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Bacteriostatic protective agent as well as preparation method and application

A protective agent and the resulting technology, applied in the biological field, can solve the problems of lack of broad-spectrum antibacterial properties and small range of action, and achieve the effects of broad market application value, protection of survival rate, and easy operation

Inactive Publication Date: 2016-04-20
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to solve the defects that the exogenous antibacterial components currently used in dairy products have no broad-spectrum antibacterial properties, a small range of action, and few types that can ensure safety and non-toxicity. The present invention provides A bacteriostatic protective agent and its preparation method and application are disclosed. The bacteriostatic protective agent is derived from microorganisms, has a wide range of action and broad-spectrum antibacterial properties, is natural, safe, and has a simple and reliable preparation method, and is suitable for large-scale production and in dairy products. product market application

Method used

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  • Bacteriostatic protective agent as well as preparation method and application
  • Bacteriostatic protective agent as well as preparation method and application
  • Bacteriostatic protective agent as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The inhibition of embodiment 1 bacteriostatic protective agent to Gram-negative bacteria

[0030] After the Phellinus fungus is cultivated to obtain the fruiting body, the fruiting body is crushed to about 1cm 3 granules, add 10 times the volume of deionized water to the dried crushed fruit body, extract with boiling water for 3 hours, and filter the residue for secondary extraction; combine the two extracts to concentrate under reduced pressure, and centrifuge at 4000r / min for 10min to remove the precipitate; Add ethanol to the concentrated solution until the final concentration of ethanol is 95%. After standing at room temperature for 24 hours, pour out the supernatant, centrifuge at 4000r / min for 10min to collect the precipitate, add deionized water to dissolve the precipitate, and centrifuge at 4000r / min for 10min Remove the insoluble matter, take the supernatant; freeze-dry the supernatant, take the freeze-dried extract as the antibacterial protective agent, and pr...

Embodiment 2

[0036] The inhibition of embodiment 2 bacteriostatic protective agent to Gram-positive bacteria

[0037] After the Phellinus fungus is cultivated to obtain the fruiting body, the fruiting body is crushed to about 1cm 3 Add 25 times the volume of deionized water to the dried crushed fruiting bodies, extract with boiling water for 1 hour, and filter the residue for secondary extraction; combine the two extracts and concentrate under reduced pressure, centrifuge at 4000r / min for 10min to remove the precipitate Add ethanol to the concentrated solution until the final concentration of ethanol is 65%. After standing at room temperature for 24 hours, pour out the supernatant, centrifuge at 4000r / min for 10min to collect the precipitate, add deionized water to dissolve the precipitate, and centrifuge at 4000r / min Remove the insoluble matter for 10 minutes, and take the supernatant; dialyze the supernatant for 24 hours, freeze-dry the solution after dialysis, take the freeze-dried extr...

Embodiment 3

[0042] The inhibition of embodiment 3 bacteriostatic protective agent to Gram-positive bacteria

[0043] After the Phellinus fungus is cultivated to obtain the fruiting body, the fruiting body is crushed to about 1cm 3 Add 15 times the volume of deionized water to the dried crushed fruit body, extract with boiling water for 2 hours, and filter the residue for secondary extraction; combine the two extracts and concentrate under reduced pressure, centrifuge at 4000r / min for 10min to remove the precipitate Add ethanol to the concentrated solution until the final concentration of ethanol is 80%. After standing at room temperature for 24 hours, centrifuge at 4000r / min for 10min to collect the precipitate, add deionized water to dissolve the precipitate, and centrifuge at 4000r / min for 10min to remove the insoluble matter. clear; dialyze the supernatant for 12 hours, freeze-dry the solution after dialysis, take the freeze-dried extract as the antibacterial protective agent, and prep...

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Abstract

The invention discloses a bacteriostatic protective agent as well as a preparation method and an application thereof. The preparation method comprises steps as follows: Phellinus baumii fruiting bodies are added to water after being crushed, and boiling water extraction is performed; an obtained mixture is filtered, and residues and an extract liquid are obtained; after the extract liquid is concentrated, a supernatant is taken, and a concentrated liquid is obtained; ethyl alcohol is added to the obtained concentrated liquid, standing is performed, precipitates are collected, water is added for dissolution, a supernatant is taken, and the bacteriostatic protective agent is obtained. The preparation method is convenient to operate and can better meet the requirement of industrial production. The bacteriostatic protective agent prepared with the preparation method is derived from edible fungi, is safe and reliable, has broad spectrum bacteriostatic performance, has no inhibition effect on growth of probiotics, increases the survival rate of beneficial microorganisms while inhibiting harmful microorganisms and has broad market prospect and application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacteriostatic protective agent, a preparation method and an application. Background technique [0002] Currently, 2% of the world's population suffers from foodborne diseases every year, and 25% of the world's food supply is contaminated by microorganisms. Although our country is becoming more and more open to food laws and regulations, the requirements for food safety are becoming more and more stringent. With the improvement of people's consumption level and awareness, natural and safe food has become the main consumption trend of consumers. The natural antibacterial ingredients are mainly divided into antibacterial ingredients of animal origin, antibacterial ingredients of plant origin and antibacterial ingredients of microbial origin. Due to the advantages of easy access, wide sources, low cost, and easy expansion of cultivation, microbial-derived bacteriostatic ...

Claims

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Application Information

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IPC IPC(8): A23C3/08
CPCA23C3/08A23C2240/15
Inventor 王钦博杨焱杭锋刘振民齐晓彦穆海菠王国骄洪青雍靖怡
Owner BRIGHT DAIRY & FOOD
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