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Preparation method of Phellinus linteus mycelium

A technology of Phellinus linteus mycelium and Phellinus linteus, applied in the biological field, can solve the problems of in-depth research and low total RNA concentration, and achieve the effect of easy-to-understand principle and simple operation.

Inactive Publication Date: 2012-11-28
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The mycelium collected after cultured by this method, because of the solid medium attached, the concentration of total RNA extracted is not high, which directly affects the subsequent in-depth research

Method used

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  • Preparation method of Phellinus linteus mycelium

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Embodiment 1

[0024] Embodiment 1: the preparation method of a kind of Phellinus mycelia of the present invention, the steps are as follows:

[0025] Step 1: Phellinus activation:

[0026] Use the slant of the potato dextrose agar medium test tube to activate the Phellinus strains stored in the refrigerator. First, place the Phellinus strains taken out of the 4°C refrigerator at room temperature for a period of time until it is consistent with the room temperature. Carry out potato dextrose agar medium test tube slant inoculation in the ultra-clean workbench. Each test tube slant is only connected to one point, covered with a rubber stopper, and placed in a 25°C incubator for 5-7 days at a constant temperature and protected from light;

[0027] Step 2: Plate culture on potato dextrose agar medium:

[0028] The activated Phellinus bacterium is transferred to the potato dextrose agar medium plate, and in the ultra-clean workbench, pick the Phellinus mycelium that grows vigorously on the edge...

Embodiment 2

[0038] Embodiment 2: the preparation method of a kind of Phellinus mycelia of the present invention, the steps are as follows:

[0039] Step 1: Prepare Phellinus mycelium slant medium:

[0040] Configure Phellinus mycelia medium PDA medium IL described in the present invention, each component weight in every liter is: potato 200g (peeled), glucose 20g, agar 15g. Dispense the culture medium into test tubes while hot, about 5ml per tube, cover with rubber stoppers, and sterilize with high pressure steam at 121°C for 15 minutes. After taking it out, put it on a slope while it is hot, cool it, and set it aside.

[0041] Step 2: Activation of strains:

[0042] Activate the Phellinus strains stored in the refrigerator with the prepared PDA medium slope, first place the Phellinus strains taken out of the refrigerator at 4°C for a period of time at room temperature until it is consistent with the room temperature, and then in the Inoculate the PDA slant in the ultra-clean workbench,...

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Abstract

The invention aims at providing a preparation method of a Phellinus linteus mycelium. The method comprises the following steps of: activating Phellinus linteus spawn; executing plate cultivation of potato dextrose agar culture medium; scraping the Phellinus linteus mycelium to inoculate the scraped Phellinus linteus mycelium to the potato dextrose agar culture medium for shake cultivation; collecting the Phellinus linteus mycelium; extracting Phellinus linteus mycelium total RNA; and observing result via agarose gel electrophoresis. The spawn used in the preparation method is the Phellinus linteus spawn (Phellinus baumii Pilate) collected in the CCTCC (China Center for Type Culture Collection), and the collection number is Phellinus linteus DL101 CCTCC M 2011137. The preparation method issimple to operate and has straightforward principle, and a simple and convenient method for obtaining large-scale high-purity Phellinus linteus mycelium and high-quality Phellinus linteus total RNA in the future is provided, and the basis for fundamental research and application development of the Phellinus linteus is also provided.

Description

(1) Technical field [0001] The invention relates to biotechnology, in particular to a method for preparing Phellinus mycelium. (2) Background technology [0002] Phellinus belongs to Basidiomycotas, Hymenomycetes, Aphyllophorales, Hymenochae taceae, and Phellinus Quel. The scientific name is Phellinus baumii, and the Chinese name is Phellinus baumii. It is commonly known as Phellinus baumii because of its bright yellow fruiting body, also known as Sang Er. It is a perennial rare medicinal fungus. Phellinus is one of the best anti-tumor fungi internationally recognized, without any toxic effects, is a precious medicinal fungus with great development value, and has become a research hotspot of medicinal fungi. [0003] The high-quality extraction of Phellinus chinensis total RNA is the basis for in-depth study of the molecular mechanism of Phellinus chinensis anti-cancer effect, but domestic and foreign scholars have little research on this content. In the existing productio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 邹莉王志英孙婷婷刘志华
Owner NORTHEAST FORESTRY UNIVERSITY
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