24 results about "Phellorinia" patented technology

The invention relates to a phellinus igniarius mycelium fermentation product and a preparation method and application thereof, and the preparation method comprises the following steps: inoculating a phellinus igniarius mycelium seed solution into a sterile fermentation culture medium for culturing, carrying out solid-liquid separation, filtering a clear liquid through a membrane and sterilizing to obtain the phellinus igniarius mycelium fermentation product, the fermentation medium comprises the following components: a beet extract, a deep eutectic solvent and water. The beet extract and the deep-eutectic solvent are creatively adopted as culture medium components to prepare the phellinus igniarius mycelium fermentation product, the beet extract and the deep-eutectic solvent jointly provide nutrients needed by phellinus igniarius fermentation, meanwhile, the deep-eutectic solvent is used for regulating metabolism, and compared with a product obtained through fermentation of a traditional culture medium, the phellinus igniarius mycelium fermentation product has the advantages that the fermentation efficiency is high; the content of active ingredients in a fermentation product obtained by adopting the culture medium with the formula is remarkably increased, and the fermentation product is good in uniformity and high in stability, is a clear liquid in appearance, has no abnormal odor and has slight phellinus igniarius characteristic odor.

The invention belongs to the technical field of fungus cultivation, and particularly relates to a cultivation method of high-yield and high-quality phellinus igniarius. The cultivation method comprises the following steps: (1) inoculating: preparing a culture medium, putting the culture medium into a plastic bag, sterilizing, cooling, and inoculating by using a phellinus igniarius strain; (2) spawn running management: culturing the inoculated plastic bag in a dark place; after 30-35 days of spawn running management, longitudinally extruding and shortening the fungus bags, continuing to culture, and entering fruiting management; (3) fruiting management: transferring the maturely cultured phellinus igniarius fungus bags into a fruiting greenhouse, marking rectangular fruiting openings in the middle parts of the fungus bags, and carrying out phellinus igniarius fruiting management; and (4) harvesting: obtaining the phellinus igniarius sporocarp 100-120 days after cutting. According to the cultivation method for the high-yield and high-quality phellinus igniarius, the biotransformation efficiency and the content of effective components of the phellinus igniarius are remarkably improved by optimizing the components and the proportion of the culture medium, and the technical problems that in traditional cultivation, the yield is low, and the content of the effective components is low are solved.

The application discloses a kind of based on fermentation of Sanghuangjun preparation's Radix Tetrastigae polysaccharide and its use.The Radix Tetrastigae polysaccharide is composed of polysaccharide with weight percentage content more than 99%;The polysaccharide is composed of galactose, mannose, fucose and 3-O-methyl-galactose, and the molar ratio is 8.5-11.0:1.5-3:0.8-1.5:1.0.The Radix Tetrastigae polysaccharide is extracted, separated and purified from Sanghuang fermentation Radix Tetrastigae.The Radix Tetrastigae polysaccharide has significant antitumor activity and regulates intestinal flora structure efficacy, can be directly used as antitumor and / or regulate intestinal flora structure function product, and also can be used for preparing antitumor and / or regulating intestinal flora structure function product.

The invention relates to phellinus igniarius fermented phaseolus calcaratus nutrition powder and a preparation method thereof. The preparation method comprises the following steps: soaking phaseolus calcaratus in water, sterilizing, inoculating a phellinus igniarius mother strain, and culturing to obtain a phellinus igniarius stock strain; soaking the phaseolus calcaratus in water, sterilizing, inoculating a phellinus igniarius stock seed, and performing primary fermentation culture to obtain a primary culture; crushing the primary culture into blocks and carrying out secondary fermentation culture to obtain a secondary culture; and drying and crushing the secondary culture to obtain the phellinus igniarius fermented phaseolus calcaratus nutrition powder. The invention also provides the nutrition powder prepared by the preparation method and application of the phellinus igniarius in preparation of fermented phaseolus calcaratus. The phellinus igniarius fermented phaseolus calcaratus nutrition powder prepared by the method disclosed by the invention has medicinal components of phellinus igniarius mycelia and nutritional components of grain phaseolus calcaratus, has high nutritional value and health-care efficacy, and can also be used for fermenting other grains by using phellinus igniarius according to the method disclosed by the invention; or other medicinal fungi are used for fermenting different grains.

