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Separating technology of aristolane type sesquiterpene in phellinus igniarius

A Phellinus and sesquiterpene technology, applied in the separation/purification of carbonyl compounds, horticulture, botany equipment and methods, etc.

Inactive Publication Date: 2013-05-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Separating technology of aristolane type sesquiterpene in phellinus igniarius
  • Separating technology of aristolane type sesquiterpene in phellinus igniarius

Examples

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example 1

[0028] Phellinus crude extract, prepared by the following method:

[0029] (1) The fermentation medium formula is in grams / 100 milliliters:

[0030] Corn Starch 1% Glucose 1%

[0031] Peptone 0.1% Yeast Extract 0.1%

[0032] Magnesium sulfate 0.1% Potassium dihydrogen phosphate 0.01%

[0033] (2) Phellinus bacterium classification is inoculated in the Erlenmeyer flask that liquid medium is housed with 25 ℃ of temperature with 25 ℃ of temperature, and the rotating speed of shaking flask is 110r / min, under the condition of pH 7, vibrating culture 7 days; When the value drops to 3, the seeds in the shake flask are inoculated into the culture solution of a 50L fermenter, with a temperature of 25°C, a fermenter pressure of 0.1 kg / cm2, a pH of 3, an air flow of 0.5-1.1vvm, and a stirring speed of 100 rpm Under the condition of 1 / min, after culturing for 7 days, the Phellinus mycelium can be used to ferment the whole liquid to prepare the Phellinus crude extract;

[0034] (3) Tak...

example 2

[0040] Phellinus crude extract, prepared by the following method:

[0041] (1) The fermentation medium formula is in grams / 100 milliliters:

[0042] Corn Starch 3% Glucose 2%

[0043] Peptone 0.5% Yeast Extract 0.5%

[0044] Magnesium sulfate 0.5% Potassium dihydrogen phosphate 0.05%

[0045](2) Phellinus bacterium classification is inoculated in the Erlenmeyer flask that liquid culture medium is housed with 30 ℃ of temperature with 30 ℃ of temperature, and the rotating speed of shaking flask is 180r / min, under the condition of pH6, vibration culture 15 days; When the temperature drops to 2.5, inoculate the seeds in the shake flask into the culture solution of a 50L fermenter, with a temperature of 30°C, a fermenter pressure of 0.2 kg / cm2, a pH of 3, an air flow of 0.5-1.1vvm, and a stirring speed of 180 rpm. The condition of dividing, cultivating 15 days, can utilize Phellinus mycelia to ferment the whole liquid to prepare Phellinus crude extract;

[0046] (3) Take the Ph...

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Abstract

The invention discloses a separating technology of aristolane type sesquiterpene in phellinus igniarius (phellinus igniarius (Le*Fr)Quel, phellinus igniarius (BerketCurt) Teng, phellinus baumii, phellinus hartigii (AlleschetSchnabl)Imaz). The separating technology comprises the following steps of: firstly, preparing phellinus igniarius crude extract; secondly, carrying out positive-phase silica gel chromatography, using chloroform and carbinol to carry out gradient elution; thirdly, carrying out positive-phase silica gel chromatography again and using petroleum ether and acetone as eluants fourthly, carrying out chloroform-carbinol gel chromatography, carbinol gel chromatography and positive-phase silica gel chromatography in sequence; and finally, drying by distillation under reduced pressure to obtain the aristolane type sesquiterpene.

Description

technical field [0001] The invention belongs to the field of bioengineering pharmacy. Background technique [0002] Phellinus, fruiting body sessile, cap flat hemispherical or horseshoe-shaped, 2-12*3-21 cm, 1.5-10 cm thick, woody, light liver brown to dark gray or black, often cracked when old, without shell , there are fine hairs at the beginning, then become glabrous, with concentric ring edges. The edges are blunt, dark cinnamon to light brown, and the lower side has no hymenium. The flesh is dark brown, hard, woody. The tube and the flesh are nearly the same color, Multi-layered, but the layers are not obvious, and the old tube layer is filled with white hyphae. The tube mouth is rusty brown to caramel, round, 4-5 per mm. The spores are nearly spherical, smooth, colorless, 5-6*3 -4 microns. Setae are sharp at the top and swollen at the base, 10-25*5-7 microns. Mycelia are unbranched, without septa, 3-5 microns in diameter. [0003] At present, there are many purificat...

Claims

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Application Information

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IPC IPC(8): C07C49/643C07C45/79A01G1/04
Inventor 宋爱荣赵晨孙效乐王光远秦丹
Owner QINGDAO AGRI UNIV
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