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Cell analysis technology based on film fiber material micro fluidic chip

A microfluidic chip and film fiber technology, applied in the field of cell analysis technology, to achieve the effects of low consumption of cells and reagents, easy aseptic treatment, and spiritual pattern patterns

Inactive Publication Date: 2013-08-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of this new type of microfluidic "paper" chip for cell analysis is still in the blank stage.

Method used

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  • Cell analysis technology based on film fiber material micro fluidic chip
  • Cell analysis technology based on film fiber material micro fluidic chip
  • Cell analysis technology based on film fiber material micro fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Using the microfluidic chip designed and manufactured by the laboratory, the configuration is as follows: figure 1 shown. After surface modification with type 1 collagen, ACCM cells were inoculated on the chip at a seeding density of 1×10 5 cells / mL, after 24 hours of cell attachment, replace with 1640 medium containing 0 micromol per liter, 1 micromol per liter, and 5 micromol per liter of actinomycin D. After 24 hours, cell death, apoptosis, etc. Related staining, photographed under a microscope, and analyzed by Image Pro software. The result is as image 3 shown.

Embodiment 2

[0020] Using the microfluidic chip system designed and produced by the laboratory, the configuration is as follows: figure 2 shown. Laminin-modified chips were used to inoculate GES at a density of 1×10 5 cells / mL, after 24 hours of cell attachment, the high-sugar DMEM medium containing 0 micromole per liter, 1 micromole per liter, and 5 micromole per liter of actinomycin D was used for cell death and apoptosis after 24 hours. Dying and other aspects were stained, photographed under a microscope, and analyzed by Image Pro software.

Embodiment 3

[0022] Using the microfluidic chip system designed and produced by the laboratory, the configuration is as follows: figure 1 shown. The chip was inoculated with mouse bone marrow mesenchymal stem cells at a seeding density of 1×10 5 cells / mL, after 24 hours of cell attachment, replace with a variety of compatible LG-DMEM+10%FBS+1μM dexamethasone+insulin adipogenic differentiation medium, change the medium every day, and stain with adipose Oil Red after 7 days, Photographs were taken under a microscope and analyzed by Image Pro software. The result is as Figure 5 As shown, mesenchymal stem cells undergo different adipogenic and osteogenic differentiations.

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Abstract

The present invention provides a cell analysis technology based on a film fiber material micro fluidic chip. The technology mainly comprises three basic aspects: 1, a film fiber material micro fluidic chip production technology, 2, an asepsis technology and surface modification of the film fiber material micro fluidic chip, and 3, cell culture, stimulation, and analysis and evaluation of the film fiber material micro fluidic chip. The technology has advantages of simple operation, low cost, low reagent and cell consumption, and the like, and is a new technology widely used for various cell analyses.

Description

technical field [0001] The invention mainly relates to the technical field of microfluidic chips of film fiber materials, and in particular provides a cell analysis technology based on microfluidic chips of film fiber materials. Background technique [0002] As an important science and technology in this century, microfluidic chip laboratory has demonstrated its unique advantages in many fields including chemistry, biology, medicine, etc. With the characteristics of fast heat and mass transfer, high flux and integration, it has become an important platform for the research of the new generation of cells. With the development of the past two decades, cell research based on the microfluidic chip system has made breakthroughs. Many cell-related A variety of applied research has begun to appear in the field of chips, among which cell analysis technology has become an important branch of research that has attracted much attention. At present, most of the cell analysis platforms ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12Q1/68G01N33/68
Inventor 秦建华高兴华张敏张旭马慧朋
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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