The invention discloses a rapid phellinus igniarius fungus stick decomposition method suitable for a bamboo forest environment. The rapid phellinus igniarius fungus stick decomposition method comprises the following steps: mechanically crushing fungus sticks; a decomposition flora is introduced; a bamboo forest ecological circulation system is constructed; the mushroom stick substrate comprises the following components: 30-40% of bamboo sawdust; 20%-30% of mulberry twig sawdust; 15%-20% of wheat bran; 10%-15% of corncob; 1%-2% of gypsum; 0.5%-1% of a phosphate fertilizer; the content of cellulase is 0.1%-0.5%, the content of hemicellulase is 0.05%-0.1%, and the content of ligninase is 0.05% The phellinus igniarius fungus stick has the advantage that the phellinus igniarius fungus stick can be quickly decomposed in the bamboo forest land.

The present application relates to a kind of phellinus igniarius mycelium fermentation product and its preparation method and application, the preparation method includes the following steps: phellinus igniarius mycelium seed liquid is inoculated in sterile fermentation medium and is cultured, solid-liquid separation, clear liquid is filtered and sterilized, and the phellinus igniarius mycelium fermentation product is obtained;The component of the fermentation medium includes beet extract, eutectic solvent and water.The present application creatively uses beet extract and eutectic solvent as medium component to prepare phellinus igniarius mycelium fermentation product, wherein beet extract and eutectic solvent provide the nutrition required for phellinus fermentation together, while using eutectic solvent to regulate metabolism, compared with the product obtained by traditional medium fermentation, the active ingredient content in the fermentation product obtained by the medium with the above formula is significantly improved, and the uniformity of fermentation product is good and stability is high, appearance is clear liquid, without abnormal odor, with slight phellinus characteristic odor.

The invention relates to a phellinus igniarius facility cultivation system and method.The phellinus igniarius facility cultivation system comprises an outer-layer large arched shed, a plurality of inner-layer small arched sheds, an environment monitoring module and an environment regulation and control module, the multiple inner-layer small arched sheds are arranged in the outer-layer large arched shed, and the inner-layer small arched sheds are used for containing phellinus igniarius fungus bags; the environment monitoring module is used for monitoring environment parameters of the inner-layer small arched shed; and the environment regulation and control module is used for regulating and controlling the environment parameters in response to the monitoring result of the environment monitoring module. According to the technical scheme, the outer-layer large arched shed and the inner-layer small arched shed are arranged, the environment monitoring module is combined to monitor the environment parameters in real time, and the environment regulation and control module automatically responds to regulation and control, so that precise management of the environment parameters is achieved, real-time monitoring and automatic regulation and control of the environment parameters can be achieved, the manual intervention requirement is reduced, and the working efficiency is improved. The production efficiency and the phellinus igniarius quality are improved, and the cost benefit is considered.

The invention provides phellinus igniarius-Chinese yam fermentation mycoplasm as well as a preparation method and application thereof, and relates to the technical field of microbial fermentation. Through a bidirectional fermentation technology, the Chinese yam is used as a fermentation culture medium, the black beans are used as a supplementary nitrogen source, and the phellinus igniarius strain is used as a fermentation strain for bidirectional fermentation. On one hand, by adopting the fermentation method, the growth speed of phellinus igniarius mycelia and the synthesis efficiency of active ingredients are remarkably improved, and the content of various active ingredients such as flavone, polyphenol and polysaccharide in a fermentation product is remarkably improved. On the other hand, the fermented mycoplasm obtained by the method is also rich in various active ingredients such as flavone, polyphenol, polysaccharide, alkaloid and the like, and has remarkable oxidation resistance.

