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Method for breeding new flower-shaped chrysanthemum

A chrysanthemum and flower-shaped technology, applied in horticultural methods, botanical equipment and methods, angiosperms/flowering plants, etc., can solve problems such as research reports that have not been applied

Inactive Publication Date: 2008-04-09
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the study of chrysanthemum flower type and plant shape, although it has been reported that the genes CY and DICH that control the symmetry of petal morphology have been cloned from Antirrhinum grass (Rhoda. Molecular inheritance of flower development (Xu Zhihong, Liu Chunming editor-in-chief). Plant development Molecular mechanism, Beijing: Science Press, 1997, 89-106), but there is no research report on its further application

Method used

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  • Method for breeding new flower-shaped chrysanthemum
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  • Method for breeding new flower-shaped chrysanthemum

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Experimental program
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Embodiment 1

[0032] Embodiment 1, the cultivation method of variant 04M, carry out the following steps successively:

[0033] 1), cutting young flower buds with a diameter of 0.45 to 0.55 cm from the natural growth of the small purple chrysanthemum plant as explants;

[0034]2), the above-mentioned flower buds are first rinsed twice with soapy water, and then soaked in 70% alcohol for 30 seconds; then placed in a sodium hypochlorite solution with a concentration of 0.7% for sterilization for 20 minutes, and finally rinsed twice with sterile distilled water;

[0035] 3), dry the flower buds after the above-mentioned disinfection treatment, and carry out the following induction differentiation treatment in the culture bottle:

[0036] The flower buds were cut evenly into 4 pieces and inoculated on the induction medium after the outer calyx was removed. The induction medium was: MS+6-BA 3mg / L+NAA 0.5mg / L, and the culture conditions were: the light intensity was 2000Lux, The light time is 12h...

Embodiment 2

[0040] Embodiment 2, a kind of breeding method of new flower type chrysanthemum: take the variant 04M that embodiment 1 gains as male parent, take common chrysanthemum variety Hangbaiju as female parent, artificially remove the pollen of Hangbaiju, and the mutant 04M The pollen is pollinated to the stigma of the pistil of Chrysanthemum chrysanthemum, and seeds are obtained. Plants grow after the seeds are sown, and individual plants with variated flower types are obtained, thereby obtaining new chrysanthemum breeding materials.

Embodiment 3

[0041] Embodiment 3, a kind of breeding method of new flower type chrysanthemum: utilize mutant 04M as female parent, take common chrysanthemum variety Hangbaiju as male parent, artificially remove the pollen of mutant 04M, pollinate the pollen of Hangbaiju to the mutant The stigma of the pistil of 04M obtains seeds, and plants grow after the seeds are sown, and a single plant with a mutated flower type is obtained, and new chrysanthemum breeding materials are obtained.

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Abstract

The invention discloses a novel chrysanthemum breeding method, which includes the following steps: 1) the young flower buds whose diameters are 0.45-0.55cm from the naturally grown small violet chrysanthemum plants are scissored as the explants; 2) the flower buds are rinsed by soapy-water, soaked by alcohol, sterilized by sodium hypochlorite in turn, and then rinsed by asepsis distilled water; 3) the sterilized flower buds are sucked up water,, induced and separated in a cultivating bottle; 4) the separated tube seedlings are cultivated into young plants; 5) the young plants are transplanted into a bowl filled with cultivation mud for breeding; 6) the grown plants whose leaf is ovate-lanceolate and stem is rhombuses, and inflorescence is verticillaster are selected among the survived transplanted plants, namely the variant 04M; 7) after sexual hybridization or asexuality graft of the variant 04M, then the novel chrysanthemum is obtained. The chrysanthemum cultivated with the method of the invention is verticillaster.

Description

technical field [0001] The invention relates to a method for breeding new flower-type varieties of chrysanthemums. Background technique [0002] Chrysanthemum (Dendranthema morifolium) belongs to the plant of Compositae Chrysanthemum. It is native to my country. It is one of the top ten traditional famous flowers in my country and one of the four famous flowers in the world. It is also a famous flower integrating cultural significance and economic value. It has edible and medicinal properties. Use value. Chrysanthemum has rich variety resources, profound cultural connotation and broad mass base in my country. Chrysanthemum occupies a very important position in the flower production of our country, and its industrial benefits occupy a large proportion in the flower trade volume of my country (Li Xinlei, Chen Fadi . Chrysanthemum germplasm resources and genetic improvement research progress. Botany Bulletin, 2004, 21(4): 392-401). [0003] Breeding novel flower varieties has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00C12N5/04A01G31/00A01H5/00
Inventor 向太和王利琳庞基良
Owner HANGZHOU NORMAL UNIVERSITY
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