method comprises mother strain culture, stock culture and culture in cultivation. A mother strain culture medium is prepared from the following raw materials in parts by weight of 80-120 parts of mulberry sawdust,80-120 parts of potatoes, 15-25 parts of glucose, 8-12 parts of agar powder and 800-1200 parts of water; a stock culture medium and a cultivar culture medium are prepared from the following raw materials in percentage by weight of 72.5-82.5% of mulberry sawdust, 15-25% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; and the water content of the culture media is 60-70%. The phellinus linteus strain cultured by the method is stable in quality and high in growth speed; after the strain is inoculated into a mulberry sawdust cultivation material for about 45 days, primordia begin to form, the cultivation period is shortened, strain production is carried out on the mulberry sawdust, and a strain adaptive cultivation method is provided for mulberry planting; and the operation process is simple and easy to implement, convenient to control and suitable for large-scale cultivation of phellinus linteus strains.

The invention relates to a phellinus igniarius thallus, which capable of directly growing on the surface of a hydrophilic microporous membrane of a saturated adsorption medium to form a phellinus igniarius film; the phellinus igniarius film forms a biosensor based on immobilized phellinus igniarius thallus; the biosensor includes a gold electrode, a porous layer, the phellinus igniarius film, a microporous membrane, a rubber O-shaped ring, and an elastic electrode sleeve; the porous layer, the phellinus igniarius film and the microporous membrane are arranged on the one end surface of the goldelectrode from the inside to the outside; the elastic electrode sleeve is a hollow cylindrical structure; the elastic electrode sleeve is fixed on the outside of the gold electrode, and the outer edge of the microporous membrane covering the phellinus igniarius film is pressed on the surface of the gold electrode; the rubber O-shaped ring is embedded in the elastic electrode sleeve and disposed outside the microporous membrane, and the microporous membrane is fastened again. The biosensor based on immobilized phellinus igniarius thallus is used for detecting chlorophenol substances in wastewater so as to improve the anti-interference ability of the chlorophenol determination and reduce the detection cost.

The invention belongs to the technical field of artificial cultivation of edible fungi, and particularly relates to a method for efficiently cultivating phellinus igniarius sporocarp based on an artificial bag material. According to the method for efficiently cultivating the phellinus igniarius sporocarp based on the artificial bag material, the phellinus igniarius sporocarp is cultivated based ona traditional artificial bag material type cultivation mode, mulberry twig sawdust, wine brewing waste residues and molasses are selected as main cultivation raw materials, and the reutilization of industrial waste materials such as the wine brewing waste residues and the molasses is realized under the condition of meeting the nutritional ingredients of phellinus igniarius cultivation; and further added activated sludge particles depend on components such as microorganisms and organic matters contained in the activated sludge particles, and promote absorption of nutrient components of the cultivation raw materials and forming of phellinus igniarius mycelia and sporocarp greatly, so that the cultivation period of phellinus igniarius is greatly shortened, the cultivation efficiency of the phellinus igniarius sporocarp is effectively improved, and the yield and the quality guarantee are provided for the downstream application of the phellinus igniarius.

The invention discloses a culture method of phellinus baumii mycelia, a phellinus baumii beverage and an application of the phellinus baumii mycelia. The culture method comprises the following steps of (1) performing enlarged culture with a shake flask; (2) performing enlarged culture with a seed tank: inoculating fermentation culture media with shake flask second-class strains cultured and produced in the step (1), and performing enlarged culture so as to obtain first-class phellinus baumii strains; and (3) performing enlarged culture with a fermenter: inoculating the fermenter loaded with the fermentation culture media, with the first-class phellinus baumii strains obtained in the step (2), performing culture with the fermenter, and performing filtration so as to obtain the phellinus baumii mycelia. The phellinus baumii mycelia produced by the method are short in growth cycle and high in quality. Phellinus baumii mycelium powder is prepared through a freeze drying technique, and a beverage prepared based on the phellinus baumii mycelium powder has favorable health-care effects and has advantages of being uniform in beverage color, good in mouth feel, long in storage period and the like.

The invention discloses a phellinus baumii MADS-box transcription factor PbMADS1 as well as a coding gene and application thereof. The amino acid sequence of the transcription factor PbMADS1 is as shown in SEQ ID NO.3, and the sequence of the coding gene is as shown in SEQ ID NO.2. The invention further discloses application of the coding gene in regulation and control of the growth speed of phellinus baumii hyphae. Meanwhile, the invention also discloses an overexpression recombinant vector which comprises a transcription factor PbMADS1 coding gene sequence. Compared with a wild type (WT), the transgenic phellinus baumii strain (T) for expressing the PbMADS1 has the advantages that the mycelial growth speed can be increased by 17.5%, which indicates that the obtained transcription factor PbMADS1 participates in regulation and control of the growth and development of phellinus baumii mycelia, and the application of the transcription factor PbMADS1 in actual production is beneficial to shortening the artificial cultivation period of phellinus baumii.