The application discloses a selenium-rich Phellinus baumii strain domestication, cultivation and selenium enrichment method, which follows the gradient domestication principle of low-selenium starting and gradual increase, sequentially completes selenium source domestication of the Phellinus baumii primary mother strain, secondary original strain and tertiary cultivation strain, and obtains the cultivation strain suitable for the high-selenium environment; then, the tertiary cultivation strain is taken as the inoculation source, standardized cultivation is carried out by adopting the bagged solid culture medium, and the temperature, humidity, light and air conditions are regulated and controlled in two stages of mycelium culture and fruiting body growth management; and through gradient selenium source supply, continuous selenium supplement during the cultivation period, accurate regulation and control of the environmental conditions and scientific treatment after harvesting, efficient absorption and conversion of selenium by the Phellinus baumii are realized, inorganic selenium is converted into organic selenium, and the organic selenium is enriched in the fruiting body. The application solves the problems of low selenium tolerance and poor selenium enrichment efficiency of the Phellinus baumii, the selenium-rich Phellinus baumii fruiting body has high and stable organic selenium content, and the strain domestication success rate and cultivation survival rate are greatly improved.

The present application relates to the technical field of edible and medicinal fungus fermentation, and discloses a fermentation method of Phellinus baumii with high Hispidin yield and application.The fermentation method of Phellinus baumii in the present application comprises inoculating Phellinus baumii strains into a culture medium containing copper ion compounds, and then fermenting to obtain the product.The mycelium of Phellinus baumii obtained by the fermentation method of the present application is rich in Hispidin, and can be widely used in the preparation of related drugs and health foods for liver protection, anti-tumor, anti-virus, oxidation resistance, uric acid reduction, etc.In addition, the present application also discloses a high-performance liquid chromatography detection method for analyzing the content of Hispidin, which can effectively analyze the content of Hispidin and its precursor substances (such as protocatechuic acid, protocatechuic aldehyde, coffee acid, and aspidin) in the fermentation product of Phellinus baumii, its derivative product, and the mycelium of Phellinus baumii, and is helpful to improve the accuracy of Hispidin detection.

The invention belongs to the field of bioengineering, and particularly relates to a wild phellinus igniarius SH-27 sporocarp polyphenol extract and application thereof. The wild phellinus igniarius SH-27 sporocarp polyphenol extract provided by the invention is obtained by extracting wild phellinus igniarius SH-27 sporocarp preserved in Guangdong Microbiological Culture Collection Center, the wild phellinus igniarius SH-27 is Inonotus hispidus, and the preservation number is GDMCC No: 65865. The wild phellinus igniarius SH-27 used in the invention is a rare medicinal and edible fungus and contains rich active ingredients, the polyphenol content of mycelium pellets cultured by liquid of the wild phellinus igniarius SH-27 is 29.74 mg / g, the triterpene content is 3.52 mg / g, the polysaccharide content is 88.61 mg / g and the flavone content is 5.14 mg / g, the polyphenol content of the mycelium pellets can reach 46.98 mg / g by optimizing a liquid culture medium, the polyphenol content of sporocarps cultured by the wild phellinus igniarius SH-27 can reach 77.32 mg / g, and the content of the sporocarps cultured by the wild phellinus igniarius SH-27 can reach 77.32 mg / g. The sporocarp polyphenol extract has huge medicinal potential in the aspects of treatment and intervention of oxidation resistance, inflammation resistance, cancer resistance, tumor resistance, diabetes and hypertension and the like.

The invention relates to phellinus igniarius mulberry leaf tea and a preparation method thereof.The method comprises the following steps that 1, mulberry leaf chippings are blended into a wet material with water, the wet material is inoculated with phellinus igniarius strains for fermentation culture, and phellinus igniarius stock seeds are obtained; (2) blending a mixture containing mulberry leaf debris and a starch material into a culture material with water, and inoculating the phellinus igniarius stock seed for fermentation culture to obtain a first culture material; (3) crushing the first culture material into blocks, and continuously culturing to obtain a second culture material; and (4) pressing and drying the second culture material to obtain the phellinus igniarius and mulberry leaf tea. The invention also provides the phellinus igniarius and mulberry leaf tea prepared according to the method, a novel inonotus hirsutus strain Chengyuan 183-1 and application of the inonotus hirsutus strain Chengyuan 183-1 in preparation of the phellinus igniarius and mulberry leaf tea. The phellinus igniarius and mulberry leaf tea prepared by the method has nutritional and medicinal components of the phellinus igniarius mycelia and the mulberry leaves, and has high nutritional value and health-care efficacy.

