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Microbial preparation for improving eel liver growth and its making method

A microbial preparation, growth and development technology, applied to medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve problems such as increased antibiotic residues in eel, decreased growth water quality, and excessive nitrite content, etc., to achieve guaranteed Physical health, synthesis boosting, targeted effects

Inactive Publication Date: 2007-11-21
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because eel has the above special effects, its breeding prospects are broad. However, due to the use of a large amount of protein feed and the addition of antibiotics in recent years, the residues of antibiotics in eels have increased, and the quality of the growing water has seriously declined. The content of ammonia nitrogen and nitrite has seriously exceeded the standard. Fish body mainly synthesizes special protein and vitamin A - the liver grows slowly, which seriously reduces the quality of food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1: Breeding of production strains

[0035]a. Strain screening: Homogenous strains were obtained from eel intestines, and lactic acid bacteria were isolated using MRS lactic acid bacteria medium, and Lactobacillus plantarum (ATCC8014) Lactobacillus plantarum, Lactobacillus casei (ATCC7469) Lactobacillus casei and licheniformis (13123) Bacillus licheniformis were screened out ; Isolate Saccharomycescerevisiae (IFO0203) brewer's yeast with yeast selection medium in beer fermentation broth;

[0036] b. The strains were inoculated in broth medium, cultivated at 35°C for 24 hours, and inoculated respectively on Meclary agar, nutrient agar (LB medium) and Lactic-bacteria Medium I (lactic acid bacteria medium), Carry out streak culture, select brewer's yeast, Lactobacillus plantarum, Lactobacillus casei and Bacillus licheniformis with fast growth and obvious cell characteristics, and store them in the laboratory;

[0037] c. Seed production: transfer the strains into eggp...

Embodiment 2

[0056] The first step: selection and breeding of production strains: same as Example 1

[0057] The second step: separate and compound culture of beneficial strains:

[0058] a. Compound culture of lactic acid bacteria and yeast: inoculate mixed lactic acid bacteria and yeast at 121°C, sterilize at 30 minutes high temperature, make molasses 18%, urea 4%, yeast powder 3%, potassium dihydrogen phosphate 1%, disodium hydrogen phosphate 1%, sterile water 73% in the culture medium, stir in the fermenter for 30min, its rotating speed is 220r / min, after stirring evenly, put it into a sterilized plastic bucket and let it stand for 15 days, and measure Ph every day during the standstill value and release the produced gas to prevent the feed tank from bursting, and the fermentation ends when the Ph reaches 3.5;

[0059] b. Cultivation of Bacillus licheniformis alone: ​​Inoculate the lawn of eggplant bottles in a culture medium sterilized at 121°C for 30 minutes, the stirring speed is 2...

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Abstract

The invention is concerned with a microorganism feedstuff additive to promote the growth of eels, especially to improve the compound function to liver. Culture one or some of the selected lichen bacillus, plant lactobacillus, lactobacillus casei and beer yeast. Carry domesticationhe and train the multiple bacteria and zymosis with culture medium and asepsis water, dry quickly with low temperature through drying mechine, add special carrier, crush and sift out to get production. It has the greatest amout of live bacterium and active metabolized produce, and the production has microorganism with high concentration and abundant active metabolized stuff. Those bacteria and metabolized stuff can improve the intestines function of eels to speed the composing of bile and special protein divided form liver. It also improves the water for living and it is a kind of zoology agriculture production.

Description

technical field [0001] The invention relates to a microbial preparation for promoting the growth and development of the eel liver and a preparation method thereof, which can be added to common eel feed, and special microorganisms and metabolites are used to promote the development and growth of the eel liver, thereby improving the special protein and vitamin A production ability of the eel , belongs to the technical field of agricultural biological products. Background technique [0002] Eel is a kind of precious edible fish, which has the functions of tonifying deficiency and nourishing blood, dispelling dampness and anti-tuberculosis. It is a good nutrient for patients with chronic illness, weakness, anemia, and tuberculosis. Eel contains a very rare Xihe Locke protein, which has good effect of strengthening essence and kidney. Eel is an aquatic product rich in calcium. Regular consumption can increase blood calcium and strengthen the body. Eel liver is rich in vitamin A ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/064A61P1/16C12N1/16C12N1/20A61K35/74A61K35/742A61K35/747
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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