Method for producing sterilized miniascape
A production method and bonsai technology, applied in botany equipment and methods, soilless cultivation, decorative art, etc., can solve the problems of smelly, rotten, etc., and achieve the effect of high technological content, promotion of splitting, and good market prospects
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example 1
[0042] Example 1: a kind of foliage plant aseptic bonsai (described foliage plant such as red pitcher plant, cocoon, oncidium, dendrobium orchid, variegated taro...), its preparation method is as follows:
[0043] 1. Disinfection of explants: After washing the explants of plants (including seeds, leaf buds, leaves, etc.) on the surface of washing powder, soak them in 75% alcohol for 30 seconds, soak them in 0.1% mercury liter for 5-20 minutes, and then soak them in sterile water. Inoculate after washing 3-5 times;
[0044] 2. Preparation of culture medium: according to the formula, first weigh agar powder and white sugar, add them to 300 ml of distilled water and boil them, then add the other ingredients in the formula in full, and add 3 mg / L of red food coloring; the above ingredients After dissolving and mixing, add distilled water to 1000 ml, and adjust the pH to 5.8 with 3% NaOH or 1N HCl, then divide the medium into transparent containers and seal them. At 1.1kg / cm 2 St...
example 2
[0062] Example 2: a kind of preparation method of ornamental plant aseptic bonsai, comprises the following steps: (described ornamental plant, as cockscomb, carnation, morning glory...):
[0063] Step 1 is the same as step 3 and the first embodiment, and the method for preparing medium in step 2 is as follows:
[0064] According to the formula of the culture medium, first weigh agar powder and white sugar, add them to 300 ml of distilled water and boil, then add the other ingredients in the formula; add lemon yellow food coloring 60mg / L; dissolve and mix the above ingredients Add distilled water to 1000 ml, and adjust the pH to 5.8 with 3% NaOH or 1N HCl, then divide the medium into transparent containers and seal them. At 1.1kg / cm 2 Sterilize under high pressure for 20 minutes, cool and solidify for later use. During autoclaving, high temperature and high pressure resistant decorative materials can be autoclaved at the same time. Described culture medium formula is as foll...
example 3
[0080] Example 3: a kind of mixed aseptic bonsai (the mixed aseptic bonsai, such as figure 2 Shown, with the bonsai mixed inoculation of the aseptic seedling of cocoon taro, acacia and cockscomb), the preparation method comprises the following steps:
[0081] Step 1 is the same as step 3 and the first embodiment, and the method for preparing medium in step 2 is as follows:
[0082] According to the formula of the culture medium, first weigh agar powder and white sugar, add them to 300 ml of distilled water and boil them, then add the other ingredients in the formula; add 3 mg / L of blue food coloring, (or do not add coloring ), after dissolving and mixing the above ingredients, add distilled water to 1000 ml, then adjust the pH to 5.8 with 3% NaOH or 1N HCl, then divide the medium into transparent containers and seal them. At 1.1kg / cm 2 Sterilize under high pressure for 20 minutes, cool and solidify for later use. During autoclaving, high temperature and high pressure resis...
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