The utility model discloses a phellinus igniarius strain culture device, which belongs to the technical field of phellinus igniarius strain culture, and comprises a bottom plate, a placing disc and a motor, the top surface of the bottom plate is communicated with a culture cylinder, the placing disc is rotatably arranged on the inner top surface of the culture cylinder, the top surface of the bottom plate is provided with a circular groove, and the motor is arranged in the circular groove. A hollow disc is rotatably mounted in the circular groove, the top surface of the hollow disc is communicated with two vertical pipes used for heating phellinus igniarius strains, the side walls of the vertical pipes are communicated with a plurality of air outlet pipes used for outputting hot air, the tail end of a rotating shaft of the motor is fixedly connected with a rotating pipe, the rotating pipe is communicated with the hollow disc, and the rotating pipe is fixedly connected with a rotating shaft of the motor. The side wall of the rotating pipe is rotationally sleeved with a sleeve. Compared with the prior art, the device can effectively and uniformly spray hot air to the surface of the phellinus igniarius strain culture dish placed on the top surface of the placing disc, so that phellinus igniarius strains can be uniformly heated.

The application discloses Sanghuangporus sanghuang, which is obtained by separating Sanghuangporus sanghuang fruiting bodies of authentic perennial wild mulberry trees, is named Sanghuangporus sanghuang CKS-327, has a preservation number of CCTCC NO: M 20242849, and is preserved in the China Center for Type Culture Collection on December 18, 2024, and the preservation address is Wuhan University, Biaoyi Road, Wuchang District, Wuhan City, Hubei Province. A method for cultivating Sanghuangporus sanghuang in an internal circulation heat and humidity exchange system, specifically comprising the following steps: (1) continuously stressing and stimulating Sanghuangporus sanghuang mother species domestication; (2) stressing and stimulating Sanghuangporus sanghuang fungus solid-liquid mixed fermentation agent culture; (3) cultivating and culturing fresh mulberry branch segments; and (4) managing yellow production in the internal circulation heat and humidity exchange system. The application ensures that the effective active ingredients of the wild perennial Sanghuangporus sanghuang are closest, improves the medicinal value of Sanghuangporus sanghuang, and maximally and continuously transforms and utilizes mulberry branch resources and water resources to produce Sanghuangporus sanghuang, and solves the problems of great management difficulty and low transformation efficiency of high-content mulberry branch sawdust bag cultivation and production of Sanghuangporus sanghuang.

The invention discloses a selenium source regulation and control method for directionally producing high-value selenium-rich phellinus igniarius mycelium or sporocarp, and belongs to the technical field of edible and medicinal fungus cultivation and microbial fermentation. The invention provides a target-oriented differential selenium source strategy aiming at the technical contradiction that a single selenium source cannot meet the requirements of different growth stages of phellinus igniarius in the prior art. When the phellinus igniarius mycelium is used as a target product, a fermentation strategy of adding selenium-enriched yeast in the whole process is adopted; when phellinus igniarius sporocarp is used as a target product, a staged strategy is adopted: selenium-enriched yeast is added in a mycelium culture stage, and sodium selenite is added in a sporocarp culture stage. According to the method, the rule that the mycelium stage is adaptive to organic selenium and the sporocarp stage is adaptive to inorganic selenium is defined for the first time, and through accurate stage-selenium source matching, the selenium content, yield and comprehensive quality of the mycelium or the sporocarp are synchronously improved successfully on the premise that mycelium growth is hardly inhibited.

The invention belongs to the technical field of biological fermentation, and particularly discloses phellinus igniarius mycelium and a preparation method and application thereof. The preparation method of the phellinus igniarius mycelium comprises the following steps: (1) strain activation: inoculating phellinus igniarius mycelium blocks into a PDA culture medium for culturing to obtain activated phellinus igniarius; (2) seed culture: inoculating the activated phellinus igniarius into a seed culture medium for culture to obtain a phellinus igniarius seed solution; (3) fermentation culture: transferring the phellinus igniarius seed solution into a fermentation culture medium for culture to obtain phellinus igniarius fermentation liquor; and (4) filtering and drying: filtering the phellinus igniarius fermentation liquor to obtain filter residues, cleaning the obtained filter residues, and drying to obtain the phellinus igniarius mycelium. The phellinus igniarius mycelium prepared by the method is high in yield and high in effective component content, and can be used as a raw material to be applied to health care products, cosmetics or medicines.