The invention discloses a wild mulberry phellinus igniarius strain separation and purification method, and relates to the technical field of phellinus igniarius culture, phellinus igniarius has the advantages of high growth speed and short separation period, and the specific scheme is as follows: the method comprises the following steps: S1, performing surface disinfection on phellinus igniarius sporocarp under a sterile condition; S2, cutting off fungus blocks from the phellinus igniarius sporocarp and transplanting to a plate culture medium to be cultured under the dark condition; S3, observing the growth condition of phellinus igniarius, picking bright yellow or deep yellow hyphae through an inoculating loop when the bright yellow or deep yellow hyphae are found, separating on a new culture medium by adopting a four-region plate streaking method, and continuously culturing; S4, judging when infectious microbes appear, and repeating the step S3 when the infectious microbes affect the growth of the phellinus igniarius; when the infectious microbes do not affect the growth of the phellinus igniarius, the infectious microbes are removed under the sterile condition until purified phellinus igniarius is obtained; compared with a traditional broad-spectrum culture medium, the culture medium provided by the invention is not prone to infectious microbe infection and can resist infectious microbe.

The invention discloses a preparation for improving sleep by using phellinus igniarius. The preparation is prepared from the following components: 2000 to 3000g of phellinus igniarius extract, 3 to 5g of vitamin B6, 280 to 320g of lactose and 90 to 110g of magnesium stearate. The phellinus igniarius extract is prepared by the following method: taking phellinus igniarius, crushing, adding water, and extracting; repeatedly extracting for one time, combining the two leaching solutions, cooling to room temperature, concentrating under reduced pressure, adding 95% ethanol solution, standing, centrifugally separating and collecting precipitate; and redissolving the precipitate with water, and carrying out spray drying, thereby obtaining the product. According to the preparation for improving sleep by using the phellinus linteus, the phellinus linteus extract has the effects of resisting anxiety, calming and hypnotizing; vitamin B6 is a necessary substance for maintaining normal functions of a nervous system, and can more effectively pacify emotion. The preparation disclosed by the invention can effectively relieve mood and improve sleep for high-pressure people and people with poor sleep.

According to the liquid culture medium for phellinus igniarius mycelium culture and the culture method of the phellinus igniarius mycelium culture, the corn flour and the potato powder are used as main nutrient substances, and the mulberry sawdust extract and the liquorice extract are added, so that rapid growth of phellinus igniarius mycelium can be promoted; the medicinal activity of the artificially cultured phellinus igniarius mycelium is equivalent to that of wild phellinus igniarius; meanwhile, functional components such as nano-scale hydroxyapatite, triammonium citrate and magnesium acetate tetrahydrate are added, so that the activity of phellinus igniarius strains is kept, and the growth of phellinus igniarius mycelia is promoted. Meanwhile, parameters such as the pH value, the culture temperature, the culture rotating speed and the amount of introduced air in the phellinus igniarius mycelium culture process are optimized, and rapid growth of the phellinus igniarius mycelium can be promoted. The phellinus igniarius mycelium culture method provided by the invention is simple to operate, has the advantages of short growth cycle and high fermentation mycelium yield, and can be used for large-scale industrial production.

The invention discloses a rapid artificial cultivation method of mulberry phellinus, and relates to the technical field of phellinus cultivation. The problems that mulberry phellinus strains are degenerated, easily infected with mixed fungi and long in production cycle are solved, production efficiency is increased, time and economic cost are saved, and the problem about treatment of waste mulberry branches is solved to a certain degree; and additional value of mulberry is increased, income increase of silkworm raisers and cultivation persons is promoted, and one effective way is provided fortargeted poverty alleviation of the sericulture industry. According to the specific scheme, the method comprises following steps: greenhouse cultivation is performed, greenhouse planting parameters are as follows: humidity is controlled at 85%-95%; temperature is controlled at 25-30 DEG C; scattered light is adopted for irradiation, and transmissivity of light in the greenhouse is 5%-20%; the ventilation number every day is not less than 4 times; relative humidity in each fungus bag is kept at 55%-65%; and with adoption of a method for stimulating fruiting by log taping, phellinus bags are beaten every day. With adoption of the rapid artificial cultivation method of the mulberry phellinus, growth cycle of the mulberry phellinus is shortened from 6 months to 3 months, production efficiencyof the mulberry phellinus is improved, and economic costs such as time, manpower, material resources and the like are saved.

The invention discloses a production method of a phellinus igniarius mycelia product containing rich bioactive substances. The production method comprises the following steps: inoculating phellinus igniarius mycelia in a culture medium using mulberry stalks as raw materials, inducing the mycelia to change color in combination with the temperature and the illumination time, omitting a fruiting body formation stage, and culturing the mature mycelia product containing rich effective substances of polysaccharides, flavones, triterpenes, beta-sitosterol and alkaloids by a dry or wet separation process. The phellinus igniarius mycelia product cultured by the method is a green organic product with a healthcare function, has the effects of benefiting the five internal organs, softening hard mass, removing toxic materials, stopping bleeding, activating blood, harmonizing the stomach and relieving diarrhea, and is used for mainly treating gonorrhea, metrorrhagia, metrostaxis, morbid leucorrhea, abdominal mass accumulation, hypochondrium fluid retention and splenasthenic diarrhea; the effects of the phellinus igniarius mycelia product are not poorer than those of cordyceps sinensis.

