65 results about "Phellorinia" patented technology

The invention discloses a recombinant vector used for phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body genetic expression, a construction method for the recombinant vector,and a recombinant vector genetic transformation method. The sequence of the vector is disclosed by SEQ ID No:13. The construction method comprises the following step of replacing a CaMV35S promoter of a binary vector pCAMBIA-1301 with a phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body homologous promoter to obtain a binary vector suitable for the phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body. The recombinant vector genetic transformation method comprises the following steps of: utilizing a freeze thawing method to convert the recombinant binary vector into agrobacterium tumefaciens, and obtaining positive bacteria; utilizing the agrobacterium tumefaciens to infect phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. hyphae, carrying out co-culture on a culture medium, and subsequently, covering a low melting point PDA culture medium; and then, transferring bacterial colonies to a new culture medium, extracting DNA from the bacterial colony which grows luxuriantly, and utilizing a PCR technology to identify a positive bacterial strain. The recombinant vector has the advantages of being simple in operation, high ina conversion rate and the like, the phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body genetic expression is successfully realized, homologous knockout, overexpression and heterologous expression in the phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius (L.) Fr. fruiting body become possible, and the problem that a phellinus igniarius (L.ex Fr.) Quel. [Fomes igniarius(L.) Fr. fruiting body genetic transformation system can not be established is solved.

The present invention relates to the field of biotechnology, and specifically relates to an original ecological imitation wild cultivation method of Phellinus phellidis. (2) The original Phellinus seed was inoculated on the original seed medium to obtain the original Phellinus seed; (3) The original Phellinus seed was inoculated into the wooden nail medium in the cultivation bag to obtain the (4) The wooden nail cultivar covered with Phellinus mycelium is inoculated on the living body of mulberry tree for natural cultivation. The present invention obtains Inonotus by separating and purifying wild Phellinus? sanghuang ( Phellinus ) strains, and then directly inoculate the strains on the live mulberry trees in the mulberry garden, and use the live mulberry trees as the growth host of Phellinus sanghuang to artificially increase the probability of the mulberry trees being infected with Phellinus sphaerifolia. Wild similar, higher medicinal value.

The invention discloses an extract of artificially cultivated Phellinus phellicis fruiting bodies and its preparation method and application. The preparation method comprises: selecting the Phellinus phellinus strain Sangdu No. 1, adopting a cultivation method imitating wild bag materials under natural forests, and making the fruiting bodies Harvest when the color turns from yellow to brown or dark brown, and then obtain the fruiting body of Phellinus japonica; grind the fruiting body of Phellinus japonica to obtain powder, soak in ethanol aqueous solution, reflux extraction, concentrate the extract under reduced pressure until it has no ethanol smell, vacuum Dry to obtain the extract of the artificially cultivated Phellinus radix fruiting bodies. The extract of the invention can improve and alleviate the kidney damage of mice with hyperuricemia, increase the excretion of uric acid, and has mild action and few side effects, and can be used to prepare oral medicine and health products for preventing and treating hyperuricemia and gout.

The invention discloses a production method of a phellinus igniarius mycelia product containing rich bioactive substances. The production method comprises the following steps: inoculating phellinus igniarius mycelia in a culture medium using mulberry stalks as raw materials, inducing the mycelia to change color in combination with the temperature and the illumination time, omitting a fruiting body formation stage, and culturing the mature mycelia product containing rich effective substances of polysaccharides, flavones, triterpenes, beta-sitosterol and alkaloids by a dry or wet separation process. The phellinus igniarius mycelia product cultured by the method is a green organic product with a healthcare function, has the effects of benefiting the five internal organs, softening hard mass, removing toxic materials, stopping bleeding, activating blood, harmonizing the stomach and relieving diarrhea, and is used for mainly treating gonorrhea, metrorrhagia, metrostaxis, morbid leucorrhea, abdominal mass accumulation, hypochondrium fluid retention and splenasthenic diarrhea; the effects of the phellinus igniarius mycelia product are not poorer than those of cordyceps sinensis.

The invention relates to a method for cultivating microbial strains of phellinus linteus, which is characterized in that the culture media of microbial strains comprises the following components by weight percent: 75-85% of sawdust of poplar or willow or mulberry, 10-15% of bran, 5-8% of rice bran, 1-2% of plaster, 0.2-0.5% of monopotassium phosphate, 0.1-0.3% of magnesium sulfate, wherein, the sum of the weight percentage of the components is 100%. The microbial strains of phellinus linteus prepared in the invention has rapid growing of microbial strains of phellinus linteus, not easy ageingand yellow water occurred on surface hypha; and the strains can expand completely after 30-35 day cultivation. By adopting the method for cultivating a phellinus linteus forest land, forestry land resources can be fully utilized to plant phellinus linteus products; the method is simple and practicable; and the produced phellinus linteus with large volume can be artificially cultivated in great amounts, thus fully meeting the market demand.

The invention provides a Phellinus strain of high yield polysaccharides, the strain number is S01, the preservation number is CGMCC NO.20223, and a cultivation method is provided: the Phellinus strain S01 of poplar is successively cultivated by mother species, original species, cultivated species and The Phellinus Phellinus fruiting bodies are obtained by culturing at the yellowing stage, and the application is also provided for preparing anti-tumor health food or medicine. The poplar phellinus strain S01 of the invention is genetically stable, and the polysaccharide content in the phellinus fruiting body obtained by the strain can reach 4.36%-4.59%. It has strong ability in synthesizing polysaccharides and can be used in the preparation of anti-tumor health food or medicine.