The invention provides a phellinus igniarius strain rich in polysaccharide and general flavone as well as a cultivation method and application thereof, relates to the technical field of biology, and aims to solve the problems that a phellinus igniarius variety which is high in yield, high in fruiting environment adaptability and high in polysaccharide and general flavone content is lacked in the prior art, and an existing cultivation method is difficult to meet the effects of high yield and income increase. The phellinus linteus strain is phellinus linteus spp, is named as Harmulberry No.1, and is preserved in the China General Microbiological Culture Collection Center on October 31, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41588. In the cultivation process of the phellinus igniarius sporocarp, a cultivar culture medium is prepared from, by mass, 78% of oak sawdust, 15% of trametes robiniophila cultivation waste, 5% of corn flour, 1% of gypsum powder and 1% of cane sugar. The phellinus igniarius variety disclosed by the invention has potential development and application values and can be used for preparing health-care foods or medicines.

The application belongs to the technical field of biological fermentation, and particularly discloses a phellinus igniarius mycelium, a preparation method and application thereof. The preparation method comprises the following steps: (1) activating the strain: inoculating phellinus igniarius mycelium pieces into PDA culture medium for culture to obtain activated phellinus igniarius; (2) seed culture: inoculating the activated phellinus igniarius into seed culture medium for culture to obtain phellinus igniarius seed liquid; (3) fermentation culture: transferring the phellinus igniarius seed liquid into fermentation culture medium for culture to obtain phellinus igniarius fermentation liquid; and (4) filtration and drying: filtering the phellinus igniarius fermentation liquid to obtain filter residue, washing and drying the filter residue to obtain the phellinus igniarius mycelium. The phellinus igniarius mycelium prepared by the method has high yield and high content of effective components, and can be used as raw material in health products, cosmetics or medicines.

The invention provides a culture method for rapidly increasing the content of polysaccharide in phellinus igniarius mycelium cells, and belongs to the technical field of phellinus igniarius culture.The culture method comprises the steps that mulberry leaves are cleaned, subjected to fixation and microwave drying and then crushed into 200-mesh mulberry leaf powder, then the mulberry leaf powder, cane sugar, corn starch and other components are prepared into a specific liquid culture medium, and the liquid culture medium is prepared into the phellinus igniarius mycelium cells. The phellinus igniarius mycelium grows rapidly through liquid culture, and the content of polysaccharide active ingredients in phellinus igniarius mycelium cells is remarkably increased. The phellinus igniarius thallus liquid culture method is rapid and efficient, the content of intracellular polysaccharide in the cultured phellinus igniarius mycelia is high, extraction is convenient and rapid, and the phellinus igniarius thallus liquid culture method can be suitable for large-scale industrial production.

The invention belongs to the technical field of biological fermentation, and particularly discloses a phellinus igniarius extract with whitening and oil control effects as well as a preparation method and application of the phellinus igniarius extract. The preparation method of the phellinus igniarius extract comprises the following steps: (1) strain activation: inoculating phellinus igniarius hypha blocks into a PDA culture medium for culturing to obtain activated phellinus igniarius; (2) seed culture: inoculating the activated phellinus igniarius into a seed culture medium for culture to obtain a phellinus igniarius seed solution; (3) fermentation culture: transferring the phellinus igniarius seed solution into a fermentation culture medium for culture to obtain phellinus igniarius fermentation liquor; and (4) extracting, namely treating the phellinus igniarius fermentation liquor by adopting ultrasonic waves, adding yeast powder and a mulberry extract, sufficiently and uniformly mixing, performing secondary fermentation treatment, and performing ultrasonic treatment, extraction, filtration, concentration and drying to obtain the phellinus igniarius extract. The prepared phellinus igniarius extract is high in active ingredient content and high in activity, and can be used as a raw material to be applied to health care products or cosmetics.