The invention relates to a spore production device simulating wild environment and the application thereof to antrodia camphorata. The device comprises a spore production tank, a wood block laying basket, a sterile air diffusion plate and a spore collection plate, wherein a sealing cover, a temperature control system access port and a humidifying system access port are formed in the upper end of the spore production tank; the wood block laying basket is arranged in the spore production tank; the sterile air diffusion plate and the spore collection plate are respectively arranged on the two sides of the wood block laying basket; a nutrient solution instilling opening, a sterile air system inlet and a spore outlet are formed in the spore production tank. The spore production device can accelerate the reproduction of original spore, and can greatly shorten the spore formation time and prevent microbial contamination, meanwhile is good in activity of spore obtained through the device, andcan be applied to the reproduction of various fungi (such as antrodia camphorata, cordyceps sinensis and phellinus igniarius) spore, shorten the culture time and improve the biological activity.

The invention relates to a method for cultivating microbial strains of phellinus linteus, which is characterized in that the culture media of microbial strains comprises the following components by weight percent: 75-85% of sawdust of poplar or willow or mulberry, 10-15% of bran, 5-8% of rice bran, 1-2% of plaster, 0.2-0.5% of monopotassium phosphate, 0.1-0.3% of magnesium sulfate, wherein, the sum of the weight percentage of the components is 100%. The microbial strains of phellinus linteus prepared in the invention has rapid growing of microbial strains of phellinus linteus, not easy ageingand yellow water occurred on surface hypha; and the strains can expand completely after 30-35 day cultivation. By adopting the method for cultivating a phellinus linteus forest land, forestry land resources can be fully utilized to plant phellinus linteus products; the method is simple and practicable; and the produced phellinus linteus with large volume can be artificially cultivated in great amounts, thus fully meeting the market demand.

The invention discloses a preparation method of a phellinus igniarius extract for preventing cancer, resisting cancer and reducing blood pressure, which comprises the following steps: S10, providing a phellinus igniarius strain, drying at 45-65 DEG C, and grinding to form phellinus igniarius powder with a predetermined particle size; s20, mixing the phellinus igniarius powder and a 62% ethanol solution according to a weight ratio of (1-4): (30-43), and extracting under preset conditions to obtain a phellinus igniarius extracting solution; s30, centrifuging the phellinus igniarius extracting solution, collecting supernate, and cooling; and S40, performing vacuum drying after cooling to obtain the phellinus igniarius extract. Therefore, the corresponding extraction rates of the phellinus igniarius extract under different conditions can be accurately determined, and then reasonable extraction can be carried out under the best conditions.

The application discloses a production method of high-activity micromolecule composite edible ganoderma lucidum powder. The production method comprises the following step of uniformly mixing the following components in mixing percentage by mass of 85-60% of fomes japonica mycelium powder with 15-40% of phellinus igniarius mycelium powder to obtain the high-activity micromolecule composite edible ganoderma lucidum powder, wherein the water content of the high-activity micromolecule composite edible ganoderma lucidum powder is 5-7%. According to the production method disclosed by the invention,through selecting raw material kinds of culture mediums, adjusting proportion and applying a solid fermentation technique, the cost can be reduced, and high-activity fomes japonica mycelia and high-activity phellinus igniarius mycelia are effectively fermented and produced; and through low temperature drying, the effect for maintaining effective components is better. In addition, the invention further tests and researches fermentation conditions of different strains so as to optimize culture conditions.

The invention provides a mulberry phellinus igniarius strain and a cultivation method thereof, and belongs to the technical field of biology. The mulberry phellinus igniarius strain is specifically mulberry phellinus igniarius QJF-2, and the preservation number is CGMCC No.19653. The mulberry phellinus igniarius QJF-2 strain is high in medicinal value and is authentic mulberry phellinus igniarius, and the medicinal effect is obviously higher than that of common poplar phellinus igniarius.

The invention relates to a compound feed additive and a feed for laying hens in growing period. A preparation method of the compound feed additive comprises the following steps: mixing shrimp shell powder, bran and glucose, adjusting the water content, and sterilizing to obtain a mixture; inoculating phellinus igniarius hyphae into the mixture, and performing solid-state fermentation to obtain a fermented material; and drying and crushing the fermented material to obtain the feed. The invention also discloses the feed containing the compound feed additive for laying hens in a growing period. The compound feed additive disclosed by the invention is added into the feed for laying hens in a growing period, so that the yield and the quality of eggs can be remarkably improved, and the requirements of the market on high-quality eggs can be well met.