The invention discloses a method for increasing the content of phellinus igniarius polysaccharide in mycelium by adding an accelerant in a liquid fermentation process, which comprises the following steps of: putting a sterilized liquid culture medium into a culture container, inoculating by adopting a phellinus igniarius seed solution, covering a plug, and fermenting on a shaking table with the rotating speed of 120-180 rpm for 7-9 days under the conditions of constant temperature of 26-28 DEG C and light shielding; and after the phellinus igniarius mycelium in the liquid culture medium is proliferated for 18-36 hours, adding an accelerant into the liquid culture medium to induce the mycelium to generate more phellinus igniarius polysaccharides. According to the method for increasing the mycelium phellinus igniarius polysaccharide content by adding the accelerant in the liquid fermentation process, an accelerant carrier separated from a culture solution is arranged at the bottom of a bottle opening or a bottle plug of a culture container, and the culture container is inclined in the early and middle stages of mycelium development, so that a liquid culture medium is in contact with the accelerant and the accelerant is dissolved; the generation of polysaccharide is stimulated.

The invention discloses mulberry phellinus sanghuang, which is obtained by separating authentic perennial wild mulberry phellinus sporocarp, is named mulberry phellinus sanghuang CKS-327, has a preservation number of CCTCC NO: M 20242849, is preserved in China Center for Type Culture Collection on December 18, 2024, and is preserved in Wuhan University on eight road, Wuchang District, Wuhan City, Hubei Province. The invention relates to a method for cultivating phellinus igniarius by an internal circulation heat and humidity exchange system. The method specifically comprises the following steps: (1) continuous stress stimulation domestication of phellinus igniarius mother strains; (2) carrying out stress stimulation culture on the phellinus igniarius solid-liquid mixed leavening agent; (3) culturing fresh mulberry branch section cultispecies; and (4) performing yellowing management in the internal circulation heat and humidity exchange system. According to the method, the effective active ingredients closest to those of the phellinus igniarius living for many years are ensured, the medicinal value of the phellinus igniarius is improved, meanwhile, mulberry twig resources and water resources are continuously converted and utilized to the maximum extent to produce the mulberry phellinus igniarius, and the problems that the management difficulty is large, and the conversion efficiency is low when the mulberry phellinus igniarius is cultivated and produced through high-content mulberry twig sawdust bag materials are solved.

The present application belongs to the technical field of fungus cultivation, and particularly relates to a cultivation method of high-yield and high-quality Phellinus baumii. The cultivation method comprises the following steps: (1) inoculation: preparing a culture medium, filling the culture medium into a plastic bag, sterilizing and cooling, and inoculating with Phellinus baumii spores; (2) fungus management: avoiding light cultivation of the inoculated plastic bag; when the fungus management is performed for 30-35 days, the fungus bag is longitudinally squeezed and shortened, and the cultivation is continued to enter the fruiting management; (3) fruiting management: transferring the matured Phellinus baumii fungus bag into a fruiting greenhouse, drawing a rectangular fruiting opening in the middle of the fungus bag, and performing the Phellinus baumii fruiting management; (4) harvesting: obtaining the Phellinus baumii fruiting body after 100-120 days after the opening is drawn. The present application provides a cultivation method of high-yield and high-quality Phellinus baumii, which significantly improves the biological conversion rate and effective component content of Phellinus baumii by optimizing the culture medium components and ratio, and solves the technical problems of low yield and low effective component content in traditional cultivation.

This invention relates to the field of microbial fermentation technology, and particularly to a co-culture fermentation broth of medicinal and edible fungi with whitening effects, its preparation method, and its application. The preparation method of the co-culture fermentation broth of medicinal and edible fungi with whitening effects includes: inoculating the seed liquid of a first fungus and the seed liquid of a second fungus into a co-fermentation medium for fermentation and co-culture to obtain the co-culture fermentation broth of medicinal and edible fungi with whitening effects; the first fungus includes at least one of *Ganoderma lucidum* strain, *Trametes versicolor* strain, *Poria cocos* strain, *Auricularia auricula-judae* strain, *Phellinus linteus* strain, and *Aspergillus schavar*; the second fungus includes *Schizophyllum commune*. This invention co-cultures *Schizophyllum commune* with other specific strains and found that co-culturing the two strains can exert a synergistic effect, thereby significantly improving the tyrosinase inhibition effect of the fermentation broth. The optimal combination of fermentation broth can achieve a tyrosinase inhibition rate of over 90%, and even 1 wt% of the fermentation broth has a tyrosinase inhibition effect of approximately 90%.